High-retainment dianthus caryophyllus seedling breeding method

Abstract

The invention relates to the technical field of plant rapid propagation, and particularly discloses a high-retainment dianthus caryophyllus seedling propagation method which comprises the following steps: inoculating a dianthus caryophyllus branch serving as an initial explant onto a fixed bud induction culture medium, and performing adventitious bud induction pre-culture and adventitious bud induction culture to obtain clustered buds. Test results show that the adventitious buds can be directly induced to form buds only by about 2 weeks, the growth speed is remarkably higher than that of callus induced bud formation, and the direct induced bud formation has good hereditary stability and has good application and popularization prospects.

Description

technical field [0001] The invention relates to the technical field of rapid plant breeding, in particular to a method for breeding high-fidelity carnation seedlings. Background technique [0002] Carnation (Dianthus caryophyllus L.), also known as carnation, is of high ornamental value. It is one of the four major fresh cut flowers in the world. It is the main flower of Mother’s Day in more than 100 countries including China, Japan, and the United States, and the national flower of 6 countries including Spain and Turkey. , deeply loved by people all over the world. In recent years, the planting area of ​​Carnation in my country has continued to expand, and domestic sales and foreign exports have continued to rise, but there are not many high-quality new varieties in China. Carnation is mainly propagated by cuttings and divisions, but the reproduction coefficient is low, and the germplasm resources are degraded due to its susceptibility to virus infection. Plant in vitro r...

AI Technical Summary

Problems solved by technology

Under normal circumstances, the large-scale breeding of high-quality seedlings of Carnation at home and abroad mainly relies on in vitro rapid propagation technology (Qian Lijuan, Zhang Shanshan, Jia Aiping. Effects of different hormone ratios on carnation shoot tip tissue culture[J ].Agricultural Science Research, 2013(3):35-37; Zhou Ruijin, Yang Jing, Kong Xiaoping. Effects of Plant Growth Regulators on Carnation in Vitro Culture[J].Modern Horticulture, 2013(24):5-6 ), but most of them are induced by callus to produce adventitious buds, which often cause a certain amount of mutant strains and affect the uniformity of seedlings (Zhang Yuyuan, Du Qingye. The influence of different culture conditions on the vitrification of Carnation seedlings[ J]. Friends of farmers getting rich, 2015(22):90-91+169.)

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