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Penaeus monodon Pm GLUT2 gene and application thereof

A technology of penaeus monodon and gene, applied in the field of molecular biology, can solve the problems of insufficient metabolic function and lack of research.

Active Publication Date: 2020-12-08
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The salinity in the water environment will show obvious diurnal fluctuations and seasonality under natural conditions. In addition, severe weather such as heavy rains and typhoons and water changes in aquaculture production will also change the salinity in the water environment. This acute salinity However, the research on the response of Penaeus monodon to low-salt stress and the metabolic function under low-salt stress is not deep enough, especially the key transporter that plays a role in material transfer during the process of low-salt stress. research is still lacking

Method used

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  • Penaeus monodon Pm GLUT2 gene and application thereof
  • Penaeus monodon Pm GLUT2 gene and application thereof
  • Penaeus monodon Pm GLUT2 gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0046] 1. Experimental process

[0047] 1.1. Experimental animals

[0048] The monodon prawns were taken from the Shenzhen Experimental Base of the South China Sea Fisheries Research Institute. The prawns with a body length of 8±2cm and a body weight of 10±2g were selected to be temporarily raised in a cement pond (4.71m×4.13m×1.73m) in natural seawater. 5.84m 3 ), and fully aerated, the culture temperature is 27±2°C.

[0049] 1.2. Extraction of total RNA and construction of cDNA library

[0050] 3 healthy and complete male and female prawns were randomly selected from the temporarily raised prawns, and tissue samples such as hepatopancreas, gills, heart, intestine, stomach, lymph, epidermis, muscle, eye stalk nerve, ovary, and testis were collected from 3 The same kind of samples of tailed shrimp were mixed into one tube, stored in RNAlater at 4°C overnight, and then stored at -80°C for later use.

[0051] Total RNA was extracted from all samples according to the HiPure F...

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Abstract

The invention discloses a penaeus monodon Pm GLUT2 gene. The base sequence of the gene is as shown in SEQ ID NO: 2. The amino acid sequence of an encoded protein is shown as SEQ ID NO: 3. The invention also discloses application of the gene or the encoded protein of the gene in regulating the low-salt stress resistance of penaeus monodon. a screening method of a low-salt tolerance related SNP locus of the penaeus monodon Pm GLUT2 gene and application of the low-salt tolerance related SNP locus, screened by the method, of the penaeus monodon in penaeus monodon breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a PmGLUT2 gene of Penaeus monodon and its application. Background technique [0002] Glucose transporter-2 (GLUT2, glucose transporter 2) belongs to the glucose transporter family (GLUTs), which was first discovered from human liver and kidney, and it is specifically expressed in liver, kidney, and small intestine, and later found in pancreatic islet β It is also highly specifically expressed in cells. The low affinity and high saturation for glucose are the most prominent features of GLUT2, which is mainly distributed in the basement membrane of epithelial cells in the liver, kidney, small intestine and pancreatic islet B cells, and participates in the absorption and transport of glucose. At present, most studies on GLUT2 and sodium-glucose co-transporter are related to mammalian diseases, and there are few studies on aquatic animals. Terova et al. studie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12N15/12C12N15/10C12Q1/6888
CPCC07K14/43509C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李运东周发林江世贵何鹏
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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