Polygonatum kingianum homogeneous polysaccharide and preparation method and application thereof

Abstract

The invention discloses polygonatum kingianum homogeneous polysaccharide and a preparation method and application thereof, the total sugar content of the polygonatum kingianum homogeneous polysaccharide is more than 95%, and the molecular weight of the homogeneous polysaccharide is 1-70kDa. The method for preparing the polygonatum kingianum homogeneous polysaccharide comprises the following steps:taking dried polygonatum kingianum, performing degreasing, extracting, precipitating and drying to obtain a polygonatum kingianum polysaccharide crude product; dissolving the obtained polygonatum sibiricum polysaccharide crude product with distilled water, loading the solution to an anionic cellulose chromatographic column, and performing eluting with distilled water to obtain polygonatum sibiricum washing component polysaccharide; purifying the obtained washing component polysaccharide by using a gel chromatographic column, and performing eluting by using a NaCl solution to obtain the polygonatum kingianum homogeneous polysaccharide. The polygonatum kingianum homogeneous polysaccharide is applied to an in-vitro antioxidant process. According to the invention, polygonatum sibiricum homogeneous polysaccharide is extracted and separated from polygonatum sibiricum for the first time and is subjected to carbohydrate chain sequencing. The preparation method is simple, high-purity homogeneous polysaccharide can be rapidly obtained, and the preparation method is suitable for large-scale production.

Description

technical field

[0001] The invention relates to the technical field of Polygonatum application, in particular to Polygonatum homogeneous polysaccharide, its preparation method and application. Background technique

[0002] Polygonatum sibiricum (Polygonatum sibiricum) is the dried rhizome of Polygonatum sibiricum, a traditional Chinese medicine in my country, which has the effects of invigorating qi and nourishing yin, strengthening the spleen, moistening the lungs and kidneys. There are many varieties of Polygonatum included in the Chinese Pharmacopoeia, mainly including Polygonatum, Polygonatum yunnanensis and Polygonatum multiflora. Also known as chicken claw ginseng, yellow chicken dish, chicken head sealwort, tiger ginger, pen tube dish. Polygonatum polysaccharide is the rhizome of Polygonatum dried, extracted with hot water and precipitated with ethanol to obtain Polygonatum crude polysaccharide. Polygonatum polysaccharide (PSP) has good biological activity, PSP can ...

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