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Antigen and egg yolk antibody for inhibiting multiple HPV viruses, and preparation methods and application thereof
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A technology of HPV virus and egg yolk antibody, which is applied in the field of biomedicine to prevent secondary HPV infection, improve the purity and inhibit the effect
Inactive Publication Date: 2020-12-01
YOULIKANG (JIANGSU) BIOPHARMACEUTICAL CO LTD
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Currently, there is a lack of specific drugs that target bacteria, fungi, and viruses at the same time
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[0046] Antigens for inhibiting various HPV viruses, said antigens comprising HPV L1, Clfa eggs of Staphylococcus aureus and Candida albicansLutein produced from the MP65 protein of bacteria;
[0047] The preparation method of the antigen is:
[0048] (11) Optimize the nucleotides corresponding to the respective capsid proteins of HPV16, HPV18, HPV6, HPV11, HPV31, HPV33, HPV52, HPV58, and HPV81, as well as the protein sequences of Staphylococcus aureus ClfA and Candida albicans MP65, and optimize to obtain a match the nucleotide sequence;
[0049] Among them, the protein sequences of HPV16, HPV18, HPV6, HPV11, HPV31, HPV33, HPV52, HPV58, HPV81, Staphylococcus aureus ClfA and Candida albicans MP65 are shown in sequence 1 to sequence 11 in the sequence table; The nucleotide sequence is sequentially shown in sequence 12 to sequence 22 in the sequence listing;
[0050] (12) Cloning the nucleotide sequence optimized in step (11) into pET2...
[0062] Adopt the preparation method of the yolk antibody that suppresses various HPV viruses by antigen immunization in embodiment 1, comprise the steps:
[0063] (21) The eleven kinds of antigens after genetic engineering were mixed in an appropriate proportion to prepare antigen LA for emulsification, wherein the emulsifier was Freund's complete adjuvant, Freund's incomplete adjuvant or aluminum hydroxideadjuvant;
[0064] (22) Select 10-40 week-old healthy egg-laying hens for isolation and rearing, and perform subcutaneous injections. Each hen is injected with 2-6 injection points, and the amount of antigen used for immunization is 100 μg / chicken. After the first immunization, it is strengthened every two weeks. immune once;
[0065] (23) Collect the eggs produced by the last immunization, and separate and purify egg yolk antibodies that inhibit multiple HPV viruses from the eggs produced.
[0066]The specific process ...
Embodiment 3
[0068] Embodiment 3: Preparation of egg yolk antibody freeze-dried powder
[0069] After separating the egg yolk, add 2 to 10 times the volume of deionized water, continue to stir for 30 molecules to 3 hours, place at 4°C for 10 to 30 minutes, and centrifuge at 8000 rpm for 15 minutes, take the supernatant egg yolk antibody solution, and freeze After drying, the lyophilized powder of egg yolk antibody can be obtained.
[0070] The yolk antibody containing various HPV viruses prepared by the method of the present invention can be applied to the preparation of gels, lotions, sanitary napkins, powders, suppositories, ointments, sprays, condoms, solid drinks, effervescent tablets, capsules, etc. product.
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Abstract
The invention discloses an antigen for inhibiting multiple HPV viruses. The antigen is xanthoglobulin prepared from respective capsidprotein HPV L1 of HPV16, HPV18, HPV6, HPV11, HPV31, HPV33, HPV52,HPV58 and HPV81, Clfa protein of staphylococcus aureus and MP65 protein of candida albicans; and the antigen is adopted for immunization to obtain the egg yolkantibody of the HPV virus. The egg yolkantibody prepared by the scheme provided by the invention can effectively block HPV infection and proliferation of staphylococcus aureus and candida albicans; and, compared with HPV inhibitors on themarket at present, the HPV inhibitor provided by the invention has a good inhibition effect on common HPV at present, has a broader spectrum of HPV inhibition ability, also has antibodies against staphylococcus aureus and candida albicans, and can better prevent secondary HPV infection of patients during bacterial and fungal infection.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an antigen and egg yolkantibody for inhibiting various HPV viruses, a preparation method and application thereof. Background technique [0002] Human papillomavirus (HPV) is a group of non-enveloped spherical, double-stranded circular DNA viruses belonging to the family Papillomaviridae, which infect human skin and mucosal epithelial tissues, and can induce verrucous hyperplasia and even benign or malignant tumors. According to the degree of malignancy of the lesions induced by HPV infection, it can be divided into high-risk HPV and low-risk HPV. High-risk HPV infection is closely related to the occurrence of cervical cancer, as well as the occurrence of mucosa-associated cancer and precancerous lesions. Among the malignant lesions associated with high-risk HPV infection, cervical cancer has the highest incidence rate, and its incidence rate in women is second ...
Claims
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Application Information
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