RPA (recombinase polymerase amplification) primer and kit for quickly detecting citrus huanglongbing Asian species as well as detection method and application of RPA primer and kit

Abstract

The invention discloses an RPA (recombinase polymerase amplification) primer and a kit for rapidly detecting citrus huanglongbing Asian species as well as a detection method and application of the RPAprimer. The RPA amplification primer HLBa-F / HLBa-R developed on the basis of the conserved sequence 16S rDNA of the citrus huanglongbing Asian species establishes an RPA detection method for detecting the citrus huanglongbing Asian species for the first time, effectively eliminates false positive interference, is short in detection time consumption, economical and applicable, does not need expensive instruments and equipment such as a PCR instrument and a fluorescent quantitative PCR instrument, and is simple and easy to learn; powerful technical support can be provided for early detection ofcitrus yellow shoot of disease-free citrus seedlings in China, detection and confirmation of suspected citrus yellow shoot trees in fields and popularization of the detection capability of grass-roots scientific research units, so healthy development of disease-free citrus seedling breeding in China is promoted, spreading and diffusion of citrus yellow shoot in wide citrus production areas are early warned in advance, and the early warning effect is achieved; sustainable development of the citrus industry in China is further promoted.

Description

technical field [0001] The invention relates to a detection technology of plant quarantine diseases in my country, in particular to an RPA primer, a kit, a detection method and application thereof for rapidly detecting citrus huanglongbing Asian species. Background technique [0002] Citrus Huanglongbing (Huanglongbing) is a worldwide destructive disease, which has the characteristics of strong outbreak, rapid development, and great harm. It is listed as a quarantine disease in my country. Citrus huanglongbing is spreading in citrus growing areas all over the world, such as Asia, Africa, America and other regions. Citrus greening disease has a long duration, wide range and high incidence rate, resulting in short lifespan, low yield, high production cost and heavy economic losses of citrus in some areas of my country, which seriously restricts the healthy development of my country's citrus industry. In 2017, the area of ​​citrus Huanglongbing disease in the 10 provinces (dis...

AI Technical Summary

Problems solved by technology

At present, RPA technology has been used in the rapid diagnosis of medical pathogens, and there are some reports on the detection of plant pathogens, but there is no report on the detection of citrus Huanglongbing pathogens by RPA technology.

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