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Application of artemisia dracunculus extract in stimulating broomrape seed germination

A technology of extract, Artemisia pepper, applied in the field of agriculture, achieves significant social and economic benefits, and has broad application prospects

Inactive Publication Date: 2021-03-02
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artemisia pepper has insecticidal and antibacterial effects, but its allelopathic activity has not been reported yet

Method used

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  • Application of artemisia dracunculus extract in stimulating broomrape seed germination
  • Application of artemisia dracunculus extract in stimulating broomrape seed germination
  • Application of artemisia dracunculus extract in stimulating broomrape seed germination

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A kind of application of Artemisia paprika extract in stimulating the germination of Liedang seeds, the application comprises the following steps:

[0023] (1) Extraction of Artemisia pepper extract: After Artemisia pepper was dried and shredded, it was soaked in 95% ethanol, 75% ethanol, 50% ethanol and water in turn for 48 hours, concentrated in a rotary evaporator to obtain ethanol extraction Then use n-hexane to extract the ethanol extract, concentrate it in a rotary evaporator to obtain the corresponding n-hexane extract, collect it and put it in a brown bottle for sealed storage, and put it in a refrigerator at 4°C for later use;

[0024] (2) Surface disinfection of Liedang seeds: Select an appropriate amount of Liedang seeds and put them into a 5mL centrifuge tube, add an appropriate amount of 2.1wt% sodium hypochlorite to the centrifuge tube with a pipette gun, vibrate and shake for 4 minutes, and let stand for 1 minute until precipitation ;Use a pipette to suck...

Embodiment 2

[0028] A kind of application of Artemisia paprika extract in stimulating the germination of Liedang seeds, the application comprises the following steps:

[0029] (1) Extraction of Artemisia pepper extract: After Artemisia pepper was dried and shredded, it was soaked in 95% ethanol, 75% ethanol, 50% ethanol and water in turn for 48 hours, concentrated in a rotary evaporator to obtain ethanol extraction extract, and then extract the ethanol extract with chloroform, concentrate in a rotary evaporator to obtain a chloroform extract, collect it and put it in a brown bottle for sealed storage, and place it in a refrigerator at 4°C for later use;

[0030] (2) Surface disinfection of Liedang seeds: Select an appropriate amount of Liedang seeds and put them into a 5mL centrifuge tube, add an appropriate amount of 2.1wt% sodium hypochlorite to the centrifuge tube with a pipette gun, vibrate and shake for 4 minutes, and let stand for 1 minute until precipitation ;Use a pipette to suck o...

Embodiment 3

[0034] A kind of application of Artemisia paprika extract in stimulating the germination of Liedang seeds, the application comprises the following steps:

[0035] (1) Extraction of Artemisia pepper extract: After Artemisia pepper was dried and shredded, it was soaked in 95% ethanol, 75% ethanol, 50% ethanol and water in turn for 48 hours, concentrated in a rotary evaporator to obtain ethanol extraction extract, and then extract the ethanol extract with ethyl acetate, concentrate in a rotary evaporator to obtain ethyl acetate extract, collect it and put it in a brown bottle for sealed storage, and put it in a refrigerator at 4°C for later use;

[0036] (2) Surface disinfection of Liedang seeds: Select an appropriate amount of Liedang seeds and put them into a 5mL centrifuge tube, add an appropriate amount of 2.1wt% sodium hypochlorite to the centrifuge tube with a pipette gun, vibrate and shake for 4 minutes, and let stand for 1 minute until precipitation ;Use a pipette to suck...

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Abstract

The invention discloses an application of artemisia dracunculus extract in stimulating broomrape seed germination, which comprises the following steps of: extracting an artemisia dracunculus extract,namely drying artemisia dracunculus in the sun, cutting the artemisia dracunculus into pieces, soaking, concentrating, extracting with a solvent, and concentrating to obtain artemisia dracunculus extract; disinfecting the surfaces of broomrape seeds; carrying out pre-culture, namely laying qualitative filter paper and glass fiber filter paper in a culture dish, evenly scattering the disinfected broomrape seeds on the glass fiber filter paper, carrying out sealing, and carrying out constant-temperature dark culture; carrying out a broomrape seed germination experiment, namely, adding methanol to dilute the artemisia dracunculus extract, soaking the filter paper, drying the filter paper, paving the qualitative filter paper in a culture dish, and soaking the qualitative filter paper; and putting the dried filter paper on the qualitative filter paper, selecting pre-cultured broomrape seeds, sealing, and carrying out constant-temperature dark culture. The artemisia dracunculus extract can efficiently stimulate broomrape seeds to germinate in advance, broomrape suicide germination is caused due to the fact that hosts cannot be found in time for adsorption life of the seeds, and the artemisia dracunculus extract can be effectively used for biological control work of broomrape parasitic weeds.

Description

technical field [0001] The invention relates to a method for promoting seed germination, in particular to the application of an Artemisia pepper extract in stimulating the germination of Liedang seeds, which belongs to the field of agricultural technology. Background technique [0002] Liedang is a kind of parasitic plant belonging to Liedangaceae, which often parasitizes the roots of plants. Liedang roots form haustoria and invade the host root, which will absorb nutrients and water in the host plant, hinder the growth of the plant, and make the host plant The yield decline, especially to the Cucurbitaceae, Solanaceae, Compositae, Umbelliferae, Brassicaceae growth has a greater impact. The seeds of Liedang are thin and small, and the amount of fruit is amazing. It can survive in the soil for up to ten years. Widely distributed in western Asia, the Mediterranean region and eastern Africa. There are about 23 species of plants in the genus Liedang in my country, mainly distr...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P21/00A01C1/00A01C1/08A01C1/04
CPCA01C1/00A01C1/044A01C1/046A01C1/08A01N65/12
Inventor 赵娜娜
Owner XINJIANG AGRI UNIV
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