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Tissue culture method of gerbera jamesonii

A technology of tissue culture and gerbera, which is applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient and long induction cycle, etc., and achieve the effects of increasing the reproduction coefficient, fast multiplication speed, and shortening the seedling cycle

Inactive Publication Date: 2021-02-02
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, papers or patents on the establishment of the tissue culture rapid propagation technology system of gerbera have been published successively, but there are generally problems such as long induction period and low reproduction coefficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Establishment of Regeneration System of Cut Gerbera Variety 'Hongyun 6'

[0049] (1) Acquisition of explants: collect 20 tender flower buds (0.6 cm in diameter) of the gerbera cut flower variety 'Hongyun No. h, spare.

[0050] (2) Disinfection of explants: use 75% alcohol to clean the receptacle for 40 seconds on the ultra-clean table, and then use 0.1% HgCl 2 Treat for 12 minutes, wash with sterile water 5 times, remove the bracts and tubular first flowers, cut the receptacle into 3mm×3mm slices, and set aside.

[0051] (3) Induction of sterile seedlings: Inoculate the receptacle thin slices directly on the bud induction medium in a flat manner. The components of the medium are: sucrose 30g / L+agar 7.2g / L+MS+2.8mg / L benzene Thiadiazolyl urea (TDZ)+0.8mg / L NAA+1.0mg / L multi-walled carbon nanotubes. The culture temperature is 25±1℃, and the light intensity is 250μmol·m -2 ·s -1 , Lighting time 16h. 33 days after inoculation, there were green protrusions on the recep...

Embodiment 2

[0061] Establishment of regeneration system of cut gerbera cultivar 'Qiuyun 3'

[0062] (1) Acquisition of explants: Collect 15 young flower buds (0.8 cm in diameter) of the gerbera cut flower variety 'Qiu Yun No. h, spare.

[0063] (2) Disinfection of explants: use 75% alcohol to clean the receptacle for 42 seconds on the ultra-clean table, and then use 0.1% HgCl 2 Treat for 14 minutes, wash with sterile water 5 times, remove the bracts and tubular first flowers, cut the receptacle into 3mm×3mm slices, and set aside.

[0064] (3) Induction of sterile seedlings: Inoculate the receptacle slices directly onto the bud induction medium in a flat manner. The components of the medium are: sucrose 30g / L+agar 7.2g / L+MS+2.6mg / L benzene Thiadiazolyl urea (TDZ)+0.6mg / L NAA+1.0mg / L multi-wall carboxylated carbon nanotubes. The culture temperature is 25±1℃, and the light intensity is 100~300μmol·m -2 ·s -1 , Lighting time 16h. 35 days after inoculation, there were green protrusions o...

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Abstract

The invention provides a tissue culture method of gerbera jamesonii, and belongs to the technical field of plant tissue culture. The method comprises the following steps of 1) carrying out callus culture and subculture multiplication culture on sterile gerbera jamesonii leaves with petioles to obtain calluses; and 2) carrying out differentiation culture and subculture on the calluses obtained in the step 1) to obtain gerbera jamesonii clustered seedlings. By adopting the tissue culture method provided by the invention, the callus induction rate is high, the propagation speed is high, the callus differentiation efficiency is high, the number of cluster buds is large, the bud growth speed is high, the propagation coefficient of gerbera jamesonii can be greatly increased, the propagation speed is increased, the seedling period is shortened, and large-scale cultivation and cut flower production of the gerbera jamesonii are greatly facilitated.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method of gerbera. Background technique [0002] Gerbera (Gerbera jamesonii Bolus), also known as Gerbera, is a perennial herb of the genus Gerbera in the family Asteraceae. Native to the Transvaal in southern Africa, it likes a warm, ventilated and sunny environment. Because of its rich flower colors, large flowers and bright colors, it can be used for cut flowers, potted plants and garden decorations. Especially as cut flowers, it is loved by many consumers and is one of the top five fresh cut flowers in the world. At present, it has been widely cultivated all over the world. [0003] The propagation of gerbera seedlings is mainly based on tissue culture rapid propagation. The specific method is to use the receptacle as an explant to induce a small amount of callus. The callus does not undergo proliferation induction, and directly d...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 李绅崇王继华杨春梅卢珍红单芹丽汪国鲜莫锡君吴丽芳郑玉红
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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