Ex-TTF-aptamer conjugate, preparation method thereof and electrochemical sensor prepared from ex-TTF-aptamer conjugate

Abstract

The invention provides a preparation method of an ex-TTF-aptamer conjugate. The method comprises the following steps: adding a TTF compound and an azide into a tert-butyl alcohol solution, then addingN,N-diisopropylethylamine and copper iodide, performing full stirring and extracting to obtain an organic phase, washing the organic phase, and then performing drying, filtering and concentrating toobtain an ex-TTF derivative; and adding a dimethyl sulfoxide solution into the ex-TTF derivative for dissolving to obtain an ex-TTF derivative solution, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide into the ex-TTF derivative solution, performing ultrasonic dissolution, adding modified DNA molecules and a sodium bicarbonate solution, performing oscillation reaction, performing centrifuging after the reaction is finished, taking an upper-layer solution, and performing HPLC separation and purification on the upper-layer solution so as to obtain the ex-TTF-aptamer conjugate. The invention also provides an electrochemical sensor prepared from the ex-TTF aptamer conjugate. The electrochemical sensor can effectively realize the detection of small molecule drugs, and is not influenced by the pH change of the system.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an ex-TTF-aptamer conjugate, a preparation method thereof and an electrochemical sensor prepared using the same. Background technique [0002] With the development of technology and medical technology, the monitoring and management of one's own health has gradually attracted people's attention. Effective monitoring of human activity and physiological changes provides useful information and plays an important role in managing health and healthcare visits. Therefore, electrochemical sensors have successfully become a bridge between understanding human activities and physiological changes, and the detection of physiological environments has become particularly important. Physiological fluids contain a large number of health-related small molecules and trace analytes that can be used as biomarkers, and they have been proven to be very effective in predicting and diag...

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Problems solved by technology

The pH value between 6.0-7.5 is the optimal condition for enzyme-catalyzed electrochemical reactions, so the problem of pH value changes largely limits the development and application of enzyme biosensors

The performance of MB active molecules is relatively stable in a specific pH value detection system, but due to the participation of protons in the electrochemical reduction process of MB (refer to figure 2 ), the current signal output will be changed by the change of pH value, so it is impossible to achieve accurate detection in the pH value change system

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