Blank multivesicular liposome as well as preparation method and device thereof

A multivesicular liposome and blank technology, which is applied in the fields of botanical equipment and methods, chemical instruments and methods, animal repellents, etc., can solve the problem of loose multivesicular liposome structure, uneven vesicle size, Poor vesicle stability and other problems, to achieve the effect of improving drug release time, good vesicle stability, and compact structure

Inactive Publication Date: 2020-12-25
YANGZHOU SPED CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at many problems such as the loose structure of the multivesicular liposome obtained by the existing preparation method, poor vesicle stability, excessively large and uneven particle size, etc., the present invention provides a preparation method of a blank multivesicular liposome, which utilizes continuous An environment-friendly multivesicular liposome with good vesicle stability and uniform particle size distribution was obtained by flow microchannel reactor;
[0008] In view of the traditional high-speed shearing equipment used in the preparation process of existing multivesicular liposomes, resulting in problems such as loose multivesicular liposome structure, leakage, excessive particle size and uneven particle size, the present invention provides a blank multi-vesicle liposome The preparation device of vesicular liposomes uses a continuous flow microchannel reactor to replace traditional high-speed shear equipment to improve the vesicle stability and particle size uniformity of multivesicular liposomes and reduce the vesicle size;
[0009] In view of the problems that the existing multivesicular liposome vesicles are not uniform in size, need to be processed again before use, and the size of the vesicles is too large to penetrate the target waxy layer, pores, etc., which are not suitable for the field of pesticide preparations. The invention provides an environment-friendly multivesicular liposome with good vesicle stability, uniform and moderate particle size distribution, and capable of encapsulating pesticide active substances for use in the field of pesticides

Method used

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  • Blank multivesicular liposome as well as preparation method and device thereof
  • Blank multivesicular liposome as well as preparation method and device thereof
  • Blank multivesicular liposome as well as preparation method and device thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076]The preparation device of the multivesicular liposome in this embodiment is asfigure 1Shown. Among them, the treatment kettle A 111 and the treatment kettle B121 are 2L reaction kettles, and the treatment kettle C 131 is a 15L conventional small-scale reaction kettle; the number n of the micro-reaction group 210 is 5; among them, the thermostatic tube (A 114, B 124, C 134) has a length of 0.3m and a diameter of 3mm; the delay tube 213 has a diameter of 3mm and a length of 1m; the microchannel cross-sectional equivalent diameter of the microchannel reactor 211 is 0.5mm, and the microchannel length is 1m; The mixer 212 has a plurality of microchannels, the cross-sectional equivalent diameter of the microchannels is 0.3mm, and the length of the microchannels is 0.5m.

[0077]In this embodiment, the specific steps of the preparation method of multivesicular liposomes are as follows:

[0078]1) Preparation of oil phase fluid A: pre-add 80g of soybean phospholipid, 40g of cholesterol, 10g...

Embodiment 2

[0085]The preparation device of the multivesicular liposome in this embodiment is asfigure 1Shown. Among them, the treatment tank A 111 and the treatment tank B121 are 2L reactors, and the treatment tank C 131 is a 15L conventional small test reactor; the number n of the micro-reaction group 210 is 6; among them, the thermostatic tube (A 114, B 124, C 134) has a length of 0.2m and a diameter of 4mm; the diameter of the delay tube 213 is 2mm and a length of 6m; the microchannel cross-sectional equivalent diameter of the microchannel reactor 211 is 3mm, and the microchannel length is 2m; The device 212 has a plurality of microchannels, the cross-sectional equivalent diameter of the microchannels is 4mm, and the length of the microchannels is 0.8m.

[0086]In this embodiment, the specific steps of the preparation method of multivesicular liposomes are as follows:

[0087]1) Preparation of oil phase fluid A: In advance, 40g egg yolk phospholipids, 10g cholesterol, 5g tricaprylin, 1g Tween 80,...

Embodiment 3

[0094]The preparation device of the multivesicular liposome in this embodiment is asfigure 1Shown. Among them, the treatment kettle A 111 and the treatment kettle B121 are 2L reaction kettles, and the treatment kettle C 131 is a 15L conventional small-scale reaction kettle; the number n of the micro-reaction group 210 is 3; among them, the thermostatic tube (A 114, B 124, C 134) has a length of 0.11m and a diameter of 4mm; the delay tube 213 has a diameter of 4mm and a length of 0.7m; the microchannel cross-sectional equivalent diameter of the microchannel reactor 211 is 9mm, and the microchannel length is 1.9m; The micromixer 212 has a plurality of microchannels, the cross-sectional equivalent diameter of the microchannels is 8mm, and the length of the microchannels is 7m.

[0095]In this embodiment, the specific steps of the preparation method of multivesicular liposomes are as follows:

[0096]1) Preparation of oil phase fluid A: In advance, add 50 g of soybean phospholipid, 20 g of ch...

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Abstract

The invention discloses a blank multivesicular liposome and a preparation method and device thereof, and belongs to the field of pesticide adjuvants. The preparation raw materials of the polycystic lipidosome provided by the invention comprise phospholipid substances, sterol substances, neutral lipidosome, fatty acid ester substances and vitamins; the polycystic lipidosome containing vesicles withhigh electronegativity and steric hindrance can be obtained; coagulation of the multivesicular liposome can be effectively inhibited, the drug release time is prolonged, and the drug effect is improved. The invention provides a blank multivesicular liposome preparation method and device. According to the blank multivesicular liposome preparation method and device, a continuous flow micro-channelreactor is used for replacing traditional high-speed shearing equipment; by means of the device, the vesicle stability and particle size uniformity of the multivesicular liposome can be obviously improved, the vesicle size is reduced, the environment-friendly multivesicular liposome can wrap pesticide active matters and is applied to the field of pesticides.

Description

Technical field[0001]The invention belongs to the field of pesticide additives, and more specifically, relates to a blank multivesicular liposome and a preparation method and device thereof.Background technique[0002]Multivesicular liposomes (MVL) are composed of multiple non-concentric aqueous drug solution vesicles, which are separated by a continuous non-concentric lipid bilayer, and have a high encapsulation rate for water-soluble drugs. In particular, multivesicular liposomes release drugs through ruptured vesicles, while intact vesicles remain intact. The non-concentric topological structure of multivesicular liposomes makes it form a "reservoir" at the injection site. With the continuous degradation of the lipid bilayer, the drug encapsulated in it is gradually released, resulting in a good relief. The drug release effect is avoided while avoiding the sudden release effect, and the effect of controlling the drug release time from a few days to several weeks can be obtained by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/34B01J19/00
CPCA01N25/34B01J19/0093B01J2219/00889
Inventor 王磊曹雄飞秦敦忠
Owner YANGZHOU SPED CHEM
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