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A method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil

A technology for phytoremediation and polluted soil, applied in the restoration of polluted soil, etc., can solve the problems of difficult competition of indigenous microorganisms, prone to functional degradation, and difficult survival of microorganisms, so as to promote the extraction of heavy metals, improve the ability of survival and colonization, and improve The effect of plant biomass

Active Publication Date: 2022-06-21
HUNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, artificially added microorganisms with repairing functions have many problems such as difficulty in survival in the environment, low repairing efficiency, difficulty in competing with indigenous microorganisms, and prone to functional degradation.

Method used

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  • A method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil
  • A method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil
  • A method of sludge biochar immobilization bacteria combined with phytoremediation of heavy metal polluted soil

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: a method for sludge biochar immobilized bacteria combined with phytoremediation of heavy metal contaminated soil, comprising the following steps:

[0030] S1. Screen out Robinia pseudoacacia with a high level of resistance to heavy metal cadmium;

[0031] S2. Screen and isolate the rhizobacteria of Robinia pseudoacacia that are resistant to various heavy metals from the restoration plants in the mining area. The rhizobacteria of Robinia pseudoacacia can be added to the rhizosphere of various plants including Robinia pseudoacacia to play a role;

[0032] S3, screening the bacteria with resistance to a variety of heavy metals obtained in step S2, and isolating single bacterial strains capable of dissolving phosphorus, fixing nitrogen, secreting plant growth hormones (IAA and ACC) and secreting siderophores;

[0033] S4, utilize potted plant test, carry out plant tie-back test to the bacteria obtained in step S3, obtain the bacteria that can promote plant bio...

Embodiment 2

[0036] Embodiment 2: a method for sludge biochar immobilized bacteria combined with phytoremediation of heavy metal contaminated soil, comprising the following steps:

[0037] S1. Screen out Robinia pseudoacacia with a high level of resistance to heavy metal cadmium;

[0038] S2. Screen and isolate the rhizobacteria of Robinia pseudoacacia that are resistant to various heavy metals from the restoration plants in the mining area. The rhizobacteria of Robinia pseudoacacia can be added to the rhizosphere of various plants including Robinia pseudoacacia to play a role;

[0039] S3, screening the bacteria with resistance to a variety of heavy metals obtained in step S2, and isolating single bacterial strains capable of dissolving phosphorus, fixing nitrogen, secreting plant growth hormones (IAA and ACC) and secreting siderophores;

[0040] S4, using the pot experiment, the bacteria obtained in the step S3 are subjected to a plant back-connection test to obtain bacteria that can pro...

Embodiment 3

[0043] Embodiment 3: a method for sludge biochar immobilized bacteria combined with phytoremediation of heavy metal contaminated soil, comprising the following steps:

[0044] S1. Screen out Robinia pseudoacacia with a high level of resistance to heavy metal cadmium;

[0045] S2. Screen and isolate the rhizobacteria of Robinia pseudoacacia that are resistant to various heavy metals from the restoration plants in the mining area. The rhizobacteria of Robinia pseudoacacia can be added to the rhizosphere of various plants including Robinia pseudoacacia to play a role;

[0046] S3, screening the bacteria with resistance to a variety of heavy metals obtained in step S2, and isolating single bacterial strains capable of dissolving phosphorus, fixing nitrogen, secreting plant growth hormones (IAA and ACC) and secreting siderophores;

[0047] S4, using the pot experiment, the bacteria obtained in the step S3 are subjected to a plant back-connection test to obtain bacteria that can pro...

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Abstract

The invention relates to a method for sludge biochar immobilization bacteria combined with plants to remediate heavy metal-contaminated soil, comprising screening plants, screening and isolating bacteria resistant to various heavy metals, and isolating the bacteria capable of dissolving phosphorus, fixing nitrogen, secreting plant growth hormone, Bacteria that secrete siderophores, promote plant biomass accumulation, root development, and growth hormone secretion, enable bacterial load fixation to sludge biochar and soil remediation steps. The invention uses sludge biochar to immobilize bacterial strains, the microorganisms are protected by biochar, and can be released slowly in the soil, effectively improving the survival and colonization ability of microorganisms in plants and rhizosphere, and greatly increasing plant biomass. Effectively reduce the heavy metal content in the soil, and at the same time promote the root extraction of repairing plants, and achieve the goal of joint repairing of heavy metal pollution in soil by plant-microbe-carbon-based materials.

Description

technical field [0001] The invention relates to the field of heavy metal soil remediation, in particular to a method for remediation of heavy metal contaminated soil by sludge biochar immobilized bacteria combined with plants. Background technique [0002] At present, there are many bioremediation technologies applied to heavy metal-contaminated soils in mining areas. Among them, the method of using plant-microbe joint remediation to achieve synergistic effect is widely recognized. However, currently artificially added microorganisms with repair functions have many problems in the environment, such as difficult survival, low repair efficiency, difficulty in competing with indigenous microorganisms, and easy functional degradation. SUMMARY OF THE INVENTION [0003] Based on the above-mentioned deficiencies of the above-mentioned prior art, it is necessary to provide a method for sludge biochar immobilization bacteria combined with phytoremediation of heavy metal contaminate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/10B09C1/105
Inventor 张轩李辉黄忠良吴子剑黄兢覃晓莉喻金兰姚雨轩谭梦娇江世林
Owner HUNAN ACAD OF FORESTRY
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