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Method for quickly detecting cryptococcus gatticus

A technology for Cryptococcus neoformans and cocci, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc. It can solve the problems of cumbersome operation, high error rate, and low sensitivity, and achieve simple operation and applicable Strong, sensitive effects

Inactive Publication Date: 2021-02-12
北京量觉科技有限责任公司
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AI Technical Summary

Problems solved by technology

[0007] In order to overcome the defects of traditional detection of Cryptococcus gattii, such as complicated operation, low sensitivity, and high error rate, the present invention provides a method for accurately detecting Cryptococcus gattii, which can be used for diagnostic purposes or non-diagnostic purposes

Method used

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  • Method for quickly detecting cryptococcus gatticus
  • Method for quickly detecting cryptococcus gatticus
  • Method for quickly detecting cryptococcus gatticus

Examples

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Embodiment 1

[0033] Target genes, primers, probes and kits for detection and identification of Cryptococcus neoformans and Cryptococcus gattii

[0034] Dozens of genome sequences of Cryptococcus neoformans and Cryptococcus gattii were obtained through high-throughput sequencing technology, and the genome sequence differences of dozens of Cryptococcus were compared by genomics methods, and the CAP59 sequence was selected as the target gene. Cryptococcus is relatively conserved within the species, and there are differences between Cryptococcus neoformans and Cryptococcus gattii. The nucleotide sequences of target genes for detecting Cryptococcus neoformans and Cryptococcus gattii are shown in SEQ ID NO:5 and SEQ ID NO:6.

[0035] Primer software was used to design primers for the selected target gene sequence information. Through repeated comparative analysis, real-time fluorescent PCR detection primers and probes that can be used for the detection and identification of Cryptococcus neoform...

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Abstract

The invention belongs to the technical field of fungal etiology detection, and discloses a method for quickly detecting cryptococcus gattii. The method comprises the following steps: 1) selecting a sample to be detected; (2) extracting DNA of cryptococcus gattii; (3) preparing a real-time fluorescent PCR reaction system; (4) conducting fluorescent PCR amplification. The method can quickly and accurately detect cryptococcus gattii, and sensitivity and specificity are high.

Description

technical field [0001] The invention belongs to the technical field of fungal etiology detection, and in particular relates to a method for accurately detecting cryptococcus gattii. Background technique [0002] Cryptococcus is a germ that causes cryptococcosis. The medium is usually dried pigeon droppings, and there are few reports of human-to-human transmission. There is no specific drug yet, and symptomatic treatment is generally carried out. Cryptococcosis is a pulmonary or disseminated infectious disease caused by Cryptococcus bacteria, mainly causing pneumonia and meningitis, but also causing skin, bone or internal organ infections. Clinically, the diagnosis is made based on clinical manifestations and microscopic examination results, and then confirmed by fungal culture or tissue staining. Clinical treatment drugs include amphotericin B, triazole antifungal drugs and flucytosine. [0003] There are 37 species of Cryptococcus currently identified, among which Crypt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2561/113C12Q2563/107
Inventor 薛新颖刘玉霞赵晟臧学磊
Owner 北京量觉科技有限责任公司
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