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Method for continuously performing enzymolysis resolution on mixed-rotation pantolactone by utilizing fixed bed device

A technology of pantolactone and fixed bed, which is applied in the field of enzyme engineering, can solve the problems of shortening the service life of enzymes, damaging the integrity of enzymes, and shearing damage of enzymes, achieving less destructiveness, prolonging service life, and ensuring integrity Effect

Inactive Publication Date: 2020-11-24
CHONGQING HONOROAD ANIMAL HEALTH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the separation of gyropantoate lactone is produced intermittently. The mechanical agitation during the separation in this way causes shear damage to the enzyme, damages the integrity of the enzyme, and shortens the service life of the enzyme.

Method used

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  • Method for continuously performing enzymolysis resolution on mixed-rotation pantolactone by utilizing fixed bed device

Examples

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Embodiment 1

[0024] A method for continuously enzymatically decomposing vortex pantolactone using a fixed bed device described in this embodiment comprises the following steps:

[0025] (1) Stir and mix 7.5 t of immobilized enzyme and 5 t of diatomaceous earth (the ratio is 3:2) and add it into a fixed bed with an inner diameter of 2 meters, a height of 5 meters, and a diameter-to-height ratio of 2:5 , after covering the sieve plate, add 3 t of river sand for packing;

[0026] (2) Continuously transport the aqueous solution of hydropantolactone at a rate of 300 L / min through the fixed bed device for enzymatic decomposition, and adjust the pH of the aqueous solution of hydropantolactone with ammonia water so that when it passes through the fixed bed The pH is stable between 6.3 and 7.5. After the separation is completed, the enzymatic hydrolysis solution flows out from the bottom of the fixed bed, and samples are taken at the sampling port at the bottom to detect the optical rotation of th...

Embodiment 2

[0030] A method for continuously enzymatically decomposing vortex pantolactone using a fixed bed device described in this embodiment comprises the following steps:

[0031] (1) Stir and mix 4 t of immobilized enzyme with 7.5 t of diatomaceous earth (the ratio meets 8:15) and add it into a fixed bed with an inner diameter of 2 meters, a height of 4 meters, and a diameter-to-height ratio of 1:2 , after covering the sieve plate, add 1 t of river sand for packing;

[0032] (2) Continuously transport the aqueous solution of hydropantolactone at a rate of 200 L / min through the fixed bed device for enzymolysis and splitting, adjust the pH of the aqueous solution of hydropantolactone with ammonia water, and make it pass through the fixed bed The pH is stable between 6.5 and 7.2. After the separation is completed, the enzymatic hydrolysis solution flows out from the bottom of the fixed bed, and samples are taken at the sampling port at the bottom to detect the optical rotation of the ...

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Abstract

The invention relates to the field of enzyme engineering, provides a method for continuously performing enzymolysis resolution on mixed-rotation pantolactone by utilizing a fixed bed device, and particularly relates to development of a hydrolase fixed bed. The method comprises the following steps: uniformly mixing immobilized enzyme and diatomite, filling a column with the mixture, and packing andpressing river sand to prepare the fixed bed. According to the method, the destructive effect of mechanical stirring on the enzyme is greatly alleviated, the service life of the enzyme is prolonged,the resolution process is continuous, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to a method for continuously enzymatically decomposing vortex pantolactone by using a fixed bed device. Background technique [0002] D-pantothenic acid (chemical name: (+)2,4-dihydroxy-3,3-dimethylbutyric acid) is a key intermediate in the production of calcium D-pantothenate series products, through the resolution of vortex pantothenic acid Lactones are obtained. Most of the resolution methods of diced pantolactone are chemical methods, which have the disadvantages of expensive resolving agent, high production cost, small production volume, and poor optical purity of the product. Among the bio-resolution methods, the use of microbial enzymatic methods to resolve gyro-pantoate lactones has become an important research direction because of its environmental pollution and less toxicity. [0003] One way of microbial enzymatic resolution of swirling pantoolact...

Claims

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Application Information

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IPC IPC(8): C12P41/00C12P7/62C12M1/40
CPCC12M21/18C12P41/005C12P7/62
Inventor 赵德蒋佳璇任敏
Owner CHONGQING HONOROAD ANIMAL HEALTH
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