Method for culturing microalgae based on artificial intelligence microalgae mixing system
An artificial intelligence, hybrid system technology, applied in the field of artificial intelligence-based microalgae cultivation, can solve problems such as waste of energy consumption
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Embodiment 1
[0066]Example 1 The influence of temperature and light intensity on the growth of spirulina
[0067]First prepare the following medium: 25.2g L-1NaHCO3, 2.5g L-1NaNO3, 0.04g L-1CaCl2·2H2O,1.0g L-1K2SO4,0.5g L-1K2HPO4,0.2g L-1MgSO4·7H2O,0.01g L-1FeSO4·7H2O,1.979g L-1MoCl2·4H2O, 3.092g L-1H3BO3, 0.484g L-1NaMo4·2H2O, 0.23g L-1ZnSO4·7H2O, 0.183g L-1NaVO3, 0.048g L-1CoCl2·6H2O, 0.2g L-1CuSO4·5H2O.
[0068]The medium was injected into a rectangular tube photobioreactor with a height of 10 cm, a width of 5.0 cm, and a length of 20 cm. The mixing of the reaction was provided by a rocker-type shaker with a rotating speed of 60 rpm and a continuous working mode. Then insert fresh Spirulina FACHB-314 into the reactor, and control the final inoculation volume and inoculation density to 200ml and 0.1g L respectively-1. Finally, at 50, 200 and 400μmol·m-2·S-1Under three continuous light intensities, to investigate the influence of temperature of 15, 20, 25, 30, 35, 40 ℃ on the growth of spirulina, the...
Embodiment 2
[0070]Example 2 Cultivation of Spirulina in Smart Open Pool
[0071]Inject 400L of the medium described in Example 1 into the cultivation area of 2.0m2In the racetrack pool, the thickness of the liquid layer after the medium is injected is 20.0cm. The mixing of the culture solution in the open pond is driven by its stirring paddle, which is connected to the controller of the intelligent mixing system through a data line and operates under instructions from the controller. In addition, the controller is also connected to a light intensity meter and a thermometer, respectively, for the detection and acquisition of light intensity and temperature, where the measured temperature is the temperature of the culture solution. The controller will control the rotation speed of the stirring paddle based on the real-time detected data and through a pre-programmed program. The control is based on the experimental results derived from Example 1. The specific intelligent open pool mixing control st...
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