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A kind of Bacillus amyloliquefaciens bacterial strain with antibacterial activity and application thereof

A technology for dissolving amyloid spores and strains, applied in the field of microorganisms, can solve the problems of limited application range of antagonistic action, low yield of antibacterial substance lipopeptide, unfavorable development and the like, and achieves the effect of good application prospect.

Active Publication Date: 2022-05-17
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For the reported Bacillus, especially Bacillus amyloliquefaciens strains, on the one hand, its antagonism against common pathogenic bacteria in the growth and development of food crops and economic crops has a limited scope of application; on the other hand, the antibacterial substance lipopeptide it produces The yield is low and the cost of separation is high, which is not conducive to the development of live bacterial preparations or preparations mainly composed of active substances produced by bacteria

Method used

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  • A kind of Bacillus amyloliquefaciens bacterial strain with antibacterial activity and application thereof
  • A kind of Bacillus amyloliquefaciens bacterial strain with antibacterial activity and application thereof
  • A kind of Bacillus amyloliquefaciens bacterial strain with antibacterial activity and application thereof

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Embodiment Construction

[0023] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. The examples are intended to enable those skilled in the art to better understand the technical solutions of the present invention, rather than to limit the protection scope of the present invention.

[0024] (1) Isolation and identification of strains

[0025] 1. Sample collection and pretreatment

[0026] Take 1g of fresh Suaeda salsa leaf tissue collected from the saline-alkali land in Yulin City, Shaanxi Province, first surface-sterilize with 75% ethanol for 3 minutes, then surface-sterilize with 5% sodium hypochlorite for 5 minutes, and finally rinse with sterile water three times. Thoroughly grind the surface-sterilized leaves with 3 mL of sterile water in a mortar, centrifuge the resulting grinding liquid at 8000 r / min for 5 min, and take the supernatant for later use.

[0027] 2. The isolation of Bacillus amyloliquefaciens wxy-b3, the ...

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Abstract

The invention discloses a Bacillus amyloliquefaciens strain with antibacterial activity and its application. In the present invention, the antibacterial activity of Bacillus amyloliquefaciens wxy-b3 (the preservation number is CGMCC No. 20041) isolated from the leaf tissue of Sua Rot mold, Escherichia coli and Bacillus cereus have inhibitory effects, and the yield of antibacterial lipopeptides produced by fermentation is high, which can be used to prepare antibacterial agents to prevent crop yield reduction due to diseases.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to the isolation, identification and application of a strain of Bacillus amyloliquefaciens with broad-spectrum antibacterial activity. Background technique [0002] As an integral part of the microbial ecology in plants, endophytes play an important role in the stability of plants. They generally occupy a favorable ecological niche and widely exist in plants. Most endophytes can secrete metabolites with different functions. , so as to promote plant growth, antagonize plant pathogenic microorganisms, insects and nematodes, and jointly repair the environment with plants to improve plant stress resistance. Endophytic Bacillus is a type of plant endophytic bacteria that has been studied more, and plays an important role in crop biological control, natural drug development, and environmental restoration. [0003] As it becomes more and more difficult to isolate new species or strains with ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/02C07K7/06A01N63/22A01N37/46A01P1/00A01P3/00C12R1/07
CPCC12N1/20C12P21/02C07K7/06A01N63/22A01N37/46C12R2001/07C12N1/205Y02A50/30
Inventor 龚国利刘荣杰王旭阳田露
Owner SHAANXI UNIV OF SCI & TECH