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Extraction process and application of water extract of new medicinal part of polygonatum sibiricum

A technology of extraction temperature and extraction time, applied in the field of new medicinal parts of botanical medicinal materials, which can solve the problems of single evaluation index and the like

Pending Publication Date: 2022-03-25
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the shortage of single evaluation index in the prior art and the actual demand, the object of the present invention is to provide a kind of optimization and application of the extraction process of the stem and leaf water extract of Polygonatum Polygonatum. The extraction method is simple and fast, which solves the problem of Stems and leaves are discarded as waste, and their application in anti-oxidation and hypoglycemia is studied, providing a research basis for finding natural anti-oxidation and hypoglycemic drugs

Method used

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  • Extraction process and application of water extract of new medicinal part of polygonatum sibiricum
  • Extraction process and application of water extract of new medicinal part of polygonatum sibiricum
  • Extraction process and application of water extract of new medicinal part of polygonatum sibiricum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 Determination of chemical composition content

[0019] 1. This embodiment provides a method for the determination of polysaccharide content

[0020] Anthrone-sulfuric acid method was used to determine the content of polysaccharides. Precisely pipette 2.0ml of the sample into a clean test tube, put it in an ice-water bath, add 8 ml of anthrone-sulfuric acid, boil in water for 8 minutes, and then take an ice-water bath for 10 minutes to room temperature. Absorbance was measured at 582 nm. And substitute into the glucose standard curve y=36.463x-0.0107, R 2 =0.9992, the concentration showed a good linear relationship between 20.0-120.0 μg / ml.

[0021] 2. The present embodiment provides a method for the determination of saponin content

[0022] Vanillin-glacial acetic acid method was used to determine the content of saponins. Take 0.10 ml sample in a clean test tube, add 0.2 ml vanillin-glacial acetic acid and 0.8 ml concentrated sulfuric acid, bathe in wate...

Embodiment 2

[0025] Example 2 Extraction process optimization

[0026] 1. Single factor experiment

[0027] The absorbance of polysaccharides, saponins and pigments in water extracts of Rhizoma Polygonatum stems and leaves was determined by selecting extraction times, extraction temperature, extraction time and ratio of solid to liquid.

[0028] (1) The extraction temperature was fixed at 80°C, the extraction time was 2 h, and the ratio of solid to liquid was 1:20. The number of extractions was changed for 1, 2, and 3 times, and the absorbance of polysaccharides, saponins, and pigments were measured respectively. The experimental results are shown in the appendix figure 1 As shown, when the number of extractions is 2 times, the absorbance of polysaccharides and saponins is higher, the content is higher, and the absorbance of pigments is lower than that of 3 times of extraction, so considering the number of times of extraction, 2 times is the best choice.

[0029] (2) The extraction times ...

Embodiment 3

[0065] Example 3 Study on the in vitro activity of the crude extracts of Polygonatum stems, leaves and rhizomes

[0066] 1. In vitro anti-oxidation

[0067] 1.1 Determination of DPPH free radical scavenging activity

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Abstract

The invention discloses an optimized polygonatum sibiricum stem and leaf water extract extraction process and application thereof. A single-factor experiment is carried out on a traditional hot water reflux method, the influence of the change of the material-liquid ratio, the extraction temperature, the extraction time and the extraction times on main components of stems and leaves is researched, a response surface experiment design is combined with an analytic hierarchy process, and four-factor three-level experiment design is carried out. And optimizing by taking the comprehensive score of the contents of polysaccharide, saponin and pigment as an inspection index to obtain the optimal extraction process of the stem and leaf water extract. And the biological activity of the stem leaf and rhizome crude extract is compared. The invention proves that the antioxidant activity and the hypoglycemic activity of the stem and leaf part are superior to those of the rhizome part, provides a novel extraction optimization technology for preparing the water extract, researches the application of the water extract in antioxidant activity and hypoglycemic activity, and provides a theoretical basis for further development of polygonatum sibiricum stems and leaves.

Description

technical field [0001] The invention relates to a new medicinal part of a plant medicinal material, the optimization and application of an extraction process of stem and leaf water extract of Polygonatum polygonatum, and belongs to the technical field of extraction and application of natural products. Background technique [0002] Polygonatum sibiricum Red belongs to Liliaceae. It is a perennial herb and one of the homologous plants of medicine and food. It is used as medicine with rhizome. It is sweet in taste and flat in nature. It has the effects of lowering blood sugar and blood pressure, and long-term use can improve people's physical fitness. It is a commonly used traditional Chinese medicine in clinical practice. According to its shape and size, it is divided into three categories: "ginger-shaped sealwort", "chicken head sealwort" and "rhubarb essence". Generally, the parts that are processed and utilized are underground tubers, and the stems and leaves of the aboveg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969A61P39/06A61P3/10A61K127/00A61K135/00
CPCA61K36/8969A61P39/06A61P3/10A61K2236/331A61K2236/39
Inventor 周鸿立单佳乐周欣鹤付梦霞张杰
Owner JILIN INST OF CHEM TECH