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Application of carnosine to protecting activity of plant exosomes

A technology of exosomes and plants, applied in the field of biomedicine, can solve the problems of difficult to ensure activity, limit the application of plant exosomes and the efficacy of products, and achieve good anti-aging effects

Pending Publication Date: 2021-01-22
西安博和医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to guarantee the activity of plant exosomes at room temperature, which limits the application of plant exosomes and the efficacy of products. Therefore, there are few researches and applications on plant exosomes at present.

Method used

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  • Application of carnosine to protecting activity of plant exosomes
  • Application of carnosine to protecting activity of plant exosomes
  • Application of carnosine to protecting activity of plant exosomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment provides a composition for anti-aging, according to parts by weight, the composition comprises:

[0039] 0.1 parts of grape exosomes, 0.1 parts of carnosine, 0.5 parts of glycerin, 0.5 parts of butanediol, 0.1 parts of phenoxyethanol, 93 parts of water;

[0040] The preparation method of the grape exosomes is as follows:

[0041] Weigh 100g of fresh grapes and pour them into a crusher, add 100mL sterilized PBS to it, and after crushing and homogenizing, centrifuge at 3000×g, 4°C for 10min to remove larger residues; , centrifuged at 4°C for 40 min; the supernatant was ultracentrifuged at 120,000×g, 4°C for 60 min, then resuspended in 1 mL of sterile PBS and then ultracentrifuged at 120,000×g, 4°C for 60 min; after centrifugation, washed with 1 mL of sterile PBS Resuspend the pellet to obtain grape exosomes.

Embodiment 2

[0043] This embodiment provides a composition for anti-aging, according to parts by weight, the composition comprises:

[0044]0.5 parts of grape exosomes, 0.5 parts of carnosine, 3 parts of glycerin, 3 parts of butanediol, 0.4 parts of phenoxyethanol, 96 parts of water;

[0045] The preparation method of the grape exosomes is as follows:

[0046] Weigh 100g of fresh grapes and pour them into a crusher, add 100mL sterilized PBS to it, and after crushing and homogenizing, centrifuge at 3000×g, 4°C for 10min to remove larger residues; , centrifuged at 4°C for 40 min; the supernatant was ultracentrifuged at 120,000×g, 4°C for 60 min, then resuspended in 1 mL of sterile PBS and then ultracentrifuged at 120,000×g, 4°C for 60 min; after centrifugation, washed with 1 mL of sterile PBS Resuspend the pellet to obtain grape exosomes.

Embodiment 3

[0048] This embodiment provides a composition for anti-aging, according to parts by weight, the composition comprises:

[0049] 0.2 parts of grape exosomes, 0.3 parts of carnosine, 2 parts of glycerin, 2 parts of butanediol, 0.3 parts of phenoxyethanol, 95 parts of water;

[0050] The preparation method of the grape exosomes is as follows:

[0051] Weigh 100g of fresh grapes and pour them into a crusher, add 100mL sterilized PBS to it, and after crushing and homogenizing, centrifuge at 3000×g, 4°C for 10min to remove larger residues; , centrifuged at 4°C for 40 min; the supernatant was ultracentrifuged at 120,000×g, 4°C for 60 min, then resuspended in 1 mL of sterile PBS and then ultracentrifuged at 120,000×g, 4°C for 60 min; after centrifugation, washed with 1 mL of sterile PBS Resuspend the pellet to obtain grape exosomes.

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Abstract

The invention provides an application of carnosine to protecting the activity of plant exosomes. The invention also provides a composition for resisting senility, and the composition comprises the carnosine and the plant exosomes. The composition for resisting senility provided by the invention has a very good senility resisting effect.

Description

technical field [0001] The invention relates to the field of biomedicine. Specifically, the present invention relates to the use of carnosine in protecting plant exosome activity. Background technique [0002] Skin aging can be divided into endogenous aging caused by internal factors and exogenous aging secondary to external factors. Skin aging is usually reflected in the reduction of skin elasticity and the generation of skin wrinkles. The reduction of collagen and elastin will Causes the reduction of skin elasticity and the generation of skin wrinkles. Collagen and elastin are regulated by collagenase and elastase. Collagenase and elastase can cause the degradation of collagen and elastin, resulting in the reduction of collagen and elastin, resulting in wrinkles. Skin Reduced elasticity. [0003] Exosomes are goblet-shaped vesicles secreted by a variety of living cells with a diameter of about 30-100 nm and a double-layer membrane structure. They have a lipid bilayer str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/64A61Q19/08
CPCA61K8/9789A61K8/64A61Q19/08A61K2800/592
Inventor 张婷婷吴田田
Owner 西安博和医疗科技有限公司
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