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Method for controlling chondroitin sulfate content by adjusting feed liquid conductivity

A technology of chondroitin sulfate and electrical conductivity, which is applied in the field of chemical technology, can solve the problems of increasing remanufacturing costs, occupying environmental protection resources, and general quality level, and achieves the effects of improving product yield, saving environmental protection resources, and saving process time

Inactive Publication Date: 2021-02-05
MEITEK TECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the existing technology basically adopts the process of hydrogen peroxide oxidation to remove impurity protein, the removal effect of impurity protein is relatively poor, resulting in the following problems: 1) the content of the finished chondroitin produced is generally low, and the general effective content is about 90%, <95% , average quality level, low added value
[0004] 2) In the production process, the cost of adjusting the content of chondroitin is relatively high. For example, some production enterprises pursuing high quality use polymer membrane separation system to repeatedly wash the material to purify the product, which requires not only a large amount of equipment Investment requires the replacement of membrane modules every year. Repeated washing also causes a lot of sewage to be treated, which takes up a lot of environmental protection resources and causes waste.
There are also some manufacturers that increase the content of chondroitin by refining the crude chondroitin again. The method used is to dissolve the crude product, and then add neutral salt to carry out alcohol precipitation and drying again, but this method will reduce the yield of chondroitin. The reduction, and increase the cost of remanufacturing, low economic benefit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Prepare the chondroitin material liquid, take 1kg of bovine cartilage, add animal cartilage to drinking water with 3 times the weight, increase the temperature to 85°C, cook for 5 hours, cool down after draining, reduce the temperature to 55°C, and adjust the pH To 9, add 0.3% biological compound enzyme in the total amount of feed liquid for enzymolysis (complex enzyme includes neutral enzyme and alkaline enzyme), and the enzymolysis time is 10h. After inactivation, the enzymolysis solution is clarified and filtered, and the feed solution is taken for Concentrated by ultrafiltration 2 times;

[0026] 2) Regulating the conductivity, adding an appropriate amount of sodium chloride to the chondroitin feed solution to make the conductivity of the chondroitin feed solution reach 100ms / cm;

[0027] 3) Alcohol precipitation: After the adjustment is completed, slowly add 95% ethanol to the feed liquid to adjust the alcohol content to 50%, and then directly enter the suspensi...

Embodiment 2

[0030] 1) Prepare the chondroitin material liquid, take 1kg of bovine cartilage, add animal cartilage to drinking water with 3 times the weight, increase the temperature to 85°C, cook for 5 hours, cool down after draining, reduce the temperature to 55°C, and adjust the pH To 9, add 0.3% biological compound enzyme in the total amount of feed liquid for enzymolysis (complex enzyme includes neutral enzyme and alkaline enzyme), and the enzymolysis time is 10h. After inactivation, the enzymolysis solution is clarified and filtered, and the feed solution is taken for Concentrated by ultrafiltration 3 times;

[0031] 2) Regulating the conductivity, adding an appropriate amount of sodium chloride to the chondroitin feed solution to make the conductivity of the chondroitin feed solution reach 150ms / cm;

[0032] 3) Alcohol precipitation: After the adjustment is completed, slowly add 95% ethanol to the feed liquid to adjust the alcohol content to 65%, and then directly enter the suspensi...

Embodiment 3

[0035] 1) To prepare the chondroitin feed solution, take 1 kg of bovine cartilage, add the animal cartilage to 20 kg of drinking water, add 1.5% sodium hydroxide of the total amount of the material, soak and extract for 30 hours, perform solid-liquid separation and addition, and then raise the temperature of the feed liquid To 55 degrees Celsius, adjust the pH to 9, add 0.2% of the total amount of feed liquid biological complex enzymes for enzymolysis (complex enzymes include neutral enzymes, alkaline enzymes), enzymolysis time is 3h, after inactivation, the enzymolysis solution After clarification and filtration, the feed liquid is concentrated by ultrafiltration to 4 times;

[0036] 2) Regulating the conductivity, adding an appropriate amount of potassium chloride to the chondroitin feed solution to make the conductivity of the chondroitin feed solution reach 50ms / cm;

[0037] 3) Alcohol precipitation: After the adjustment is completed, slowly add 95% ethanol to the feed liq...

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Abstract

The invention discloses a method for controlling chondroitin sulfate content by regulating feed liquid conductivity. The method comprises the following steps: preparing chondroitin sulfate feed liquidby an alkali extraction method or full enzymolysis, clarifying and filtering the chondroitin sulfate feed liquid, concentrating, regulating the conductivity, precipitating with ethanol, separating, washing with ethanol, separating and drying, preparing the chondroitin sulfate finished produc, and detecting that the content of chondroitin sulfate in the chondroitin sulfate finished product reaches99.7%. The content of chondroitin sulfate in the chondroitin sulfate finished product is controlled by adjusting the conductivity of the feed liquid, the one-time production content of the chondroitin sulfate finished product can reach 95% or above, a membrane separation repeated washing production mode is not needed, and product yield is increased. A large amount of process time is saved, a large amount of wastewater is not generated, and environment-friendly resources of production enterprises are saved.

Description

technical field [0001] The invention belongs to the technical field of chemical engineering, and in particular relates to a method for controlling the content of chondroitin sulfate by adjusting the conductivity of feed liquid. Background technique [0002] Chondroitin sulfate is a kind of glycosaminoglycan, which belongs to acidic mucopolysaccharide, and its molecular weight is generally 20-50KDa. It is widely used in the field of dietary supplements and has a significant effect on joint maintenance and elimination of inflammation. [0003] At present, the industrial production of chondroitin is basically an alcohol precipitation process. In the production process, ethanol is used for precipitation and dehydration of chondroitin, and then drying equipment is used for drying and subsequent finishing of chondroitin. Because the existing technology basically adopts the process of hydrogen peroxide oxidation to remove impurity protein, the removal effect of impurity protein is ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069C08B37/0003
Inventor 张亮黄周金于明晓赵伟学宋彦卓姚立兵
Owner MEITEK TECH QINGDAO
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