Biological particle purification device and purification method

A technology of biological particles and purification devices, applied in the field of biochemical analysis, can solve the problems of easy contamination, limited magnetic field action distance, low degree of automation, etc., and achieve the effect of easy derivation

Pending Publication Date: 2021-01-22
GUANGZHOU GUANGLI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is to adopt the test tube method, which needs to be done manually, which is not suitable for large-scale production and operation
Second, the working distance of the magnetic field is limited. If the volume of the cell sample is very large, it is not suitable to use this method to collect cells.
Third, the non-specific binding (background binding) of magnetic beads is often high, and the purity of isolated cells is affected
Fourth, this method is suitable for batch operations, with a low degree of automation and easy contamination
At present, there are cell separation methods with different principles on the market, as well as the traditional centrifuge collection method. The two are operated separately, and human intervention must be performed during the operation, and the workload of manual operation is large.

Method used

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  • Biological particle purification device and purification method
  • Biological particle purification device and purification method
  • Biological particle purification device and purification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Such as figure 1 , 2 As shown, the biological particle purification device includes at least one set of affinity chromatography columns 100 and a centrifugal system. The biological particle purification device includes: a purification system and a centrifugal system. Affinity chromatography column 100 is provided with packing, and packing is used for adsorbing biological particles, and packing has chromatography channel 300, and affinity chromatography column 100 is provided with liquid inlet 110 and liquid outlet 120, and liquid inlet 110 is connected with chromatography The channel 300 communicates, the liquid outlet 120 communicates with the chromatography channel 300 , the centrifuge system includes a centrifuge and other auxiliary equipment, and the centrifuge 400 is connected to the liquid outlet 120 . The stock solution 700 to be purified enters from the liquid inlet 110 and flows through the chromatographic channel 300 for purification.

[0066] In this embod...

Embodiment 2

[0088] The difference between embodiment two and embodiment one is:

[0089] Such as Figure 3-6 As shown, the filler is a membrane carrier 210, and the affinity chromatography column 100 is provided with a membrane carrier 210 to replace the microparticle carrier 200 (the technical characteristics of the microparticle carrier 200 described in Example 1 can be replaced on the membrane carrier 210 for use, in this embodiment The example will not be described in detail.), the membrane carrier 210 is curled into a loose scroll and placed in the affinity chromatography column 100, and the gap formed by the membrane carrier 210 forms the chromatography channel 300, such as Figure 4 , 5 As shown, the membrane material can be fixed into a roll shape by using commonly used brackets, buckles, welding, bonding or other methods. The affinity chromatography column 100 is provided with a liquid inlet 110 and a liquid outlet 120, the liquid inlet 110 is communicated with the chromatograp...

Embodiment 3

[0091] The difference between embodiment three and embodiment two is:

[0092] Such as Figure 7 As shown, the filler is a membrane carrier 210, and the membrane carrier 210 is provided with cavities 213, and these cavities 213 form a chromatography channel 300, and the stock solution 700 to be purified enters from the center of the reel, flows out after passing through the hollow chromatography channel 300, and the membrane carrier 210 is provided with specific antibodies, and the target biological particles in the stock solution 700 to be purified bind to the specific antibodies attached to the membrane carrier 210 . In this embodiment, the membrane carrier 210 includes a laminated lining film 211 and a microporous membrane 212, the lining membrane 211 is mesh-shaped, and the lining membrane 211 is used to provide support for the microporous membrane 212, and the stock solution 700 to be purified can easily pass through the lining membrane 211 mesh. The microporous membran...

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Abstract

The invention relates to a biological particle purification device and a purification method. The biological particle purification device comprises the following parts: a purification system, and a centrifugation system. The purification system consists of at least one group of affinity columns, wherein the affinity columns are filled with fillers used for adsorbing biological particles and have chromatography channels; the affinity columns are provided with liquid inlets and liquid outlets; the liquid inlets are communicated with the chromatography channels; and the liquid outlets are communicated with the chromatography channels. The centrifugal system is in butt joint with the liquid outlets. The biological particle purification device forms a closed automatic process, so that pollutioncan be avoided; and moreover, continuous automatic purification operation can be achieved so as to ensure high production efficiency of the purification method.

Description

technical field [0001] The invention belongs to the field of biochemical analysis, and in particular relates to a purification device and a purification method of biological particles. Background technique [0002] At present, with the advancement of medicine, we can use some biological particles to treat certain diseases or conduct certain researches, including but not limited to cells, antibodies, antigens, viruses, prions, etc. Among them, cell therapy has shown great application value in the treatment of cancer, neurological diseases, tissue damage, diabetes and other diseases. Immune cells (T cells) and stem cells are currently the two most commonly used types of cells for therapeutic purposes. Generally speaking, the cells used for treatment have to undergo separation and purification, in vitro expansion and other processing methods before being infused back into the patient. In this process, isolation and purification of cells (or other biological particles) is ofte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/0783B04B5/00
CPCB04B5/00C12M23/58C12M47/04C12N5/0636C12N5/0646C12N2509/00C12N2509/10
Inventor 宗益农周征宇
Owner GUANGZHOU GUANGLI BIOTECH
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