Method for simultaneously determining nucleoside and flavonoid components in trichosanthes kirilowii maxim medicinal material and application of method

Abstract

The invention provides a method for simultaneously determining nucleoside and flavonoid components in a trichosanthes kirilowii maxim medicinal material and application of the method, and belongs to the technical field of detection and analysis. The invention successfully establishes a method for qualitatively and quantitatively determining nucleoside and flavonoid components in trichosanthes kirilowii maxim by combining ultrasonic-assisted extraction with a ZIC-HILIC-DAD technology. The method is rapid, simple and convenient, and can realize simultaneous determination of nucleoside and flavonoid components in trichosanthes kirilowii maxim. Content determination results show that the contents of the two components in the trichosanthes kirilowii maxim in the Shandong production area and theHebei production area have a certain difference, and the content of nucleoside components in the trichosanthes kirilowii maxim in the Shandong production area is obviously higher than that in the Hebei production area. Overall distinguishing of the samples of the two production areas is achieved by combining principal component analysis and system clustering analysis. Research results provide analysis method support for guaranteeing the quality control level of planting and processing of trichosanthes kirilowii maxim medicinal materials, and therefore the technical scheme has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a method for simultaneously measuring nucleosides and flavonoids in Trichosanthes medicinal material and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Trichosanthes is the dry mature fruit of Trichosanthes rosthornii Harms of Cucurbitaceae plant Trichmanthes kiriloxvii Maxim. It is sweet in nature, slightly bitter and cold in taste, and has the functions of clearing away heat and eliminating phlegm, widening the chest and dispelling stagnation, moistening dryness and smoothing the intestines. It is often used in Chinese medi...

AI Technical Summary

Problems solved by technology

The inventors found that at present, the quality control of Trichosanthes triolium in "Chinese Pharmacopoeia" only measures the content of 3,29-dibenzoylcitrenetriol in the seeds of Trichosanthes triolium, which cannot fully reflect the overall quality of Trichosanthes medicinals

The existing studies on the determination of the content of Trichosanthes medicinal materials mostly focus on a certain type of ingredients, and there are few reports on the detection of nucleosides and flavonoids, two important biologically active ingredients in Trichosanthes

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