A kind of pm GLUT2 gene of Penaeus monodon and its application

A technology of penaeus monodon and gene, applied in the field of molecular biology, can solve the problems of insufficient metabolic function and lack of research.

Active Publication Date: 2021-08-20
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The salinity in the water environment will show obvious diurnal fluctuations and seasonality under natural conditions. In addition, severe weather such as heavy rains and typhoons and water changes in aquaculture production will also change the salinity in the water environment. This acute salinity However, the research on the response of Penaeus monodon to low-salt stress and the metabolic function under low-salt stress is not deep enough, especially the key transporter that plays a role in material transfer during the process of low-salt stress. research is still lacking

Method used

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  • A kind of pm GLUT2 gene of Penaeus monodon and its application
  • A kind of pm GLUT2 gene of Penaeus monodon and its application
  • A kind of pm GLUT2 gene of Penaeus monodon and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] First, the experiment process

[0047] 1.1, experimental animals

[0048] Spotstone Shrimp Since the Shenzhen Water Research Institute Shenzhen Test Base, the body length is 8 ± 2cm, the body mass is 10 ± 2G, which is temporarily placed on the cement pool of natural seawater (4.71m × 4.13m × 1.73m, aquaculture water body volume 5.84m 3 And sufficiently aerated, the culture temperature is 27 ± 2 ° C.

[0049] 1.2, total RNA extraction and construction of cDNA libraries

[0050] From the trampires from the shrimp, select a healthy and complete male shrimp 3, take the liver pancreatic, sputum, heart, intestinal, stomach, lymph, epidermus, muscle, eye shank, ovary, and fine nest, from 3 The same sample of the tail shrimp is mixed into a tube, and it is stored at -80 ° C preservation in the RNALATER 4 ° C.

[0051] The total RNA of all samples was extracted according to the method of the Hipure Fibrous RNA Plus Kit kit. 10 × Loading Buffer and RNA mixed with RNA, the nucleic aci...

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Abstract

The invention discloses a Pm GLUT2 gene of Penaeus monodon, the base sequence of which is shown in SEQ ID NO:2. The amino acid sequence of the encoded protein is shown in SEQ ID NO:3. Also disclosed is the application of the above-mentioned gene or the encoded protein of the gene in regulating the low-salt stress resistance of Penaeus monodon. And a method for screening SNP loci related to low-salt resistance of Pm GLUT2 gene of P. monodon and the application of the SNP loci related to low-salt resistance of P. monodon screened by the method in breeding of P. monodon.

Description

Technical field [0001] The present invention belongs to the field of molecular biology, and in particular, the present invention relates to a pm pmglut2 gene and its application thereof. Background technique [0002] Glucose transporter-2 (Glut2, Glucose Transporter 2) belongs to the glucose transporter family (gluts), the earliest from human liver and kidneys, in the liver, kidney, small intestines, and later found in islet beta The cells are also specific in cells. The low affinity and high saturation of glucose is the most significant feature of GLUT2, which is mainly distributed over the liver, kidney, small intestine and islet B cells, and participate in completion of glucose absorption and transportation. Currently, the study of GLUT2 and the sodium-glucose total transportation with mammalian diseases are less studied in aquatic animal. Terova et al. The function of Glut2 in the European Wolf, indicating that Glut2 may play out the function of glucose transfer formed by liv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C12N15/12C12N15/10C12Q1/6888
CPCC07K14/43509C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李运东周发林江世贵何鹏
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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