A kind of ex-ttf-aptamer conjugate and its preparation method and electrochemical sensor prepared by using it

Abstract

The present invention provides a method for preparing ex-TTF-aptamer conjugates. The steps are as follows: adding TTF compound and azide compound into tert-butanol solution, then adding N,N-diisopropylethylamine and iodine copper is dissolved, fully stirred, and extracted to obtain an organic phase, and the organic phase is washed, then dried, filtered, and concentrated to obtain ex-TTF derivatives; dimethyl sulfoxide solution is added to the ex-TTF derivatives to dissolve, to obtain ex-TTF derivative solution, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to the ex-TTF derivative solution, sonicate Dissolve, then add the modified DNA molecule and sodium bicarbonate solution, shake the reaction, centrifuge after the reaction, take the upper layer solution, and perform HPLC separation and purification on the upper layer solution to obtain the ex-TTF-aptamer conjugate. The present invention also provides an electrochemical sensor prepared by using the ex-TTF-aptamer conjugate. The electrochemical sensor can effectively realize the detection of small molecule drugs, and is not affected by the pH change of the system.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an ex-TTF-aptamer conjugate, a preparation method thereof and an electrochemical sensor prepared using the same. Background technique [0002] With the development of technology and medical technology, the monitoring and management of one's own health has gradually attracted people's attention. Effective monitoring of human activity and physiological changes provides useful information and plays an important role in managing health and healthcare visits. Therefore, electrochemical sensors have successfully become a bridge between understanding human activities and physiological changes, and the detection of physiological environments has become particularly important. Physiological fluids contain a large number of health-related small molecules and trace analytes that can be used as biomarkers, and they have been proven to be very effective in predicting and diag...

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Problems solved by technology

The pH value between 6.0-7.5 is the optimal condition for enzyme-catalyzed electrochemical reactions, so the problem of pH value changes largely limits the development and application of enzyme biosensors

The performance of MB active molecules is relatively stable in a specific pH value detection system, but due to the participation of protons in the electrochemical reduction process of MB (refer to figure 2 ), the current signal output will be changed by the change of pH value, so it is impossible to achieve accurate detection in the pH value change system

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