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Casein peptide with sleep improving effect and preparation method and application thereof

A technology of casein peptides and sleep, which is applied in the preparation method of peptides, medical preparations containing active ingredients, hydrolyzed protein ingredients, etc., and can solve problems such as complex manufacturing methods, loss of ingredients, general or ineffective effects, etc.

Inactive Publication Date: 2021-02-26
中食都庆(山东)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since drugs can damage the body, non-drug-treated health foods have become more and more choices for people with insomnia. There are many health foods on the market that are used to improve sleep, adding some expensive Chinese medicinal materials such as ginseng, which cost a lot , the effect is not fully exerted, resulting in general or ineffective effect
There are also many types of medicines that are needed, and the manufacturing methods are complicated, and many ingredients are not extracted completely, resulting in the loss of ingredients, and the sleep-promoting function of health food is invalid.

Method used

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  • Casein peptide with sleep improving effect and preparation method and application thereof
  • Casein peptide with sleep improving effect and preparation method and application thereof
  • Casein peptide with sleep improving effect and preparation method and application thereof

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preparation example Construction

[0041] The present invention provides the preparation method of the casein peptide T1 or casein peptide T2 with sleep improving effect, comprising the following steps:

[0042] 1) Homogenizing and homogenizing the casein solution to obtain a homogenate with stable properties;

[0043]2) stirring the homogenate at 48-52°C for 1-1.5 hours, adjusting the pH value to 7.0-7.4 to obtain a pretreated homogenate;

[0044] 3) enzymatically hydrolyzing the pretreated homogenate under the action of trypsin and papain in sequence, and after inactivating the enzyme, an enzymatic hydrolyzate is obtained;

[0045] 4) After removing the pigment from the enzymatic solution, pass through a 30,000Da ceramic membrane and a 1KD organic membrane in sequence, collect and dry the undercoat to obtain a casein peptide mixture;

[0046] 5) Using a Sephadex G-50 chromatographic column to roughly separate and purify the casein peptide mixture, and collect components with a peak time of 12 to 15 minutes; ...

Embodiment 1

[0074] Preparation method of novel casein peptide

[0075] Step 1. Take 500kg of casein for slurry mixing, add 5000L of deionized water to obtain a casein solution with a concentration of 10%.

[0076] Step 2. Stir the casein solution in step 1 evenly, use a colloid mill to process it for 1.2 hours, and then use a high-pressure homogenizer to process it for 2 hours under a pressure of 25 MPa to ensure that the homogenate is fine in texture and non-layered, and improve the homogeneity. The stability of the slurry.

[0077] Step 3. Feed the homogenate after high-pressure homogenization, carry out stirring treatment, and raise the temperature to 50° C., and keep it warm for 1 hour.

[0078] Step 4. Adjust the pH value of the casein solution in Step 3 to 7.2 with 0.5M NaOH solution, and keep the temperature constant.

[0079] Step 5. Add 0.6% trypsin (3.0Kg) to the pH-adjusted casein solution in step 4. After 1 hour of enzymolysis, add 0.3% papain (1.5kg) and continue the enzymo...

Embodiment 2

[0086] Crude Separation and Purification of Casein Peptides

[0087] Swelling of the gel: Weigh an appropriate amount of Sephadex G-50 gel dry powder, add excess deionized water, let it swell at room temperature for 2 days, then vacuumize to remove the air in the gel void, and fully swell for 2 hours in a boiling water bath After fully swelling, remove the small particles floating on the surface that are not easy to sink, as well as the air and bacteria inside, and wash with deionized water for 2 to 3 times for later use.

[0088] Column loading and balance: Take a clean 3×100cm chromatography column and fix it vertically on the iron stand, close the water outlet, add distilled water (about 1 / 3) into the chromatography column, pour the swollen gel while stirring Put it into the chromatography column, wait for the gel to settle completely under the action of gravity, and the column packing is completed. Note: The gel in the column cannot be stratified. If there is stratificati...

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Abstract

The invention provides a casein peptide with a sleep improving effect and a preparation method and application thereof, and belongs to the technical field of functional polypeptides. Casein is used asa raw material and prepared into a casein peptide mixture through a biological enzymolysis technology, and two bioactive peptide sequences with opium properties are obtained through multiple times ofseparation and purification. Zebra fish tests prove that the casein peptide has an ideal effect on improving sleep. Therefore, the casein peptide prepared through the method is applied to preparationof medicine for treating insomnia or preparation of health-care products or health-care food for improving sleep quality.

Description

technical field [0001] The invention belongs to the technical field of functional polypeptides, and in particular relates to a casein peptide with sleep-improving effect and its preparation method and application. Background technique [0002] Sleep is an important physiological phenomenon. Good sleep plays an important role in maintaining normal physiological activities and physical and mental health. Insomnia brings troubles to patients' physical and mental health, disturbs people's circadian rhythm, reduces their activity ability, and seriously affects patients' quality of life and quality of life. Sleep is also a metabolic process of memory cells: aging cells will be used for each arrangement of memory information, and new cells will be input for storage. These include movements, language areas, balance keys, and some past events and memories of everyday life. They are all matter, so they also exist in a physical way. [0003] If a person's long-term lack of sleep ca...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/36C07K1/34C07K1/16C12P21/06A61K38/01A61K38/17A61P25/20
CPCA61K38/00A61P25/20C07K14/4732C12P21/06
Inventor 张九勋张学军田明展张西平魏玮王慧娟王芳谷帅
Owner 中食都庆(山东)生物技术有限公司
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