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High-throughput bioactive cell imaging screening system

A technology of cell imaging and biological activity, applied in the direction of biomass post-treatment, biomass pretreatment, biochemical cleaning equipment, etc., can solve the problems of operator fatigue, single steps, and boring work, so as to avoid impact and improve sensitivity High, efficiency-enhancing effect

Pending Publication Date: 2020-12-08
江苏拓开生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In high-throughput screening, tens of millions of samples must be tested every day. The work is boring and the steps are single. Operators are prone to fatigue and make mistakes.

Method used

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  • High-throughput bioactive cell imaging screening system
  • High-throughput bioactive cell imaging screening system
  • High-throughput bioactive cell imaging screening system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] refer to Figure 1-3 , a high-throughput biologically active cell imaging and screening system, including an intelligent microscope stage 1, an imaging component, a high-throughput screening component and a controller 15; the interior of the intelligent microscope stage 1 is equipped with a rotating mechanism, and the rotating mechanism is controlled by a servo Driven by the motor 4, the output shaft of the servo motor 4 drives the object tray 6 to rotate through the drive shaft 5, and the object tray 6 drives multiple cell culture plates 3 to quickly enter the imaging area below the imaging assembly in sequence.

[0031] The imaging component is composed of an inverted fluorescence microscope 19 and a high-speed CCD camera 20. The inverted fluorescence microscope 19 is installed above the intelligent microscope stage 1 through the elevator platform 9. The high-speed CCD camera 20 is fixedly installed above the elevator platform 9 and is located on the inverted fluoresce...

Embodiment 2

[0037] refer to Figure 4-5 , different from Embodiment 1, the elevator table 9 is composed of a mounting frame 17 and an electric telescopic rod 18, the electric telescopic rod 18 is coupled with the controller 15, and the upper surface of the loading tray 6 is provided with a plurality of detection slots 7, each An electromagnet block 8 is embedded in the inner wall of the detection groove 7 .

[0038] The material of the cell culture plate 3 is tempered glass, and the lower surface of the cell culture plate 3 is fixedly connected with an iron block. After the electromagnet block 8 is energized, it generates magnetic adsorption with the iron block. Connected battery.

[0039] In this embodiment, the cell culture plate 3 can be quickly fixed and disassembled by setting the electromagnet block 8, which provides convenience for replacing the next batch of detection samples, thereby facilitating the development of imaging screening work.

[0040] Working principle: firstly, th...

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Abstract

The invention discloses a high-throughput bioactive cell imaging and screening system which comprises an intelligent microscope table, an imaging assembly and a high-throughput screening assembly. Theimaging assembly is in communication connection with the high-throughput screening assembly, the high-throughput screening assembly is a PC desktop computer, a rotating mechanism is arranged in the intelligent microscope table, the rotating mechanism is used for driving a cell culture plate to move into and out of the imaging area of the imaging assembly, and an automatic rotating mechanism in the intelligent microscopic table comprises a servo motor fixedly connected to the inner bottom wall of the intelligent microscopic table. According to the system, a servo motor is adopted to drive a cell culture plate to enter an imaging area below an inverted fluorescence microscope, a high-speed CCD camera shoots a picture displayed after amplification of the inverted fluorescence microscope andtransmits the picture to the PC desktop computer for high-throughput screening by using software, and the servo motor realizes automatic switching of the cell culture plate, so that the efficiency isimproved, the time interval between cell samples in the same batch is shortened, the influence on active cells due to overlong time is avoided, and the detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of cell imaging screening, in particular to a high-throughput bioactive cell imaging screening system. Background technique [0002] In high-throughput screening, tens of millions of samples are tested every day. The work is boring and the steps are single. Operators are prone to fatigue and mistakes. The automatic operating system consists of four parts: computer and its operating software, automatic sampling equipment, incubation and centrifugation equipment and stack. The automated operating system obviously has many advantages in replacing manual operations. It uses computers to control the entire experimental process through operating software, and the programming process is simple and clear. [0003] At present, most of the screening for bioactive cell imaging adopts high-throughput screening technology. [0004] In the overall screening system, the replacement of biological samples and the displacem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N21/84G01N21/01C12M3/00C12M1/34C12M1/38
CPCG01N15/10G01N21/84G01N21/01C12M41/36C12M41/12G01N2015/1006G01N2015/1022
Inventor 陈倩
Owner 江苏拓开生物科技有限公司