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Medicine for skin wounds and preparation method and application thereof

A drug and wound technology, applied in the field of skin wound drugs and its preparation, can solve the problems of hypertrophic scar healing, low activity, high cost, etc., and achieve the effect of repairing skin wounds

Pending Publication Date: 2020-12-08
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional wound healing drugs have certain limitations, such as high cost, relatively low activity and easy to cause hypertrophic scar healing

Method used

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  • Medicine for skin wounds and preparation method and application thereof
  • Medicine for skin wounds and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Preparation of hydrogel

[0025] According to the formula in Table 1 below, the prepared polypeptide was mixed with benzalkonium chloride, and then mixed with 100 mM CaCl 2 1. A PBS solution with a pH of 8.0 was prepared into a mixed solution, and the obtained mixed solution became a viscous hydrogel after being stirred.

[0026] Table 1: Hydrogel Proportions

[0027] Numbering Polypeptide (wt%) Benzalkonium Chloride (wt%%) Mass ratio of peptide to benzalkonium chloride 1 3％ 0 - 2 2.5％ 0.5％ 5:1 3 2.0％ 1％ 2:1 4 1.5％ 1.5％ 1:1 5 1.0％ 2.0％ 1:2

[0028] The prepared gel was subjected to frequency sweep rheology experiment at 25°C and 1% strain condition, the frequency sweep range was 1Hz-100Hz, and the experimental results were as follows: figure 1 shown. G represents the shear storage modulus, G' represents the shear loss modulus, and when G is significantly higher than G' in the entire scanning range,...

Embodiment 2

[0029] Embodiment 2: pharmacodynamic experiment

[0030] 1. Animal model preparation:

[0031] Hair removal: 5% pentobarbital sodium 4mg / 20g, anesthetized by intraperitoneal injection, cut off the long hair on the back, apply barium sulfide depilatory agent evenly on the back, remove the hair after a while, and wash the depilated area with water. After 24 hours, the same anesthesia was used to prepare the burn wound. A water bottle with a temperature of 90°C was lightly placed on the skin of the back of the mouse, and the scalding time was 15s. Then, the rats were reared in separate cages at a room temperature of 25°C, with free access to food and water. At 48 hours after injury, the appearance of wounds in rats was observed. At the same time, skin samples of wounds were taken, routinely fixed, stained with HE, and observed by light microscope histology to check whether the degree of epidermal burns at this time was consistent with deep second-degree burns.

[0032] 2. Wound...

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PUM

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Abstract

The invention relates to a medicine for skin wounds and a preparation method and application thereof. The medicine contains polypeptide and benzalkonium chloride, wherein the polypeptide has an aminoacid sequence shown as SEQ ID NO. 1 or an amino acid sequence which is deleted or replaced by any one amino acid or added with one amino acid compared with the sequence shown as SEQ ID NO. 1. The polypeptide and benzalkonium chloride contained in the medicine for skin wounds can form stable gel, and the synergistic effect of the polypeptide and benzalkonium chloride is beneficial to repairing skinwounds, especially second-level or above burns.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a medicine for skin wounds, a preparation method and application thereof. Background technique [0002] Skin wound repair has always been a difficult problem in the field of clinical medicine. The skin is the largest organ in the human body, serving as a physical barrier between the human body and the external environment, and it undertakes many important physiological functions, including perspiration, heat and pain sensation. Once the skin is damaged, it will cause an imbalance in the internal and external environment of the body, resulting in malnutrition, multiple organ failure and even death. Traditional wound healing drugs have certain limitations, such as high cost, relatively low activity and easy to cause hypertrophic scar healing. Therefore, it is very important and necessary to find new drugs for promoting wound repair. Compared with traditional woun...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61K9/06A61P17/02A61K31/14
CPCA61K38/08A61K31/14A61K9/06A61K9/0014A61P17/02
Inventor 夏卫东林才赵胜干添元
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV