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Electrostatic complex fluorescent array sensor and its application

A technology of array sensors and complexes, applied in the direction of fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of low sensitivity, high production cost, and poor reproducibility, and achieve high accuracy and sensitivity, low cost, and repeatability high effect

Active Publication Date: 2022-06-28
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The array sensors reported in the literature often require multiple sensing elements, which are cumbersome to operate, high in production costs, narrow in the range of protein detection, low in sensitivity, and poor in reproducibility

Method used

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  • Electrostatic complex fluorescent array sensor and its application
  • Electrostatic complex fluorescent array sensor and its application
  • Electrostatic complex fluorescent array sensor and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Synthesis of fluorescent polymer PPE1

[0041] The fluorescent macromolecular polymer used in the following examples is prepared according to the synthetic route shown in the following figure, by the method of the following steps:

[0042]

[0043] Monomer 1 (1.04 g, 2.35 mmol) and monomer 2 (2.09 g, 2.35 mmol) were dissolved in toluene / DIEA (7.2 mL / 4.9 mL), and Pd (PPh) was added 3 ) 2 Cl 2(4.95 mg, 7.05 μmol) and CuI (2.69 mg, 14.10 μmol) were stirred at room temperature for 24 h. Add saturated NH to the mixture 4 Cl solution and CHCl 3 , the aqueous layer was separated and washed with CHCl 3 extraction. Anhydrous MgSO 4 Dry, filter and remove the solvent under reduced pressure. The crude product was dissolved in a small amount of CHCl 3 , excess n-hexane was slowly added to precipitate, and the precipitation process was repeated three times to obtain compound 3 as a yellow-brown solid (2.63 g, 90%). Molecular weight M n Tested about 1.4x10 4...

Embodiment 2

[0045] Example 2. Synthesis of fluorescent polymer PPE 2

[0046] The fluorescent macromolecular polymer used in the following examples is prepared according to the synthetic route shown in the following figure, by the method of the following steps:

[0047]

[0048] Polymer 4 (25 mg, 0.082 mmol) was dissolved in N,N-dimethylethylenediamine (25 mL) and stirred at 50°C for 24 h. The solvent was removed under reduced pressure, and the crude product was washed with n-hexane. Drying in vacuo gave an orange solid as polymer 5 (52 mg, 0.077 mmol, 94%). 1 H NMR (300 MHz, CDCl3): δ 7.14 (s, 2H), 4.52 (s, 4H), 3.31 (s, 4H), 2.81 (s, 4H), 2.17 (s, 12H). 13 C NMR (300MHz, CDCl3): δ 168.97, 157.57, 118.45, 113.49, 92.94, 90.67, 68.57, 58.10, 44.97, 36.44.

[0049] Polymer 5 (40 mg, 0.1 mmol) was dissolved in dichloromethane (25 mL) and iodomethane (15 mL) was added. Stir overnight at room temperature. The solvent was removed under reduced pressure, washed with hexane, and dried in...

Embodiment 3

[0050] Example 3. Synthesis of fluorescent polymer PPE 3

[0051] The fluorescent macromolecular polymers used in the following examples are prepared according to the synthetic route diagram shown in the attached figure by the following steps:

[0052]

[0053] Monomer 6 (820 mg, 1.00 mmol) and monomer 7 (347 mg, 1.05 mmol) were dissolved in dichloromethane / DIEA / triethylamine (2mL / 2mL / 1mL), and Pd (PPh) was added 3 ) 2 Cl 2 (1.4 mg, 2 μmol) and CuI (0.4 mg, 2 μmol) were stirred at room temperature for 24 h. Polymer 8 (70 mg, 0.082 mmol) was dissolved in N,N-dimethylethylenediamine (25 mL) and stirred at 50°C for 24 h. The solvent was removed under reduced pressure, and the crude product was washed with n-hexane. Drying in vacuo gave an orange solid as polymer 9 (89.4 mg, 0.077 mmol, 94%). Polymer 9 (40 mg, 0.0344 mmol) was dissolved in dichloromethane (25 mL) and iodomethane (15 mL) was added. Stir overnight at room temperature. The solvent was removed under reduced ...

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Abstract

The invention discloses an electrostatic complex fluorescent array sensor and its application. It is composed of water-soluble fluorescent conjugated polymers (PPE) with different side chain substitutions and graphene oxide. It is used for the detection of various proteins and has the characteristics of high accuracy and sensitivity, short detection time, low cost and high repeatability.

Description

technical field [0001] The present invention relates to a fluorescence detection sensor and application, in particular to an electrostatic complex fluorescence array sensor and application. Background technique [0002] Proteins play important roles in living organisms. Detection of proteins is critical for clinical diagnosis, and analysis of specific proteins can provide valuable information for identifying specific physiological and pathological states. On the one hand, too much abnormal protein in the body may lead to certain diseases, such as Alzheimer's disease, which is caused by the accumulation of amyloid in brain tissue. On the other hand, the occurrence and development of certain diseases (such as cancer, prostatitis and hypoalbuminemia, etc.) are often accompanied by the overexpression of various proteins, which can be used as biomarkers for the diagnosis of clinical diseases. Due to the structural diversity of proteins and the complexity of clinical sample syst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L65/00C08K3/04C08G61/02G01N21/64
CPCC08K3/042C08G61/02G01N21/6428C08G2261/18G01N2021/6432C08L65/00
Inventor 韩进松王浩李飞马宗辉黄慧
Owner CHINA PHARM UNIV
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