Novel coronavirus detection method and system based on luminous quantum dot material

Abstract

The invention discloses a novel coronavirus detection method and system based on a luminous quantum dot material, and the method comprises the following steps: adding CdTe quantum dots into a PBS buffer solution, and uniformly mixing to obtain a first mixed solution; adding a specific antibody of the spike protein into the first mixed solution, uniformly mixing to obtain a second mixed solution, and testing the PL spectral intensity of the second mixed solution; dropwise adding a sample to be detected into the second mixed solution, uniformly mixing to obtain a third mixed solution, and testing the PL spectral intensity of the third mixed solution; and if the PL spectral intensity of the third mixed solution is less than the PL spectral intensity of the second mixed solution, judging thatthe sample to be detected contains the novel coronavirus. According to the detection method provided by the invention, the response time is less than 2 minutes, and the response to the to-be-detectedsample can be quickly realized; the requirement on a detection instrument is low; and the detection limit on the novel coronavirus reaches 1 * 10 < 16 > mg / ml.

Description

technical field [0001] The invention relates to the field of virus detection, in particular to a novel coronavirus detection method and system based on luminescent quantum dot materials. Background technique [0002] Rapid and accurate identification or identification of potential pathogens is critical for disease control, patient treatment, and prevention of the epidemic of emerging infectious diseases such as severe polar respiratory syndrome coronavirus, SARS-COV. [0003] Currently, clinical virus detection technologies mainly include reverse transcription polymerase chain reaction (RT-PCR), POC technology based on antibody detection and chest computed tomography (CT). However, although these technical means can achieve relatively accurate detection of viruses, they have high requirements for professional instruments and equipment. At the same time, the detection time of these technical means is long, making it difficult to achieve rapid detection of the samples to be te...

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a novel coronavirus detection method and system based on luminescent quantum dot materials, aiming at solving the problem of long detection time and high cost of novel coronavirus in the prior art. The problem of poor detection sensitivity

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