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Myasthenia gravis diagnostic reagent kit taking microorganisms as diagnostic markers and application

A myasthenia gravis and microbial technology, applied in the biological field, can solve the problems of treatment delay, easy misdiagnosis as oculomotor nerve paralysis, etc., and achieve the effect of high sensitivity

Active Publication Date: 2020-12-11
SHIJIAZHUANG PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the symptoms of early muscle weakness are not specific, it is often misdiagnosed as oculomotor nerve palsy, vertical gaze palsy, or motor neuron disease, which causes delay in treatment (Querol L, Illa I. Myasthenia Gravis and the Neuromuscular Junction. Curr Opin Neurol .2013; 5:459-65; Liewluck T. Immune-Mediated Rippling Muscle Disease: Another Inflammatory Myopathy in Myasthenia Gravis. Arch Neurol. 2010; 7:896-7,897.)

Method used

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  • Myasthenia gravis diagnostic reagent kit taking microorganisms as diagnostic markers and application
  • Myasthenia gravis diagnostic reagent kit taking microorganisms as diagnostic markers and application
  • Myasthenia gravis diagnostic reagent kit taking microorganisms as diagnostic markers and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Screening of intestinal flora associated with myasthenia gravis

[0054] 1. Research objects and sample collection

[0055] 55 cases of children with myasthenia gravis and 36 healthy controls (HC) of corresponding age and sex were collected in the Myasthenia Gravis Treatment Center of the First Hospital of Shijiazhuang City, Hebei Province. The sample information is shown in Table 1.

[0056] Diagnostic criteria: (1) clinical manifestations: ptosis, diplopia, strabismus; (2) neostigmine test positive: (3) acetylcholine receptor antibody antibody positive: (4) electromyography: facial nerve attenuation at low frequency, high frequency No increment. Those who meet one of (1)+(2) or (3) or (4) can be diagnosed clearly.

[0057] Type: Refer to the new clinical type and quantitative myasthenia gravis score (QMG) standard type proposed by the Myasthenia Gravis Association of America (MGFA) in 2000.

[0058] Inclusion criteria: The patients were clearly diagnosed...

Embodiment 2

[0085] Example 2 Verifies the accuracy of genome sequencing

[0086] According to the method of Example 1, 19 samples of myasthenia gravis and 13 samples of healthy people were collected, and the patient information is shown in Table 4.

[0087] Table 4 Sample clinical characteristics

[0088]

[0089] Differential flora Prevotella_copri, Clostridium_bartlettii, Fusobacterium_mortiferum, and Helicobacter_cinaedi were randomly selected for sequencing verification, and their diagnostic efficacy for myasthenia gravis was calculated.

[0090] The results showed that the AUC values ​​of Prevotella_copri, Clostridium_bartlettii, Fusobacterium_mortiferum, and Helicobacter_cinaedi were 0.736842105, 0.672064777, 0.821862348, and 0.615384615, respectively, which were comparable to the previous detection results, indicating that the sequencing data of the metagenomic group was accurate.

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Abstract

The invention discloses a myasthenia gravis diagnostic reagent kit taking microorganisms as diagnostic markers and an application. The reagent kit comprises a reagent capable of detecting the microbial markers, and the microorganisms are Prevotella_stercorea or a combination of the Prevotella_stercorea and Sutterella_parvirubra. The myasthenia gravis can be predicted, and a rapid diagnostic reagent for preventing and diagnosing myasthenia gravis is developed.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a diagnostic kit and application of a myasthenia gravis using microorganisms as diagnostic markers. Background technique [0002] The main symptom of myasthenia gravis is that the production of autoantibodies affects the transmission of neuromuscular junction transmitters, which in turn causes the fatigue of striated muscles, which is manifested as lightness in the morning and evening weight, and aggravation after activity or fatigue, resulting in fluctuations in symptoms. The levator muscle and extraocular muscles are usually most easily involved, so the first symptoms of the disease are usually drooping eyelids and diplopia, and 20% of patients' symptoms may be limited to the weakness and asymmetry of the extraocular muscles. In addition, symptoms can gradually involve the medulla oblongata and limbs, and limb weakness is usually symmetrical, with more proximal symptoms than distal s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/689G01N33/569G16B30/00G16B5/00A61K45/00A61P21/04C12R1/01
CPCC12Q1/6883C12Q1/689G01N33/56911G16B30/00G16B5/00A61K45/00A61P21/04G01N2800/10Y02A50/30
Inventor 乞国艳
Owner SHIJIAZHUANG PEOPLES HOSPITAL
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