Gene marker for acute severe viral myocarditis

A viral myocarditis, acute technology, applied in the field of cardiac medicine, can solve the problems of lack of effectiveness and misdiagnosis in the diagnosis of viral myocarditis, and achieve excellent sensitivity and specificity, and the effect of precise treatment

Active Publication Date: 2021-01-05
山东大学齐鲁医院(青岛)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most infected patients only have mild clinical symptoms, and only a small number of more severe infected patients will have obvious complications, so there is currently a lack of effective and specific detection methods for the diagnosis of viral myocarditis
Moreover, severe viral myocarditis is easily misdiagnosed with acute myocardial infarction.

Method used

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  • Gene marker for acute severe viral myocarditis
  • Gene marker for acute severe viral myocarditis
  • Gene marker for acute severe viral myocarditis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The groups were divided into: healthy control group (peripheral blood of normal people), acute severe viral myocarditis group (peripheral blood of patients with acute severe viral myocarditis) and treatment recovery group (peripheral blood of patients whose symptoms improved significantly after treatment).

[0024] 1. Extracting peripheral blood mononuclear cells from blood

[0025] (1) Add 4ml of lymphocyte separation solution into the centrifuge tube, slowly add 4ml of blood sample into the separation solution along the wall of the centrifuge tube;

[0026] (2) Place the centrifuge tube in a centrifuge and centrifuge at 2000rpm for 30min;

[0027] (3) Carefully collect milky white lymphocyte layer cells, put them into a new 2ml centrifuge tube, add PBS to wash, centrifuge at 1500rpm for 10min, repeat twice to obtain peripheral blood mononuclear cells;

[0028] 2. RNA extraction

[0029] (1) Add 1ml TRIZOL reagent, pipette repeatedly until the liquid is clear, and le...

Embodiment 2

[0060] Detection of the expression difference of LINC02092 between healthy control group and acute myocardial infarction group

[0061] The steps of mononuclear cell extraction, RNA extraction, reverse transcription reaction, and fluorescent quantitative PCR reaction are the same as in Example 1.

[0062] Experimental results such as Figure 4 As shown, it can be seen from the figure that there is no significant difference in the expression of LINC02092 in the mononuclear cells of the samples of the healthy control group and the samples of the acute myocardial infarction group (the relative expression of LINC02092 in the acute myocardial infarction group is 1.092±0.065, P =0.3542).

[0063] In summary, LINC02092 can be used in the diagnosis and prognosis of acute severe viral myocarditis, and can be used to distinguish patients with acute severe viral myocarditis from patients with acute myocardial infarction.

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Abstract

The invention provides a gene marker for acute severe viral myocarditis, and belongs to the technical field of cardiac medicines. By detecting peripheral blood mononuclear cells of patients with acutesevere viral myocarditis, peripheral blood mononuclear cells of normal person and peripheral blood mononuclear cells of patients with acute severe viral myocarditis, but significant improvement aftertreatment, the invention demonstrates that LINC02092 gene has obvious difference and excellent diagnostic value in term of diagnosis and prognosis of acute severe viral myocarditis patients, so thatthe LINC02092 gene can be used for preparing diagnostic reagents for diagnosing acute severe viral myocarditis.

Description

technical field [0001] The invention belongs to the technical field of heart medicine, and in particular relates to a gene marker of acute severe viral myocarditis. Background technique [0002] Viral myocarditis is the most common cause of secondary dilated cardiomyopathy, which is a diffuse or focal inflammatory lesion of the myocardium caused by viral infection. In recent years, the incidence of viral myocarditis has increased significantly. A small number of cases have sudden onset and rapid development, and patients will develop severe heart failure and neogenic shock within a short period of time. Moreover, viral myocarditis is a common cardiovascular infectious disease in forensic pathological identification work, and it is one of the main causes of sudden death in adolescents. Since most infected patients only have mild clinical symptoms, and only a small number of more severe infected patients will have obvious complications, so there is currently no effective and...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 王颖翠
Owner 山东大学齐鲁医院(青岛)
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