Method for controlling chondroitin sulfate content by adjusting feed liquid conductivity
A technology of chondroitin sulfate and electrical conductivity, which is applied in the field of chemical technology, can solve the problems of increasing remanufacturing costs, occupying environmental protection resources, and general quality level, and achieves the effects of improving product yield, saving environmental protection resources, and saving process time
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[0024]Example 1
[0025]1) Prepare the chondroitin liquid, take 1kg of bovine cartilage, add animal cartilage to 3 times the weight of drinking water, raise the temperature to 85℃, cook for 5h, drain and cool down, reduce the temperature to 55℃, adjust the pH To 9, add 0.3% of the total amount of the feed solution with biological complex enzymes for enzymatic hydrolysis (the complex enzymes include neutral enzymes and alkaline enzymes), and the enzymatic hydrolysis time is 10 hours. After inactivation, the enzymatic hydrolysis solution is clarified and filtered, and the material solution is taken for Ultrafiltration concentrated 2 times;
[0026]2) Adjust the electrical conductivity, add an appropriate amount of sodium chloride to the chondroitin material solution, so that the electrical conductivity of the chondroitin material solution reaches 100ms / cm;
[0027]3) Alcohol precipitation: After adjusting, slowly add 95% ethanol to the material liquid to adjust the alcohol content to 50%, and ...
Example Embodiment
[0029]Example 2
[0030]1) Prepare the chondroitin liquid, take 1kg of bovine cartilage, add animal cartilage to 3 times the weight of drinking water, raise the temperature to 85℃, cook for 5h, drain and cool down, reduce the temperature to 55℃, adjust the pH To 9, add 0.3% of the total amount of the feed solution with biological complex enzymes for enzymatic hydrolysis (the complex enzymes include neutral enzymes and alkaline enzymes), and the enzymatic hydrolysis time is 10 hours. After inactivation, the enzymatic hydrolysis solution is clarified and filtered, and the material solution is taken for Concentrated by ultrafiltration 3 times;
[0031]2) Adjust the electrical conductivity, add appropriate amount of sodium chloride to the chondroitin material solution, so that the electrical conductivity of the chondroitin material solution can reach 150ms / cm;
[0032]3) Alcohol precipitation: After the adjustment is completed, slowly add 95% ethanol to the material solution to adjust the alcoho...
Example Embodiment
[0034]Example 3
[0035]1) Prepare the chondroitin feed solution, take 1kg of bovine cartilage, add animal cartilage to 20kg of drinking water, add 1.5% sodium hydroxide of the total amount of the material, soak and extract for 30h, perform solid-liquid separation and add, then heat the feed liquid To 55 degrees Celsius, adjust the pH to 9, add 0.2% of the total amount of the feed solution with biological complex enzymes for enzymatic hydrolysis (the complex enzymes include neutral enzymes and alkaline enzymes). The enzymatic hydrolysis time is 3 hours. After inactivation, the enzymatic hydrolysis solution is carried out After clarification and filtration, the taken liquid is concentrated 4 times by ultrafiltration;
[0036]2) Adjust the electrical conductivity, add appropriate amount of potassium chloride to the chondroitin material solution, so that the electrical conductivity of the chondroitin material solution reaches 50ms / cm;
[0037]3) Alcohol precipitation: After adjusting, slowly ad...
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