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Application of phenolic compounds as nematicides

A technology for phenolic compounds and nematicides, which is applied in the application field of phenolic compounds as nematicides, can solve serious problems and the like, and achieves the effect of improving activity and facilitating popularization and application.

Active Publication Date: 2021-01-19
沈阳恩柽研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of low-toxicity and environmental protection of original medicines and dosage forms, the use of highly toxic and highly toxic original medicines such as carbamate pesticides and organophosphorus pesticides has been gradually restricted or banned. At the same time, many dosage forms such as fumigants It is difficult to be effective against nematodes in an open air and non-closed environment, and it is facing the dilemma of withdrawing from the stage; thus, nematodes on many crops such as soybeans, corn, cotton, fruits and vegetables will become more and more serious, and forestry quarantine pine wood nematodes are also urgently needed. Need for new nematicidal active substances
At present, pinaceae plants in pine wood nematode-infested areas can only be harvested for treatment. The diseased trees that are harvested for treatment must be crushed or burned on the spot in the mountain field. The burned trees must be photographed and archived throughout the process. The wood can only be used in the local area, it is forbidden to use it across provincial administrative regions, and it is forbidden to use the diseased wood to process boards in mountain farms

Method used

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  • Application of phenolic compounds as nematicides
  • Application of phenolic compounds as nematicides
  • Application of phenolic compounds as nematicides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, Toxic activity and LC50 determination of 2,6-di-tert-butyl-p-cresol to pine xylophilus

[0035] Pharmacy preparation:

[0036] The preparation of the following experimental group of medicaments is to prepare 2,6-di-tert-butyl-p-cresol aqueous solutions with different concentrations through an aqueous solution containing DMSO and Tween 80; wherein, the aqueous solution The content of DMSO in the medium is 20%, and the content of Tween 80 is 10%.

[0037] test group:

[0038] 1) 100 mL of 2,6-di-tert-butyl-p-cresol aqueous solution with a concentration of 10 g / L;

[0039] 2) 100 mL of 2,6-di-tert-butyl-p-cresol aqueous solution with a concentration of 20 g / L;

[0040] 3) 100 mL of 2,6-di-tert-butyl-p-cresol aqueous solution with a concentration of 30 g / L.

[0041] Control group: use the corresponding system solvent of each experimental group as the blank control of each experimental group.

[0042] Preparation of the nematode suspension: Suspend the pine woo...

Embodiment 2

[0047] Embodiment 2, phenol (phenol) to the mensuration of the poisonous activity of pine xylophilus and LC50

[0048] Drug preparation: phenol was prepared into 9 concentrations of 0, 0.1, 1, 2, 3, 4, 5, 15 and 30 g / L with sterilized water, of which 0 g / L was used as the control.

[0049] Preparation of the nematode suspension: Suspend the pine wood nematode on the surface of a 9cm PDA culture dish with an appropriate amount of sterilized water, and then separate the live pine wood nematode suspension through the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes under the microscope, and finally quantify the nematode suspension to 1000 nematodes / mL with sterilized water. The nematodes are in the J3 stage, and a few are in the J2 and J4 stages, and they are ready for use at ...

Embodiment 3

[0054] Embodiment 3, sodium phenate poisonous activity and LC50 determination to pine xylophilus

[0055] Drug preparation: Sodium phenate was prepared into 5 concentrations of 0, 1, 2, 3 and 4 g / L with sterilized water, and 0 g / L was used as the control.

[0056] Preparation of the nematode suspension: Suspend the pine wood nematode on the surface of a 9cm PDA culture dish with an appropriate amount of sterilized water, and then separate the live pine wood nematode suspension through the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes under the microscope, and finally quantify the nematode suspension to 1000 nematodes / mL with sterilized water. The nematodes are in the J3 stage, and a few are in the J2 and J4 stages, and they are ready for use at 4°C.

[0057] Drug treatm...

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PUM

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Abstract

The invention belongs to the field of pesticides, and particularly relates to an application of phenol compounds as nematicides. The invention further discloses the application of one or more of the phenolic compounds represented by the general formula I or salts thereof in serving as the nematicides. The general formula I is shown in the specification. The compounds are suitable for being used asthe nematicides, especially sodium phenolate with good activity is commonly used as a preservative, and 3-hydroxybenzoic acid and 4-hydroxybenzoic acid are antioxidants which are widely applied at present, and the compounds are low in toxicity, low in price and easy to obtain.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to the application of a phenolic compound as a nematode (especially pine wood nematode (Bursaphelenchus xylophilus)) agent. Background technique [0002] Nematodes belong to the phylum Nematodes in terms of taxonomic status. There are many kinds of nematodes and their geographical distribution is extremely wide. Plant nematode diseases have now developed into an important factor restricting the healthy and sustainable development of global agriculture and forestry. The annual economic loss is conservatively estimated to be about 157 billion Big dollar. Meloidigyne incognita, M. javanica, M. arenaria and M. hapla are the most common and dominant species in my country. Nematodes are small in size and the harmful part is often located at the roots of plants submerged in the soil. Early damage is not easy to detect. Although its own mobility is very limited (its own annual moving ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/08A01N37/40A01P5/00
CPCA01N31/08A01N37/40
Inventor 李世友赵福强李帅强王萍韩璐
Owner 沈阳恩柽研究院有限公司
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