Method for rapidly determining content of total glycosides in Chinese herbal compound
A technology for rapid determination of total sugar content, applied in measuring devices, preparation of test samples, measurement of color/spectral characteristics, etc., can solve the problems of poor universality, difficulty in obtaining, and high price of standard products, and achieve mature and easy-to-use methods. Operation, the effect of improving the extraction rate
Pending Publication Date: 2020-12-11
HUNAN UNIV OF CHINESE MEDICINE
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0006] (2) The universality of the method is poor
Because there are many types of glycosides with different characteristics, and some glycosides such as ginsenosides have weak UV response, it is difficult to detect the content of all glycosides in the total glycosides at the same time by the current UV spectrophotometry or high performance liquid chromatography
[0007] (3) Standard products are expensive and difficult to obtain
Existing determination methods require the purchase of one or more standard products, but some standard products are expensive and difficult to obtain, which also limits the accuracy of content determination
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0035] The total glycosides in the traditional Chinese medicine compound "Qiweibaizhusan" were used as the determination object. Qiwei Baizhu San comes from "Children's Medicine and Syndrome Zhijue", which is composed of ginseng, white poria cocos, fried Atractylodes macrocephala, Huoxiang, woody fragrance, licorice and kudzu root. From the perspective of the drugs in the prescription, the total glycosides of Qiweibaizhusan include ginsenoside, glycyrrhizic acid, liquiritin, formononetin, puerarin, daidzin, genistin, hyperin, etc., mainly flavonoid glycosides and Saponins, wherein puerarin is a flavonoid carbon glycoside.
[0036] The structure of flavone carbon glycosides is relatively stable, and it needs to be acid-hydrolyzed under conditions of strong acid, high temperature or high pressure. For example, flavone carbon The acid hydrolysis condition of glycosides is the acid hydrolysis standard of the total glycosides of the traditional Chinese medicine compound, and the a...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a method for rapidly determining the content of total glycosides in a traditional Chinese medicine compound, which comprises the following steps of S1, extraction: taking a traditional Chinese medicine compound composition, and extracting to obtain an extracting solution which is a traditional Chinese medicine compound extract, S2, separation and purification: carrying out separation and purification on the traditional Chinese medicine compound extract to obtain traditional Chinese medicine compound total glycosides, and S3, measurement of thecontent of total glycosides:measuring the content of reducing sugar in the traditional Chinese medicine compound total glycosides through a DNS method, then conducting acidolysis on the traditional Chinese medicine compound total glycosides to obtain total sugar, measuring the content of the total sugar through the DNS method, and representing the content of the traditional Chinese medicine compound total glycosides throughthe result obtained by subtracting the content of the reducing sugar from the content of the total sugar. The method provided by the invention does not need to purchase standard substances of glycoside compounds, does not need to consider measuring instruments and conditions in combination with the types of the glycoside compounds; and the method of measuring the reducing sugar and the total sugar by DNS is mature, simple, convenient and rapid, does not need expensive instruments, is easy to operate, and is suitable for optimizing a total glycoside extraction process in the traditional Chinese medicine compound.
Description
technical field [0001] The invention relates to the technical field of quality inspection of traditional Chinese medicine compound prescriptions, and more specifically, relates to a method for rapidly determining the content of total glycosides in traditional Chinese medicine compound prescriptions. Background technique [0002] Glycosides are widely found in traditional Chinese medicine and are also active ingredients of many traditional Chinese medicines. A large number of pharmacological studies have confirmed that glycosides exhibit important pharmacological activities on the cardiovascular system, respiratory system, digestive system, nervous system, and immune system. For example, ginsenosides have activities such as anti-tumor, anti-fatigue, anti-aging, enhancing immunity and improving memory. Puerarin and daidzein in kudzu root have pharmacological effects such as anti-oxidation, cardiovascular protection, anti-cancer, anti-inflammation, anti-ulcer and estrogen. Th...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N21/31G01N21/78G01N1/28
CPCG01N21/31G01N21/78G01N1/28G01N2021/786G01N2021/775
Inventor 谢果珍唐圆肖嫩群谭周进
Owner HUNAN UNIV OF CHINESE MEDICINE