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Composition for promoting hematopoietic cell proliferation and application thereof

A technology for hematopoietic cells and compositions is applied in the field of compositions for promoting hematopoietic cell proliferation, and can solve the problems of limited sources of hematopoietic stem and progenitor cells, cell death and the like

Active Publication Date: 2021-02-23
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the source of hematopoietic stem and progenitor cells is limited, and cell death will occur in the process of culturing hematopoietic stem cells in vitro, and its in vitro expansion is still difficult to break through the big bottleneck problem

Method used

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  • Composition for promoting hematopoietic cell proliferation and application thereof
  • Composition for promoting hematopoietic cell proliferation and application thereof
  • Composition for promoting hematopoietic cell proliferation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The cells used in this experiment are TF-1 cells, human umbilical cord blood CD34+ hematopoietic stem cells, human bone marrow blood CD34+ hematopoietic stem cells, TF-1 cells are the laboratory stock, and the culture conditions are RAPI-1640 medium, 5ng / ml GM- CSF, 10% serum and 1% streptomycin / penicillin. The culture conditions of human cord blood / bone marrow blood CD34+ hematopoietic stem cells were SFEM medium, 100ng / ml SCF, 50ng / ml TPO, 50ng / ml Flt3, 50ng / ml IL-3 and 1% streptomycin / penicillin.

[0037] The proliferation of cells was observed by cck8 proliferation assay, and 1×10 cells were inoculated in 96-well plates. 5 / 100ul of TF-1 (human erythroid leukemia cells) cell suspension (100ul / well), use the small molecule compound erastin to act on TF-1 cells and fer-1 at 0, 2.5, 5, 10, 20 (μM) (0.5μmol / l) cultured TF-1 cells, after 24h, add 10ul cck8 detection solution to each well, place the culture plate in an incubator and incubate for 3h, and measure the OD va...

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Abstract

The invention provides a composition for promoting hematopoietic cell proliferation and application thereof, and relates to the technical field of hematopoietic cell proliferation. The composition forpromoting hematopoietic cell proliferation provided by the invention comprises hematopoietic cytokines, and a ferroptosis inhibitor; the hematopoietic cytokines consist of stem cell factors, Flt3 ligands, thrombopoietin, and interleukin-3; the concentration of the stem cell factors is 20-100 ng / mL; the concentration of the Flt3 ligands is 10-50 ng / mL; the concentration of the thrombopoietin is 10-50 ng / mL; the concentration of the interleukin-3 is 10-50 ng / mL; and the molar concentration of the ferroptosis inhibitor is 0.1-10 [mu] mol / L. The composition provided by the invention combines thehematopoietic cytokines and the ferroptosis inhibitor so as to promote proliferation of hematopoietic stem progenitor cells by up-regulating expression of glutathione peroxidase-4 and inhibiting generation of active oxygen.

Description

technical field [0001] The invention relates to the technical field of proliferation of hematopoietic stem and progenitor cells, in particular to a composition for promoting proliferation of hematopoietic cells and an application thereof. Background technique [0002] Hematopoietic stem and progenitor cells are adult stem cells in the blood system. They are a heterogeneous population with the ability of long-term self-renewal and the potential to differentiate into various mature blood cells. It is a type of adult stem cell with the longest and most in-depth research history, and it has important guiding significance for the study of various stem cells, including tumor stem cells. [0003] Hematopoietic stem cell transplantation has been widely used in the treatment of malignant blood diseases, non-malignant refractory blood diseases, genetic diseases and some solid tumors. However, the source of hematopoietic stem and progenitor cells is limited, and cell death will occur ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2501/125C12N2501/26C12N2501/145C12N2501/2303C12N2501/71C12N2501/999
Inventor 王立生张丹肖凤君吴祖泽聂文博王华赵丽晶
Owner ACADEMY OF MILITARY MEDICAL SCI