A method for preparing slices for observing turfgrass leaf vein network structure

A technology of network structure and turfgrass, which is applied in the field of film preparation for observation of turfgrass vein network structure, can solve problems such as the undisclosed determination method of turfgrass vein network structure, achieve adaptability, ensure decolorization effect, and universal adaptability Effect

Active Publication Date: 2022-04-15
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the research on the vein network structure mostly focuses on general broad-leaved plants and large monocotyledonous plants, as well as vein bookmarks and vein flowers, etc. There is no disclosed method for measuring the vein network structure of turfgrass

Method used

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  • A method for preparing slices for observing turfgrass leaf vein network structure
  • A method for preparing slices for observing turfgrass leaf vein network structure
  • A method for preparing slices for observing turfgrass leaf vein network structure

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preparation example Construction

[0044] A method for preparing slices for observing the vein network structure of turfgrass, specifically comprising the following method steps:

[0045] ⑴ Leaf collection and pretreatment

[0046]Select turfgrass leaves to be treated, soak them in distilled water for 10 minutes, take out the leaves, and let them stand on the filter paper for 10-20 seconds to remove the water and impurities on the surface of the leaves, and use transparent tape to remove the fluff on the front and back of the leaves. The length of treatment is 6 ~8cm, then apply colorless nail polish evenly on the front and back of the leaves, after it dries, remove the nail polish with scotch tape, and cut leaves 4-6cm long from the treated part;

[0047] ⑵Decolorization treatment

[0048] Soak the treated grass blades in 5% NaOH solution for 2 to 3 days, and change the soaking solution once in the middle;

[0049] ⑶ Vein dyeing and color separation

[0050] According to the type of lawn grass leaves, two m...

Embodiment 1

[0064] The present embodiment relates to a method for preparing slices for observing the vein network structure of bluegrass (Poapratensis), comprising the following steps:

[0065] 1) Leaf collection and pretreatment:

[0066] Select freshly mature bluegrass leaves and soak them in distilled water for 10 minutes; take out the leaves, use filter paper or paper-free toilet paper to deal with the water and impurities on the surface of the leaves, and then place them on the filter paper for 10-20 seconds; Stick the hair on the front and back of the middle part of the leaves, and the length of the treatment is 6-8cm; then apply the nail polish evenly on the front and back of the leaves, and after it dries, remove the nail polish with scotch tape. 4-6 cm long bluegrass leaves were cut from the treated parts as experimental materials.

[0067] 2) Decolorization of leaves: soak the treated bluegrass leaves in 5% NaOH solution for 2 days, and change the soaking solution once in the m...

Embodiment 2

[0072] This embodiment relates to a method for preparing slices for observing the vein network structure of creeping bentgrass (Agrostis stolonifera), the steps of which are the same as those in Embodiment 1. The only difference is that because the leaves of creeping bentgrass are smaller, the whole leaves are used as experimental materials in the leaf pretreatment stage, and the rehydration time is adjusted to 2h. The fabricated mounts were photographed under a LEICADM4000B LED optical microscope, and the leaf vein images were measured with LAS AF Lite image analysis software. Leaf vein images of creeping bentgrass such as figure 2 As shown, the transverse vein density is 0.904mm / mm 2 ;Large longitudinal vein density is 1.897mm / mm 2 ; The density of small longitudinal veins is 2.923mm / mm 2 . The ratios of transverse veins, large longitudinal veins and small longitudinal veins to the total leaf veins were 15.8%, 33.14% and 51.06%, respectively.

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Abstract

The invention relates to a method for preparing slices for observing the vein network structure of turfgrass, comprising: (1) leaf collection and pretreatment; (2) decolorization: soaking the leaves with 5% NaOH for 2 to 3 days; (3) leaf vein dyeing and color separation : According to the type of turfgrass, two methods are used to clear the veins: a. safranin dyeing and color separation dehydration; b safranin-fast green dyeing and color separation dehydration; After 24h (a) or 20-48h (b), you can watch and take images, and finally use software to analyze the density and percentage of leaf veins at all levels. The present invention improves multiple steps of blade pre-treatment, dyeing, color separation, dehydration, etc., and proposes a method for observing turfgrass vein network with simple operation, safety and environmental protection, short preparation cycle, good film production effect and clear image. Structural mount preparation method. It has universal adaptability to turfgrass, and can obtain clearer vein images more quickly, which can be used for related research on turfgrass vein network structure.

Description

technical field [0001] The invention belongs to the field of ecology and environmental protection, and relates to the analysis of the vein network structure of turfgrass, in particular to a method for preparing slices for observing the vein network structure of turfgrass. Background technique [0002] Turfgrass has a well-developed root system and high ground coverage. A large number of studies have shown that it plays a significant role in sewage purification and heavy metal absorption, and the lawn has both ornamental and sports performances. Therefore, it is in great demand in urban construction and in the construction of ecological civilization. Today, it is of great ecological significance to screen more excellent turfgrass varieties. In order to reduce evapotranspiration, turfgrass leaves mostly have the characteristics of low water content, rough texture, and waxy or hairy covering. Different turfgrass species have different leaf phenotype characteristics and water c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N21/84
CPCG01N1/28G01N1/30G01N21/84G01N2021/8466
Inventor 孙小玲宋重升柴旭旭宋午椰崔朝伟
Owner TIANJIN AGRICULTURE COLLEGE
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