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Repair membrane loaded with whole-cell active ingredients as well as preparation method and application of repair membrane

A technology of active ingredients and cell activity, which is applied in the field of repair membranes loaded with active ingredients of whole cells and its preparation, can solve the problems of cell activity instability, inability to fully exert and promote tissue regeneration, and prevent liver and kidney damage, fibrosis, etc. The effect of good morphology and mechanical properties and conventional equipment

Pending Publication Date: 2020-12-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure membrane materials cannot fully play the role of promoting tissue regeneration. Therefore, loading biologically active substances during the preparation of membrane materials and ensuring the biological activity of the loaded components can significantly improve the repair efficiency of membrane materials.
Cell functions are the most comprehensive and effective bioactive substances, but in vivo applications are limited by the instability of cell activity

Method used

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  • Repair membrane loaded with whole-cell active ingredients as well as preparation method and application of repair membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Degradable collagen membrane loaded with active components of mesenchymal stem cells and its preparation

[0017] 1) Preparation of whole-cell active ingredient micro-nano particles:

[0018] with PBS at 2 x 10 6 Cells were resuspended at a concentration of cells / mL; the suspended MSCs were sequentially extruded through polyester fiber membranes (Whatman) with pore sizes of 10 μm, 5 μm, 1 μm and 400 nm using a micro extruder (Avanti Polar Libers), 5 times for each scale , to prepare nanoparticles with a size of 400nm, carefully cover iododanol (10% (v / v)) on 50% (v / v) iododanol, and load the extruded suspension onto the top layer, 100000g Centrifuge for 2h; collect the solution layer between 50% and 10% iodanol, and ultracentrifuge again at 100,000g for 2h, and resuspend the centrifuged particles in PBS to obtain micro-nano bioactive particles;

[0019] 2) Preparation of membrane loaded with active ingredients of cells:

[0020] Dissolve the micro-nano bio...

Embodiment 2

[0021] Example 2: Degradable gelatin film loaded with active components of mesenchymal stem cells and its preparation

[0022] 1) Preparation of whole-cell active ingredient micro-nano particles:

[0023] with PBS at 5 x 10 6 Cells were resuspended at a concentration of cells / mL; the suspended MSCs were sequentially extruded through polyester fiber membranes (Whatman) with pore sizes of 10 μm, 5 μm, 1 μm and 400 nm using a micro extruder (Avanti Polar Libers), 5 times for each scale , to prepare nanoparticles with a size of 400nm, carefully cover iododanol (10% (v / v)) on 50% (v / v) iododanol, and load the extruded suspension onto the top layer, 100000g Centrifuge for 2h; collect the solution layer between 50% and 10% iodanol, and ultracentrifuge again at 100,000g for 2h, and resuspend the centrifuged particles in PBS to obtain micro-nano bioactive particles;

[0024] 2) Preparation of membrane loaded with active ingredients of cells:

[0025] The micro-nano bioactive particl...

Embodiment 3

[0026] Example 3: Degradable hyaluronic acid repair membrane loaded with micron-scale whole-cell active substances and its preparation

[0027] 1) Preparation of whole-cell active ingredient micro-nano particles:

[0028] 8 x 10 in PBS 6 The cells were resuspended at a concentration of cells / mL; the suspended MSCs were sequentially extruded through a polyester fiber membrane (Whatman) with a pore size of 10 μm, 5 μm and 1 μm using a micro-extruder (Avanti Polar Libers), 5 times for each scale, and prepared For micron particles with a size of 1 μm, carefully cover iodanol (10% (v / v)) on 50% (v / v) iodanol, load the extruded suspension onto the top layer, and centrifuge at 100000g for 2h ;Collect the solution layer between 50% and 10% iodanol, and ultracentrifuge again at 100,000g for 2h, and resuspend the centrifuged particles in PBS to obtain micro-nano bioactive particles;

[0029] 2) Preparation of membrane loaded with active ingredients of cells:

[0030] Dissolve micro-n...

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Abstract

The invention discloses a repair membrane loaded with whole-cell active ingredients as well as a preparation method and application of the repair membrane. According to the invention, active components of cells are fully utilized to directly perform micro-nano treatment on the cells, loading is performed by a physical or chemical method in the preparation process of the membrane material, and under the premise of ensuring the biological activity of the loaded components, the repair efficiency of the membrane material is remarkably improved. The preparation method comprises the steps of preparing a working solution and preparing the repair membrane loaded with the bioactive components. The repair membrane is used for medicine in-situ release, prolongs medicine action time and promotes tissue damage repair.

Description

technical field [0001] The field of the present invention is tissue engineering and regenerative medicine, specifically a repair membrane loaded with whole cell active components, its preparation method and application. [0002] technical background [0003] When repairing skin trauma, endometrial injury, and periodontal injury, the coverage of membrane materials is beneficial to wound repair and prevents bacterial infection, which is of great significance in the repair of large-scale damage. An ideal membrane material should have the following basic characteristics: (1) Good biocompatibility, the material itself and its degradation products have no toxic side effects on cells; (2) Degradability, the degradation rate matches the tissue regeneration rate; (3) Appropriate pore size, porosity and good penetration; (4) good mechanical properties, mechanical strength matching the implant site; (5) good stability, not easy to react with tissues. However, pure membrane materials ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/24A61L27/38A61L27/50A61L27/54
CPCA61L27/20A61L27/222A61L27/24A61L27/3834A61L27/50A61L27/54A61L2300/30A61L2300/412A61L2300/602C08L5/08
Inventor 齐春晓王淑芳孔德领董云生刘祥胜
Owner NANKAI UNIV
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