Method for controlling chondroitin sulfate content by adjusting feed liquid conductivity

A technology of chondroitin sulfate and electrical conductivity, which is applied in the field of chemical technology, can solve the problems of increasing remanufacturing costs, occupying environmental protection resources, and general quality level, and achieves the effects of improving product yield, saving environmental protection resources, and saving process time

Inactive Publication Date: 2021-02-05
MEITEK TECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the existing technology basically adopts the process of hydrogen peroxide oxidation to remove impurity protein, the removal effect of impurity protein is relatively poor, resulting in the following problems: 1) the content of the finished chondroitin produced is generally low, and the general effective content is about 90%, <95% , average quality level, low added value
[0004] 2) In the production process, the cost of adjusting the content of chondroitin is relatively high. For example, some production enterprises pursuing high quality use polymer membrane separation system to repeatedly wash the material to purify the prod

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024]Example 1

[0025]1) Prepare the chondroitin liquid, take 1kg of bovine cartilage, add animal cartilage to 3 times the weight of drinking water, raise the temperature to 85℃, cook for 5h, drain and cool down, reduce the temperature to 55℃, adjust the pH To 9, add 0.3% of the total amount of the feed solution with biological complex enzymes for enzymatic hydrolysis (the complex enzymes include neutral enzymes and alkaline enzymes), and the enzymatic hydrolysis time is 10 hours. After inactivation, the enzymatic hydrolysis solution is clarified and filtered, and the material solution is taken for Ultrafiltration concentrated 2 times;

[0026]2) Adjust the electrical conductivity, add an appropriate amount of sodium chloride to the chondroitin material solution, so that the electrical conductivity of the chondroitin material solution reaches 100ms / cm;

[0027]3) Alcohol precipitation: After adjusting, slowly add 95% ethanol to the material liquid to adjust the alcohol content to 50%, and ...

Example Embodiment

[0029]Example 2

[0030]1) Prepare the chondroitin liquid, take 1kg of bovine cartilage, add animal cartilage to 3 times the weight of drinking water, raise the temperature to 85℃, cook for 5h, drain and cool down, reduce the temperature to 55℃, adjust the pH To 9, add 0.3% of the total amount of the feed solution with biological complex enzymes for enzymatic hydrolysis (the complex enzymes include neutral enzymes and alkaline enzymes), and the enzymatic hydrolysis time is 10 hours. After inactivation, the enzymatic hydrolysis solution is clarified and filtered, and the material solution is taken for Concentrated by ultrafiltration 3 times;

[0031]2) Adjust the electrical conductivity, add appropriate amount of sodium chloride to the chondroitin material solution, so that the electrical conductivity of the chondroitin material solution can reach 150ms / cm;

[0032]3) Alcohol precipitation: After the adjustment is completed, slowly add 95% ethanol to the material solution to adjust the alcoho...

Example Embodiment

[0034]Example 3

[0035]1) Prepare the chondroitin feed solution, take 1kg of bovine cartilage, add animal cartilage to 20kg of drinking water, add 1.5% sodium hydroxide of the total amount of the material, soak and extract for 30h, perform solid-liquid separation and add, then heat the feed liquid To 55 degrees Celsius, adjust the pH to 9, add 0.2% of the total amount of the feed solution with biological complex enzymes for enzymatic hydrolysis (the complex enzymes include neutral enzymes and alkaline enzymes). The enzymatic hydrolysis time is 3 hours. After inactivation, the enzymatic hydrolysis solution is carried out After clarification and filtration, the taken liquid is concentrated 4 times by ultrafiltration;

[0036]2) Adjust the electrical conductivity, add appropriate amount of potassium chloride to the chondroitin material solution, so that the electrical conductivity of the chondroitin material solution reaches 50ms / cm;

[0037]3) Alcohol precipitation: After adjusting, slowly ad...

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Abstract

The invention discloses a method for controlling chondroitin sulfate content by regulating feed liquid conductivity. The method comprises the following steps: preparing chondroitin sulfate feed liquidby an alkali extraction method or full enzymolysis, clarifying and filtering the chondroitin sulfate feed liquid, concentrating, regulating the conductivity, precipitating with ethanol, separating, washing with ethanol, separating and drying, preparing the chondroitin sulfate finished produc, and detecting that the content of chondroitin sulfate in the chondroitin sulfate finished product reaches99.7%. The content of chondroitin sulfate in the chondroitin sulfate finished product is controlled by adjusting the conductivity of the feed liquid, the one-time production content of the chondroitin sulfate finished product can reach 95% or above, a membrane separation repeated washing production mode is not needed, and product yield is increased. A large amount of process time is saved, a large amount of wastewater is not generated, and environment-friendly resources of production enterprises are saved.

Description

technical field [0001] The invention belongs to the technical field of chemical engineering, and in particular relates to a method for controlling the content of chondroitin sulfate by adjusting the conductivity of feed liquid. Background technique [0002] Chondroitin sulfate is a kind of glycosaminoglycan, which belongs to acidic mucopolysaccharide, and its molecular weight is generally 20-50KDa. It is widely used in the field of dietary supplements and has a significant effect on joint maintenance and elimination of inflammation. [0003] At present, the industrial production of chondroitin is basically an alcohol precipitation process. In the production process, ethanol is used for precipitation and dehydration of chondroitin, and then drying equipment is used for drying and subsequent finishing of chondroitin. Because the existing technology basically adopts the process of hydrogen peroxide oxidation to remove impurity protein, the removal effect of impurity protein is ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069C08B37/0003
Inventor 张亮黄周金于明晓赵伟学宋彦卓姚立兵
Owner MEITEK TECH QINGDAO
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