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Bacterial strain of pathogen of Amomum villosum seedling blight and application thereof

A technology of viridis and pathogenic bacteria, applied in fungi, microorganism-based methods, microorganisms and other directions, can solve the problems of identification of pathogenic bacteria and few studies on biological characteristics, and achieve the goal of improving the survival rate, preventing and controlling the dead phenomenon of seedlings and increasing the yield. Effect

Active Publication Date: 2020-12-29
中国医学科学院药用植物研究所云南分所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the identification and biological characteristics of the pathogenic bacteria of P.

Method used

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  • Bacterial strain of pathogen of Amomum villosum seedling blight and application thereof
  • Bacterial strain of pathogen of Amomum villosum seedling blight and application thereof
  • Bacterial strain of pathogen of Amomum villosum seedling blight and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Separation and purification of Phoam sp.ZO-Laiwu

[0018] After investigation, it was found that seedling blight occurred in all Yangchunsha seedling breeding bases in Xishuangbanna Prefecture, Yunnan Province. Seedling blight started from the lower leaves of Yangchunsha seedlings. In the early stage of the disease, the front of the leaves appeared water-soaked round or irregular yellow Lesions, the diseased spots gradually expand into oblong or strip-shaped dead spots, and in severe cases, the leaves will die until the seedlings die.

[0019] The material of the present invention is collected in Jinghong City, Xishuangbanna Prefecture, Yunnan Province and the four-kilometer vegetable team area of ​​Mengla County to plant the withered and yellow parts of the leaves of the sunflower seedlings. Using the tissue separation method, a 2 mm × 2 mm tissue block at the junction of disease and health was taken, sterilized with 75% alcohol for 30 seconds, sterilized wit...

Embodiment 2

[0020] Example 2 Pathogenicity determination of Phoam sp.ZO-Laiwu

[0021] The pathogenicity of the tested strains was determined according to the microbial pathogenicity determination conditions proposed by Koch and Smith. Place the tested strain in PDA medium for culture, and when the colony grows to half of the petri dish, take a 0.5cm-diameter bacteria cake along the edge of the colony; take a healthy Yangchun sand seedling, prick the leaves with a sterile needle, and inoculate the bacteria cake to the thorn. For the injured part, the PDA medium block was used as a control, and the uninjured leaves and the stabbed leaves were used as a control. Wet absorbent cotton was used to moisturize, and each treatment was repeated 3 times, and the disease was recorded and observed by taking pictures. The diseased leaves were re-separated and purified, and compared with the original pathogenic bacteria.

[0022] The results showed that the pathogenic fungus mycelium block needled th...

Embodiment 3

[0023] Example 3 Identification of Phoam sp.ZO-Laiwu

[0024] 1. Ecological identification of pathogenic bacteria

[0025] The pathogenic bacteria were inoculated on PDA medium and oatmeal agar medium (oatmeal 30g, agar 20g, water 1000ml), cultured in the dark at 25°C for 10 days and 7 days respectively in a constant temperature incubator, observed and photographed. Record the colony shape and color of pathogenic bacteria as the basis for ecological identification of pathogenic bacteria.

[0026] The results showed that when the strain was grown on PDA medium, the fungus cake was white at the beginning, and the hyphae were white flocculent, and then black spots appeared.

[0027] 2. Multi-gene sequence analysis of pathogenic bacteria

[0028] Scrape 0.1 g of pathogenic mycelia on the PDA medium, extract genomic DNA according to the method of the fungal genome Omega kit (Shanghai Yubo Biotechnology Co., Ltd.), and store it at 4°C for later use. ITS5 (5′-GGAAGTAAAAGTCGTAACAAG...

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Abstract

The invention discloses a pathogen of Amomum villosum seedling blight, specifically Phoam sp.ZOLaiwu, which is preserved in China Center for Type Culture Collection (CCTCC), has a preservation numberof CCTCC NO: M2020287 and a preservation date of July 21, 2020, and is taxonomically named as named as Phoma. According to the invention, the pathogen Phoam sp.ZOLaiwu of Amomum villosum seedling blight is beneficial to the prevention and control of seedling withering in Amomum villosum seedling stage, improves the survival rate of the Amomum villosum seedlings, further studies on the prevention and control of Amomum villosum seedling blight, and then improves the yield of Amomum villosum.

Description

technical field [0001] The invention relates to the technical field of bacterial strain screening, and more specifically relates to a bacterial strain of the fungus pathogenic fungus and its application. Background technique [0002] Amomum villosum Lour. is a perennial herb of the Zingiberaceae genus Amomum L, and is one of the four major southern medicines in China. Yangchunsha is a medicinal and edible material. The dried and ripe fruit is used as medicine and food. It tastes pungent and warm, and returns to the spleen, stomach, and kidney meridians. Amomum fruit is oval or oval, with inconspicuous triangular edges connecting three protruding surfaces, dividing Amomum fruit into three parts, 1.5-2 cm long, 1-1.5 cm in diameter, and the surface of the fruit is brown , with soft spine-like protrusions, perianth remnants at the top, and fruit stalks at the base; the peel is soft and thin, with a special aroma; the seeds are wrapped into clusters, with three obtuse edges, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12R2001/645C12N1/145
Inventor 张丽霞李戈王延谦王艳芳李荣英李爽杨春勇彭建明严珍
Owner 中国医学科学院药用植物研究所云南分所
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