A kind of breeding method of silkworm resistant to densokaryophysis

Abstract

The invention discloses a method for breeding silkworms resistant to dense nuclear disease. F1 generation: determine the Bmnsd-Z target point and construct sgRNA-containing plasmid pXL[IE1-DsRed-U6-sgRNA], and introduce it into the silkworm; The F1 generation with the plasmid is self-crossed, the red fluorescent individuals are screened, and the positive individuals of the F2 generation are obtained; the F3 generation: the F2 generation is crossed with the silkworm expressing the Cas9 protein to obtain double fluorescent offspring, and the initial screening is carried out for the resistance to densokaryophyllosis and genome identification. Determine the positive individuals of the F3 generation; F4 generation: self-select the non-fluorescent individuals of the F3 generation, and then carry out the identification of the genome and disease resistance again, and determine the non-fluorescent target strain with the ability to resist dense nuclear disease; F5 generation: selected from the F4 generation The target line is crossed with the Js variety, and the resistance gene is introduced into the recipient variety for breeding. The silkworm that is resistant to dense nuclear disease and can be used for production has been bred.

Description

technical field [0001] The invention relates to the selection and breeding of silkworm disease-resistant strains, in particular to a breeding method for silkworms resistant to dense nuclear disease. Background technique [0002] The silkworm is a representative Lepidoptera insect and is widely used as a biological model for research. In the traditional silk textile industry, the silkworm has the characteristics of high-quality silk, short life cycle and easy reproduction. Sericulture is still one of the pillar industries in many rural areas, but because silkworm diseases seriously threaten sericulture production, it will cause 20% of the total sericulture output every year. Economic losses, including bacteria, fungi, and viral diseases, dense nuclear Disease is one of them. [0003] Densoplasmosis is one of the main diseases affecting sericulture production in recent years. The incubation period of silkworms infected with densoplasmosis is usually about 7 days. The main sy...

AI Technical Summary

Problems solved by technology

In addition, the traditional conventional breeding method can effectively resist the damage of the virus to the silkworm, but due to the slow effect of the traditional method, the long breeding cycle, and the workload greatly limit the efficiency of its breeding, gene editing technology is currently more rapid. anti-disease technology

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