Gene marker for acute severe viral myocarditis
A viral myocarditis, acute technology, applied in the field of cardiac medicine, can solve the problems of lack of effectiveness and misdiagnosis in the diagnosis of viral myocarditis, and achieve excellent sensitivity and specificity, and the effect of precise treatment
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Embodiment 1
[0023] The groups were divided into: healthy control group (peripheral blood of normal people), acute severe viral myocarditis group (peripheral blood of patients with acute severe viral myocarditis) and treatment recovery group (peripheral blood of patients whose symptoms improved significantly after treatment).
[0024] 1. Extracting peripheral blood mononuclear cells from blood
[0025] (1) Add 4ml of lymphocyte separation solution into the centrifuge tube, slowly add 4ml of blood sample into the separation solution along the wall of the centrifuge tube;
[0026] (2) Place the centrifuge tube in a centrifuge and centrifuge at 2000rpm for 30min;
[0027] (3) Carefully collect milky white lymphocyte layer cells, put them into a new 2ml centrifuge tube, add PBS to wash, centrifuge at 1500rpm for 10min, repeat twice to obtain peripheral blood mononuclear cells;
[0028] 2. RNA extraction
[0029] (1) Add 1ml TRIZOL reagent, pipette repeatedly until the liquid is clear, and le...
Embodiment 2
[0060] Detection of the expression difference of LINC02092 between healthy control group and acute myocardial infarction group
[0061] The steps of mononuclear cell extraction, RNA extraction, reverse transcription reaction, and fluorescent quantitative PCR reaction are the same as in Example 1.
[0062] Experimental results such as Figure 4 As shown, it can be seen from the figure that there is no significant difference in the expression of LINC02092 in the mononuclear cells of the samples of the healthy control group and the samples of the acute myocardial infarction group (the relative expression of LINC02092 in the acute myocardial infarction group is 1.092±0.065, P =0.3542).
[0063] In summary, LINC02092 can be used in the diagnosis and prognosis of acute severe viral myocarditis, and can be used to distinguish patients with acute severe viral myocarditis from patients with acute myocardial infarction.
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