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Method for preparing N<epsilon>-lysine-based surfactant by using microreactor

A surfactant and lysine-based technology, applied in the field of enzyme-catalyzed amidation of lysine, can solve the problems of low selectivity, long reaction time, low yield, etc., achieve simple preparation process and improve selectivity , Optimizing the effect of the process

Active Publication Date: 2021-01-22
NANJING ADVANCED BIOLOGICAL MATERIALS & PROCESS EQUIP INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method to obtain N ε -The method of fatty acyl lysine, thereby overcomes the technical problems such as too long reaction time, low yield, low selectivity existing in the amidation lysine process at present

Method used

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  • Method for preparing N&lt;epsilon&gt;-lysine-based surfactant by using microreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Fill 0.495 g of immobilized enzyme Novozyme 435 with a particle size of 0.3 to 0.9 mm into an immobilized enzyme microreactor with an inner diameter of 3.8 mm and a length of 150 mm (retention volume is 1.2 mL), connect the device, and use n-hexane solvent Flush the pipes. The concentration is 80mmol·L -1 The lysine is dissolved in water to obtain the mixed solution A, which is transferred to the device 3, and the concentration is 20mmol·L -1 octanoic acid dissolved in n-hexane to obtain mixed solution B Move it into device 4, adjust the flow rate of A and B to be 0.2ml / min, and start the reaction at a reaction temperature of 40°C in the reactor. After 10 minutes of stable reaction, collect the reaction solution, take the upper layer, and leave it in the refrigerator to precipitate unreacted octanoic acid, filtered, and the filtrate was rotary evaporated, vacuum-dried, and finally white N ε - Octanoyllysine solids, 91% conversion.

Embodiment 2

[0027] Fill 0.495 g of immobilized enzyme Novozyme 435 with a particle size of 0.3 to 0.9 mm into an immobilized enzyme microreactor with an inner diameter of 3.8 mm and a length of 150 mm (retention volume is 1.2 mL), connect the device, and use n-hexane solvent Flush the pipes. The concentration is 80mmol·L -1 The lysine is dissolved in water to obtain the mixed solution A, which is transferred to the device 3, and the concentration is 20mmol·L -1 of capric acid solution Dissolve in n-hexane to obtain mixed solution B and transfer it to device 4, adjust the flow rate of A and B to be 0.2ml / min, start the reaction at the reaction temperature in the reactor at 40°C, collect the reaction solution after the reaction is stable for 10 minutes, and take the upper layer , standing in the refrigerator, unreacted capric acid was separated out, filtered, the filtrate was rotary evaporated, vacuum dried, and finally white N ε - Decanoyl lysine solid, 92% conversion.

Embodiment 3

[0029] Fill 0.495 g of immobilized enzyme Novozyme 435 with a particle size of 0.3 to 0.9 mm into an immobilized enzyme microreactor with an inner diameter of 3.8 mm and a length of 150 mm (retention volume is 1.2 mL), connect the device, and use n-hexane solvent Flush the pipes. The concentration is 80mmol·L -1 The lysine is dissolved in water to obtain the mixed solution A, which is transferred to the device 3, and the concentration is 20mmol·L -1 The lauric acid was dissolved in n-hexane to obtain the mixed solution B, which was transferred to device 4. The flow rate of A and B was adjusted to 0.2ml / min, and the reaction temperature in the reactor was 40°C to start the reaction. After the reaction was stable for 10 minutes, the reaction solution was collected. Take the supernatant, leave it standing in the refrigerator, separate out unreacted lauric acid, filter, spin the filtrate, and dry it in vacuum to get white N ε - Lauroyl lysine solid, 90% conversion.

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Abstract

The invention provides a method for preparing an N<epsilon>-lysine-based surfactant by using a micro-channel reaction device. The method comprises the following steps that step 1, lysine is dissolvedin water to obtain a mixed solution A; fatty acid is dissolved in an organic solvent to obtain a mixed solution B; lipase is filled into a reaction tube of an immobilized enzyme micro-reactor of the micro-channel reaction device; step 2, the solution A and the solution B obtained in the step 1 are simultaneously pumped into a mixer of the micro-channel reaction device for mixing, and then the mixture enters the immobilized enzyme micro-reactor for an enzyme catalyzed lysine amidation reaction; and step 3, after the reaction is finished, effluent of the reactor is collected, supernatant is taken, and separation and purification are carried out to obtain the N<epsilon>-lysine-based surfactant. According to the method, the micro-reactor is used for carrying out an enzyme catalysis process, sothat a good reaction effect on various fatty acids is achieved, and the high-yield N<epsilon> fatty acyl lysine surfactant can be obtained; and the reaction time is shortened, the preparation processis simple, the selectivity is improved, the process flow is optimized, and the method has the advantages of safety, high efficiency and greenness.

Description

technical field [0001] The invention relates to an enzyme-catalyzed amidation reaction of lysine, in particular to a method for preparing a lysine-based surfactant using a microchannel reaction device. Background technique [0002] Surfactant is one of the largest and most representative chemical products used in the world. Molecules with amphiphilic groups in the general structure can be called surfactants; hydrophilic groups are often polar groups, such as carboxylic acid, sulfonic acid, sulfuric acid, amino or amine groups and their salts, hydroxyl, amide groups, ether bonds, etc. can also be used as polar hydrophilic groups; while hydrophobic groups are often non-polar hydrocarbon chains, aromatic hydrocarbons and other groups with weak hydrophilicity, and when the carbon number of the hydrophobic chain is 8 or more , the hydrophobic performance is better. Surfactants are divided into ionic surfactants (including cationic surfactants and anionic surfactants), nonionic ...

Claims

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Application Information

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IPC IPC(8): C12P13/08
CPCC12P13/08C12P7/6436
Inventor 季栋李玉光郭凯张锴石婷婷沈磊
Owner NANJING ADVANCED BIOLOGICAL MATERIALS & PROCESS EQUIP INST CO LTD
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