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Method for determining carbon isotope content of soil microorganisms

A technology of soil microorganisms and carbon isotopes, which is applied in the field of soil microbial carbon measurement, can solve the problems of large error in detection results, complex technology, and undetectable results, and achieve the effects of increasing content, high sensitivity, and reducing volume

Pending Publication Date: 2021-02-19
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the carbon isotope determination of soil microorganisms requires the use of high-precision instruments, the technology is more complicated, and the cost of a single measurement is relatively expensive
Soil microbial carbon extraction is mainly through "chloroform fumigation-K 2 SO 4 Extraction” is realized, because the obtained liquid sample after extraction, the injection concentration often does not meet the requirements, resulting in large errors in the detection results, or even no detection results
At the same time, the cost of isotope determination for liquids is relatively high, and the cost of a single sample test is usually around 1,000 yuan, which greatly limits the analysis and determination of soil microbial carbon isotopes

Method used

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  • Method for determining carbon isotope content of soil microorganisms
  • Method for determining carbon isotope content of soil microorganisms
  • Method for determining carbon isotope content of soil microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for measuring soil microbial carbon isotope content, comprising the steps of:

[0034] S1: Collect 0-15cm soil samples from the field of Yangtian Village Experimental Base in Zengcheng District, Guangzhou City, Guangdong Province. After removing stones and plant roots, air-dry and pass through a 4mm sieve for later use. At the same time, fumigation and non-fumigation treatments were set, and 10 g of soil samples (by dry weight) were added to a single parallel sample. The fumigated samples were placed in a chloroform environment and cultured in a closed environment for 24 hours. Then use 50mL0.5mol / L K 2 SO 4 The solution extracts the dissolved organic carbon in the soil, and the extraction conditions are as follows: the temperature is 25° C., the rotation speed is 200 rpm, and the extraction time is 2 hours. Do not fumigate the sample directly with 50mL0.5mol / L K 2 SO 4 Solution extraction. Each treatment has 3 parallels.

[0035] S2: Take 10 mL of the f...

Embodiment 2

[0043] A method for measuring soil microbial carbon isotope content, comprising the steps of:

[0044] S1: Collect 0-15cm soil samples from the experimental base of Liuge Village, Dongli Town, Leizhou City, Zhanjiang City, Guangdong Province. After removing stones and plant roots, air-dry and pass through a 4mm sieve for later use. At the same time, fumigation and non-fumigation treatments were set, and 10 g of soil samples (by dry weight) were added to a single parallel sample. The fumigated samples were placed in a chloroform environment and cultured in a closed environment for 24 hours. Then with 50mL 0.5mol / L K 2 SO 4 The solution extracts the dissolved organic carbon in the soil, and the extraction conditions are as follows: the temperature is 25° C., the rotation speed is 200 rpm, and the extraction time is 2 hours. Do not fumigate the sample directly with 50mL 0.5mol / L K 2 SO 4 Solution extraction. Each treatment has 3 parallels.

[0045] S2: Take 10 mL of the fi...

Embodiment 3

[0069] Embodiment 3 Sensitivity detection

[0070] 1: Using commercial glucose as carbon source ( 13 The C content is 1.0977±0.0011‰), respectively set 0, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 30mg / L (in terms of carbon, the carbon content in the solution) multiple treatments, each treatment Set up 3 parallel samples. Glucose was dissolved in 50 mL of 0.5 mol / L K 2 SO 4 In the solution, simulate the extracted solution, and use mg / L (calculated as carbon) as the background value control.

[0071] 2: Put the solution obtained above into a plastic centrifuge tube, seal the mouth of the tube with a sealing strip, and pierce 3 to 4 small holes on the sealing strip of the mouth of the tube with a fine needle. Immediately use a freeze dryer for dehydration (-80°C, 72h). Immediately after the dehydration treatment, the obtained powder was placed in a sealed sample tube and stored at -20°C for future testing. During the test, take out 0.8 ~ 1.2mg of powder, add it to the tin cup for te...

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Abstract

The invention discloses a method for determining the carbon isotope content of soil microorganisms, which comprises the following steps of dividing soil samples into two groups, fumigating the first group of samples with chloroform, extracting with a potassium sulfate solution to obtain a filtrate A, and directly extracting the second group of samples without fumigation with a potassium sulfate solution to obtain a filtrate B, and measuring the total carbon content of the filtrate A and the filtrate B, respectively sealing the remaining parts, carrying out freeze drying to obtain powder A andpowder B, respectively measuring the 13C content of the powder A and the powder B, and further calculating the 13C content of the microorganisms in the soil sample. The method is simple and convenient, the carbon isotope content in a unit volume sample can be increased, a sample with low concentration can be detected, the sensitivity is high, the lowest detectable sample has the lowest concentration of 0.5 mg / L, and the cost is lower.

Description

technical field [0001] The invention belongs to the technical field of measuring soil microbial carbon, and more specifically relates to a method for measuring soil microbial carbon isotope content. Background technique [0002] The carbon cycle is at the core of global change research. In the past 200 years, due to the continuous increase of atmospheric CO2 concentration, the carbon cycle and greenhouse gas issues have been paid more and more attention. The focus of global carbon cycle research is to identify and quantify carbon sources and sinks. The soil carbon cycle is an important part of the global carbon cycle. The global average thickness of soil is only 3m, but the carbon content contained in the soil is more than twice that of the atmospheric carbon content. Therefore, changes in soil carbon content will have a significant impact on atmospheric CO 2 concentrations and the global carbon cycle. Soil microorganisms play an important role in regulating the cycle of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/24G01N27/62G01N1/34G01N1/44
CPCG01N33/24G01N27/62G01N1/34G01N1/44
Inventor 蒋新宇程炯刘平刘晓南
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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