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A kind of method for fermenting and producing acarbose

A technology of fermentation culture and fermentation medium, applied in the field of fermentation and production of acarbose, which can solve the problems of unstable yield, low yield, and long cycle, and achieve the effects of stable yield, short fermentation cycle, and high fermentation yield

Active Publication Date: 2022-07-15
SHANDONG LUKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Acarbose can be produced by microbial fermentation, but there are problems of long cycle, low yield and unstable yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Wash the mobile actinomycetes on the 4 slants with 10 mL of sterile water to prepare a bacterial suspension, connect the bacterial suspension to a 1000 mL conical flask with a seed medium filling capacity of 20%, and shake at 28°C with a shaker (120 rpm). ) culture for 48h; inoculate the shake flask seed liquid (3000mL) into the fermentation medium with the capacity of 20L / 30L tank, the culture conditions are the tank temperature of 28°C and the tank pressure of 0.04 to 0.05MPa, through the adjustment of stirring speed and ventilation , the dissolved oxygen in the fermentation process is controlled at 32%. During the fermentation process, the mixed solution of glucose and potassium dihydrogen phosphate is added to the flow for 40 to 100 hours, and the glucose content of the fermentation broth is controlled to be between 10 to 20 g / L. After 160 hours of fermentation, the fermentation is completed. The residual glucose in the liquid was controlled within 10g / L, and the aca...

Embodiment 2

[0029] Wash the bacteria on the 4 slants with 10 mL of sterile water to prepare a bacterial suspension, connect the bacterial suspension to a 1000 mL conical flask with a seed medium content of 20%, and cultivate at 28°C with a shaker (120 rpm) for 48 hours. The shake flask seed liquid (3000mL) is inoculated into the fermentation medium of the 20L / 30L tank, and the culture condition is that the tank temperature is 28 ° C, the tank pressure is 0.04~0.05MPa, and the fermentation is carried out by the adjustment of the stirring speed and the ventilation rate. The dissolved oxygen in the process is controlled at 28%. During the fermentation process, the mixed solution of glucose and potassium dihydrogen phosphate is added in 40-100 hours, and the glucose content of the fermentation broth is controlled to be between 10-20 g / L. After 165 hours of fermentation, the remaining glucose in the fermentation broth Controlled within 10g / L, the acarbose content was 7400mg / L.

[0030] Seed me...

Embodiment 3

[0034] Wash the bacteria on the 4 slants with 10 mL of sterile water to prepare a bacterial suspension, connect the bacterial suspension to a 1000 mL conical flask with a seed medium content of 20%, and cultivate at 28°C with a shaker (120 rpm) for 48 hours. The shake flask seed liquid (3000mL) is inoculated into the fermentation medium of the 20L / 30L tank, and the culture condition is that the tank temperature is 28 ° C, the tank pressure is 0.04~0.05MPa, and the fermentation is carried out by the adjustment of the stirring speed and the ventilation rate. The dissolved oxygen in the process is controlled at 30%. During the fermentation process, the mixed solution of glucose and potassium dihydrogen phosphate is added in 40h-100h, and the glucose content of the fermentation broth is controlled to be between 10 and 20g / L. After 162h of fermentation, the remaining glucose in the fermentation broth Controlled within 10g / L, the acarbose content was 7880mg / L.

[0035] Seed medium f...

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Abstract

The invention provides a method for producing acarbose by fermentation, which belongs to the technical field of fermentation. The method comprises the following steps: 1) inoculating a strain of Actinomyces mobilis into a seed medium for seed culture to obtain seed liquid; 2) ) Inoculate the seed liquid into a fermentation medium for 160-170 h of fermentation to obtain a fermentation liquid; the temperature of the fermentation is 27-29°C, and the pressure of the fermentation is 0.04-0.05MPa; the fermentation is The dissolved oxygen in the fermentation system is 25% to 35%; during the 40 to 100h period of the fermentation culture, the mixed solution of glucose and potassium dihydrogen phosphate is added to the fermentation system, and the glucose content in the fermentation system is controlled to be within 10 to 20 g / L. between. The method of the invention has high yield, short cycle and stable yield.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing acarbose by fermentation. Background technique [0002] Acarbose, an α-glucosidase inhibitor, is a complex oligosaccharide, similar in structure to oligosaccharides, which can reversibly bind to α-glucosidase and inhibit various α-glucosidase such as The activity of maltase, isomaltase, glucoamylase and sucrase decomposes starch into oligosaccharides such as maltose (disaccharide), maltotriose and dextrin (oligosaccharide) and then slows down the rate of decomposition into glucose. The decomposition of sucrose into glucose and fructose slows down, thus slowing the absorption of intestinal glucose, thereby alleviating postprandial hyperglycemia and reducing blood sugar. If acarbose is taken for a long time, it can reduce the concentration of fasting blood sugar and glycated hemoglobin. [0003] Acarbose can be produced by microbial ferment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12N1/20C12R1/045
CPCC12P19/26C12N1/20
Inventor 史磊杨素娟朱维忠郑长春张开芬
Owner SHANDONG LUKANG PHARMA
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