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Method for quickly separating polar components in frying oil on line

A polar component and frying oil technology, applied in the detection field, can solve the problems of sample loss, high cost, cumbersome steps, etc., and achieve the effect of high accuracy, low cost and fast separation speed

Active Publication Date: 2020-12-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method first uses silica gel column chromatography to obtain the total polar substances, and then uses high performance liquid chromatography (normal phase silica gel column) to further use polarity to separate each polar component, which has the disadvantages of cumbersome steps, high cost, and sample loss.

Method used

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  • Method for quickly separating polar components in frying oil on line
  • Method for quickly separating polar components in frying oil on line
  • Method for quickly separating polar components in frying oil on line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: A device for quickly separating polar components in frying oil online

[0033] Such as figure 1 Said, the device is composed of a gel chromatography column, a differential refraction detector and a silica gel chromatography column connected in series, the gel chromatography column, the differential refraction detector, The silica gel chromatography columns are connected in series, and the outlet of the gel chromatography column, the inlet of the differential refraction detector and the outlet of the silica gel chromatography column are connected to the same multi-way valve; the outlet of the differential refraction detector and the inlet of the silica gel chromatography column pass through at least two The multi-way valve is connected; the multi-way valve connected to the outlet of the differential refraction detector is connected with a nitrogen interface; the multi-way valve connected to the inlet of the silica gel chromatography column is connected with an...

Embodiment 2

[0035] Embodiment 2: A kind of method for rapidly separating polar components in frying oil

[0036](a) Accurately weigh 200 mg of frying oil stored at -20°C, dissolve it in a little dichloromethane and load it on a gel chromatography column (Sephadex LH-20 filler), add eluent (methanol: dichloromethane Methane=2:3) separation. Such as figure 1 As described in a, the gel chromatography column and the differential refractive index detector are connected in series through valve 1. The gel column chromatography first separates the components with large molecular weight differences, and then enters the differential refractive index detector through valve 1 for detection to obtain TGO. , TGD, the flow rate of the gel column is one drop per second. From the 10th minute, the polar substances will flow out separately. The first flow out is TGO (10-15min), followed by TGD (15-19.5min).

[0037] (b) if figure 1 As described in b, after TGD is collected, switch valve 2 so that TG and ...

Embodiment 3

[0040] Embodiment 3: A kind of method for detecting polar component content in frying oil

[0041] Adopt the method for embodiment 2 to separate the polar substance in the frying oil, obtain six kinds of components, detect its content through differential refraction detector, concrete steps are as follows:

[0042] (a) Accurately weigh 0.0100 g of the six components obtained in Example 2 and dissolve them in 1.0 mL of tetrahydrofuran, and then configure them to have concentrations of 0.01 g / mL, 0.008 g / mL, 0.006 g / mL, and 0.004 g / mL. mL, 0.002g / mL, 0g / mL standard solution (or 0.01g / mL, 0.0075g / mL, 0.005g / mL, 0.0025g / mL, 0g / mL), each component can be obtained by differential refractive index detector The standard curve as Figure 5 As shown, R 2 All are greater than 0.99; the differential refractive index detector is in the standard mode; the allowable value of drift is 5000nRIU / h; the allowable value of noise is 500nRIU; the temperature is 35°C; the flow rate is 2.4mL / min. ...

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Abstract

The invention discloses a method for rapidly separating polar components in frying oil on line, and belongs to the technical field of detection. The gel chromatographic column, the differential refraction detector and the silica gel chromatographic column are connected in series through the four-way valve and the two six-way valves, firstly, all components in the frying oil are separated by utilizing the characteristic that the gel chromatographic column separates substances with different molecular weights through the molecular weights, and six components including TGO, TGD, an ox-TG and TG mixture, DG, FFA and a degradation product can be rapidly separated. the ox-TG and TG mixture with small molecular weight difference and large polarity difference is collected into a collector by controlling a six-way valve; and finally, the mixture is separated through a silica gel chromatographic column by utilizing polarity difference to obtain an ox-TG component. According to the method, the gel chromatographic column, the differential refraction detector and the silica gel chromatographic column are connected in series, so that polar components in the frying oil are completely separated, and the method has the advantages of online monitoring, simplicity in operation, low cost and high speed.

Description

technical field [0001] The invention relates to an online rapid separation method for polar components in frying oil, belonging to the technical field of detection. Background technique [0002] Frying is a traditional cooking method in our country. The various flavor substances produced during the frying process make fried food very popular. However, triglycerides (TG) rich in fats are prone to a series of oxidation, polymerization, hydrolysis, and cracking reactions in the presence of high temperature, oxygen, and moisture to produce oxidized triglycerides (ox-TG), triglycerides Dimers (TGD), triglyceride oligomers (TGO), diglycerides (DG), degradation products and other substances with higher polarity than TG are collectively referred to as polar substances. Polar substances have been proven to be harmful to human health, and the most harmful component is ox–TG. However, the current discarding standard for frying oil is based on the total polar substance (TPC) content in...

Claims

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Application Information

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IPC IPC(8): G01N30/08G01N30/74
CPCG01N30/08G01N30/74
Inventor 刘元法曹鑫宇徐勇将李雪袁立阳翟颖红郑召君
Owner JIANGNAN UNIV
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