One-way negative pressure adsorption sampling and filtering device and method

Abstract

The invention provides a one-way negative-pressure adsorption sampling and filtering device and method. The device comprises a liquid taking tube, a filter and a negative-pressure metering bottle, wherein an oblique puncture port is formed in the liquid inlet end of the liquid taking tube, and a one-way valve is connected to the liquid outlet end of the liquid taking tube; the liquid inlet end ofthe filter is connected with the one-way valve, and the liquid outlet end of the filter is connected with a stop valve; the liquid inlet end of the negative-pressure metering bottle is connected withthe stop valve through a connecting pipe; the liquid outlet end of the negative-pressure metering bottle is connected with a negative-pressure vacuum pump through an air guide pipe; and a filter cavity is formed in the filter and is filled with a filter medium. According to the device, the operation process is simplified, and the operation safety is improved. The filter medium adopted by the method is a microporous filter membrane, and the specific operation process comprises the following steps: instrument assembling, disinfecting, sterile puncture drainage, control for the negative-pressureflow, quantitative adsorbing and filtering, prepared sample storage and transportation, cleaning area filter membrane transferring and transferring for biochemical culture; and water sample collectionand adsorption are directly carried out on a water quality sampling site to prepare a microporous filter membrane sample, so that the purpose of real-time, convenient, rapid and pollution-free samplepreparation is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological and chemical filtration sample preparation, and in particular relates to a one-way negative pressure adsorption sampling filtration device and method. Background technique [0002] In the field of daily biochemical analysis, the preparation of reagents, standard solutions and filtration preparation of liquid samples are often used to achieve the purposes of impurity removal, homogenization, purification and sterilization. Impurity removal refers to the removal of larger particles, crystals, gels and flocs in the solution by filtration; homogenization refers to the use of filtration to separate the dispersed particles in the suspension and emulsion system, reduce the size of the dispersed and improve Uniform distribution of dispersed matter; purification refers to the removal of tiny particles, plankton, mineral rocks, heavy metal ions, etc. through membrane filtration; sterilization uses the fi...

AI Technical Summary

Problems solved by technology

However, the filtration equipment currently used is relatively large and lacks matching disinfection equipment

Moreover, since the collection and preparation of water quality samples are carried out in stages, the inspection time period is naturally prolonged, which easily increases the risk of cross-contamination, and the procedures of disinfection, sampling, transfer, and filtration are cumbersome and delicate, requiring high requirements for inspectors and high work intensity. Large, delaying work efficiency

[0006] In the traditional method of sample preparation, the operation process of sampling, transfer, and membrane preparation is long, the clean environment and space are relatively spacious, and the collection container needs to be thoroughly disinfected, otherwise the target microorganisms are easily affected by the water storage container, sampling environment, and transportation conditions. There will be uncertainties of secondary pollution, such as media contact such as transport and storage materials, outdoor air, and multiple transfers

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