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Special detection mask and method for Raman immune probe for respiratory virus collection and detection

A detection method and respiratory technology, applied in Raman scattering, application, special clothing, etc., can solve the problems of low positive rate of throat swab paper detection, reduce the risk of infection, facilitate work pressure, and reduce sampling and detection The effect of pressure

Inactive Publication Date: 2021-02-05
ZHEJIANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of lower respiratory tract samples requires specialized equipment and specialized technicians
Previous studies have shown that the positive rate of sputum samples from patients diagnosed by RT-PCR is 74.4%-88.9%; the positive rate of nasal swab samples is 53.6%-73.3%; Low

Method used

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  • Special detection mask and method for Raman immune probe for respiratory virus collection and detection
  • Special detection mask and method for Raman immune probe for respiratory virus collection and detection
  • Special detection mask and method for Raman immune probe for respiratory virus collection and detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Preparation of gold nanoparticles

[0043] Take 2.2mM 150mL sodium citrate and heat it in a 250mL three-neck flask. After boiling, add 1 mL of 25 mM HAuCl 4 , the color of the solution turned pink within 10 min. This solution is used as a seed solution. In the same reaction vessel, add 1mL of 25mM HAuCl after the temperature of the solution drops to 90°C 4 . The reaction was completed after 20.30 min of aqueous solution. This process was repeated 2 times. Thereafter, 55 mL of the reaction solution was taken out and 53 mL of ultrapure water and 2 mL of 60 mM sodium citrate were added. This solution was then used as a seed solution to carry out the above reaction, which was repeated several times to obtain gold nanoparticles (GNPs) of different sizes.

[0044] 2) Preparation of Raman-labeled immunoprobes

[0045] Take 10 mL of the above-prepared gold nanoparticles, adjust the pH to 9.0 with 0.1 M potassium carbonate, then add 500 μL of carbonate buffer solution ...

Embodiment 2

[0051] Example 2 Detection of Novel Coronavirus Raman Spectroscopy Standard Item, Blank and Sample

[0052] Take the nucleocapsid protein standard of the new coronavirus and drop it on the non-woven fabric of the prepared dry gold nanoparticles (Raman immunoprobe), and wait for it to dry naturally. The above-mentioned Raman probe bound to the nucleocapsid protein is used for Raman spectrum detection to obtain a spectrum of a standard product.

[0053] Put the prepared dry gold nanoparticles (Raman immunoprobe) on the non-woven fabric, and use the gold nanoparticles with only Raman probes for Raman spectrum detection to obtain a blank substrate spectrum.

[0054] Take the mask worn by the suspected novel coronavirus infection for 2-8 hours, and take out the attachment of the Raman spectrum immune probe for Raman spectrum detection. Compare the spectrum of the detection peak with the base spectrum of the blank and the spectrum of the standard. If the spectrum of the detection p...

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PUM

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Abstract

The invention discloses a special detection mask and method for a Raman immune probe for respiratory virus collection and detection. An area or an accessory sprayed with a Raman immune probe is attached to the melt-blown cloth layer on the inner side of the mask, and the area or the accessory is located at the position right corresponding to the mouth and the nose; the Raman immune probe adsorbs aRaman marker and a monoclonal antibody or a polyclonal antibody aiming at respiratory viruses, a solution of the Raman immune probe is directly sprayed on a melt-blown cloth layer or an accessory inthe middle of the mask, and an openable position is arranged on the inner side of the mask. The accessory sprayed with the Raman immune probe is detachably arranged at the openable position through anon-woven fabric combined with a magic tape, and the Raman immune probe is obtained by a special preparation method.

Description

technical field [0001] The invention relates to a mask and a detection method in the technical field of detection of respiratory pathogens, in particular to a special detection mask and method of a Raman immune probe used for collection and detection of respiratory viruses. Background technique [0002] Novel coronavirus pneumonia is an infectious disease caused by infection with the new coronavirus SARS-CoV-2. At present, the top priority of epidemic prevention and control is to accurately identify virus-infected patients, especially mild and asymptomatic patients, who need to be isolated in time. The diagnostic method of the disease is mainly in the detection of nasopharyngeal swabs, sputum, alveolar lavage fluid and other specimens. [0003] Detection of viral nucleic acid. Studies have shown that SARS-Cov-2 mainly infects the lower respiratory tract, detected through nasopharyngeal swabs and bronchial samples. However, the collection of lower respiratory tract samples...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A41D13/11A41D31/02A41D31/04G01N33/577G01N33/569G01N21/65
CPCA41D13/1115A41D31/02A41D31/04G01N33/577G01N33/56983G01N21/658G01N2333/165
Inventor 陆绍红丁豪杰吕杭军丁建组孔庆明郑斌楼涤
Owner ZHEJIANG MEDICAL COLLEGE
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