A cell separation device
A technology for separating devices and cells, applied in the field of cell culture, can solve the problems of species limitation, cumbersome cell transfer process, long-time cell adsorption and elution process, etc., to achieve the effects of improving efficiency, easy viewing, and improving recovery rate.
Active Publication Date: 2022-05-10
北京龙迈达斯科技开发有限公司
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Problems solved by technology
[0002] In the prior art, it is often necessary to separate cells for follow-up experiments; at present, physical methods such as density gradient centrifugation, flow cytometry, etc. are often used in laboratories, but with the above methods, the proportion of cell separation generally does not exceed 80%. There are also types of restrictions
There is a technology for cell separation using antigen-antibody reaction, but the transfer process of target cells is cumbersome, and the process of cell adsorption and elution takes a long time
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[0065] Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, the same numerals in different drawings refer to the same or similar elements unless otherwise indicated. The implementations described in the following exemplary examples do not represent all implementations consistent with the present invention. Rather, they are merely examples of apparatuses and methods consistent with aspects of the invention as recited in the appended claims.
[0066] The terminology used in the present invention is for the purpose of describing particular embodiments only and is not intended to limit the invention. As used herein and in the appended claims, the singular forms "a", "the", and "the" are intended to include the plural forms as well, unless the context clearly dictates otherwise. It should also be understood that the term "and / or" as use...
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The invention provides a cell separation method, comprising the following steps: antibody fixation; device preparation; cell adsorption; and cell elution. The present invention also provides a cell separation device, which includes a housing and an adsorption assembly. A cell pool and an elution pool are arranged in the housing. A pool wall is formed between the cell pool and the elution pool. A gap is provided on the pool wall. The pool at the gap is The wall is provided with a rotating shaft, and the adsorption assembly includes an adsorption device, which is located at the notch and matches with the notch, and the adsorption device is connected with the rotation shaft on the pool wall, so that it can enter the cell pool and the elution pool For the exchange of part of the structure, the adsorption device is provided with a load tank, which is used to load the antibody. The cell separation method and device can simultaneously adsorb and elute cells, saving time and improving efficiency; the eluted adsorption device is moved into the cell pool again to re-adsorb cells, and the method can perform cyclic adsorption and elution, improving Cell Separation Ratio.
Description
technical field [0001] The invention relates to the field of cell culture, in particular to a cell separation device. Background technique [0002] In the prior art, it is often necessary to separate cells for follow-up experiments; at present, physical methods such as density gradient centrifugation, flow cytometry, etc. are often used in laboratories, but with the above methods, the proportion of cell separation generally does not exceed 80%. There are also types of restrictions. There have been techniques for cell separation using antigen-antibody reactions, but the transfer process of target cells is cumbersome, and the process of cell adsorption and elution takes a long time. [0003] Therefore, there is an urgent need in the art for a cell separation device. [0004] In view of this, the present invention is proposed. Contents of the invention [0005] The object of the present invention is to provide a cell separation device to solve at least one of the above tec...
Claims
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IPC IPC(8): C12N5/00C12M1/10C12M1/00
CPCC12N5/00C12M23/38C12M23/50C12M33/00C12M47/04C12N2509/00
Inventor 毛静涛
Owner 北京龙迈达斯科技开发有限公司