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Preparation method of microbubble preparation for ultrasonic diagnosis and treatment

An ultrasonic diagnosis and microbubble technology, applied in the field of tumor treatment, can solve problems such as unsatisfactory action spectrum, large toxicity and side effects of sound sensitizers, single components, etc., and achieve the stage of reducing gradient power. Ultrasonic response performance, the effect of improving the preparation effect

Inactive Publication Date: 2021-02-12
THE AFFILIATED HOSPITAL OF GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used clinical sensitizers have unstable composition ratios, slow excretion, and are prone to phototoxic side effects. Skin tests are required before medication, and light should be kept away from light for 1 month after medication. In addition, their action spectrum is not ideal, and this type of sensitive substance is not specific. Strong substances and other factors have seriously affected the actual effect of sonodynamic therapy and its clinical application. Clinically, chlorin, phthalocyanine, etc. are commonly used in clinical research and have a single component and good performance. The second-generation photosensitizer, the third-generation photosensitizer with recognition function
[0004] However, most of the existing sonosensitizers that can be used clinically or in research still have problems such as large toxic and side effects and unstable components, which seriously hinders the further development of sonodynamic therapy. Sonosensitizers for sonodynamic therapy to solve and optimize these existing problems

Method used

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  • Preparation method of microbubble preparation for ultrasonic diagnosis and treatment
  • Preparation method of microbubble preparation for ultrasonic diagnosis and treatment
  • Preparation method of microbubble preparation for ultrasonic diagnosis and treatment

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Embodiment 1

[0051] A method for preparing a microbubble preparation for ultrasonic diagnosis and treatment, the microbubble preparation is composed of a shell membrane layer and an internal cavity, comprising the following steps:

[0052] S1: Synthesis of metalloporphyrin complexes by chemical synthesis, the metalloporphyrin complexes are metalloporphyrin complexes (formula 1) with Ti as the central atom, such as figure 1 As shown, the metalloporphyrin complex was added into the albumin solution for ultrasonic treatment to obtain the albumin-wrapped metalloporphyrin complex, wherein the mass ratio of the metalloporphyrin complex to albumin was 1:80, ready to use ;

[0053] S2: Select the metalloporphyrin complex obtained in step S1, and proportion the components as follows in parts by weight: 37 parts of metalloporphyrin complex, 125 parts of phospholipid, 20 parts of saturated fatty acid, mix and disperse In an organic solvent of 40 times its volume, a mixed solution is obtained; wherei...

Embodiment 2

[0076] This embodiment is basically the same as Embodiment 1, and the difference is that the number of components of the mixed solution in step S2 is different, specifically:

[0077] S2: Select the metalloporphyrin complex obtained in step S1, and proportion the components in parts by weight as follows: 20 parts of metalloporphyrin complex, 50 parts of phospholipid, and 15 parts of saturated fatty acid, mix and disperse In an organic solvent of 20 times its volume, a mixed solution is obtained; wherein, the organic solvent is selected from acetone, and the phospholipid is selected from a mixture of phosphatidylglycerol and diphosphatidylglycerol in a mass ratio of 1:2; the Saturated fatty acids are selected from palmitic acid.

Embodiment 3

[0079] This embodiment is basically the same as Embodiment 1, and the difference is that the number of components of the mixed solution in step S2 is different, specifically:

[0080] S2: Select the metalloporphyrin complex obtained in step S1, and proportion the components in parts by weight as follows: 45 parts of metalloporphyrin complex, 150 parts of phospholipid, and 25 parts of saturated fatty acid, mix and disperse In an organic solvent of 50 times its volume, a mixed solution is obtained; wherein, the organic solvent is selected from acetone, and the phospholipid is selected from a mixture of phosphatidylglycerol and diphosphatidylglycerol in a mass ratio of 1:2; the Saturated fatty acids are selected from palmitic acid.

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Abstract

The invention discloses a preparation method of a microbubble preparation for ultrasonic diagnosis and treatment, the microbubble preparation is composed of a shell membrane layer and an internal cavity, and the preparation method comprises the following steps: S1, synthesizing a metalloporphyrin complex through a chemical synthesis method for later use; S2, selecting the metalloporphyrin complexobtained in the step S1, mixing the following components in parts by weight: 20-45 parts of metalloporphyrin complex, 50-150 parts of phospholipid and 15-25 parts of saturated fatty acid, and dispersing the mixture into an organic solvent of which the volume is 20-50 times that of the mixture to obtain a mixed solution; and S3, filling a microbubble preparation generator with the mixed solution obtained in the step S2, and then carrying out gradient power stage complex wave ultrasonic dispersion treatment at the constant temperature of 35-50 DEG C to form a suspension, and S3, filling the suspension with gas at the stage III in the step S3, and then carrying out rotary oscillation treatment for 1-5 minutes to obtain the microbubble preparation. Compared with a traditional sound-sensitive agent, the microbubble preparation provided by the invention has lower toxic and side effects and stronger ultrasonic response performance.

Description

technical field [0001] The invention relates to the technical field of tumor treatment, in particular to a preparation method of a microbubble preparation for ultrasonic diagnosis and treatment. Background technique [0002] Sonodynamic therapy uses ultrasonic waves to have a strong penetrating ability to biological tissues, especially to gather ultrasonic energy to non-invasively focus sound energy on deep tissues, and activate some sound-sensitive drugs to produce anti-tumor effects. [0003] The choice of sonosensitizer has become the most critical issue in the field of sonodynamic therapy. Commonly used clinical sensitizers have unstable composition ratios, slow excretion, and are prone to phototoxic side effects. Skin tests are required before medication, and light should be kept away from light for 1 month after medication. In addition, their action spectrum is not ideal, and this type of sensitive substance is not specific. Strong substances and other factors have se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K9/50A61K41/00A61K47/24A61K47/42A61P35/00
CPCA61K49/223A61K41/0033A61K47/24A61K9/5052A61P35/00
Inventor 黄智赵许亚张帅
Owner THE AFFILIATED HOSPITAL OF GUIZHOU MEDICAL UNIV