Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting genes related to peripheral T cell lymphoma inside and outside node and library building method

A technology for detection kits and lymphoma, applied in biochemical equipment and methods, microbe measurement/testing, chemical libraries, etc., can solve the problems of high cost and limited research

Pending Publication Date: 2021-03-02
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic PTCLs genomics research is limited, and there are certain differences in the results of genetic testing in small samples and different populations; genetic testing itself also has some limitations, and its cost is relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting genes related to peripheral T cell lymphoma inside and outside node and library building method
  • Kit for detecting genes related to peripheral T cell lymphoma inside and outside node and library building method
  • Kit for detecting genes related to peripheral T cell lymphoma inside and outside node and library building method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] The composition of the kit in the embodiment of the present invention is shown in Table 3, using Diying biological products (article numbers L1001A, LP2004A, C1001A, CP3004A, Y1016A).

[0088] Table 4

[0089]

[0090] The design method of the capture probe is:

[0091] S1. Extract the exon coordinates of the target gene according to the database, and the coordinates of the exon region are shown in Figure 1;

[0092] S2. Intercept 1-120 bp of an exon region as the first probe unit, intercept 60-180 bp of the exon region as the second probe unit, and intercept 120-240 bp of the exon region as the third probe unit For the probe unit, 180-300 bp of the exon region is intercepted as the fourth probe unit, which is successively intercepted to obtain the probe of the exon region;

[0093] S3. Evaluate the nucleotide sequence of the probe, and the evaluated parameters include: Tm value, GC content, Hairpin free energy, sequence complexity and homology within the genome; ...

Embodiment 2

[0191] 1. Using the kit and method of Example 1 of the present invention to detect 4 samples, the experimental results and data obtained are as follows. The sample information is shown in Table 16:

[0192] Table 16

[0193] Sample No. Concentration (ng / μL) sample type Sample description 1 19.3 dna Clinical diagnosis of PTCL 2 12.8 dna Clinical diagnosis of PTCL 3 68.2 dna Clinical diagnosis of PTCL 4 5.42 dna Clinical diagnosis of PTCL

[0194] 2. The experimental results after fragmentation are shown in Table 17:

[0195] Table 17

[0196]

[0197]

[0198] Remarks: 1) The interruption time is 205s; 2) The target fragmentation length is 150bp.

[0199] The quality inspection results after fragmentation are as follows: Figure 3-5 shown. in, image 3 is the fragmented quality inspection result of sample number 1, Figure 4 is the fragmented quality inspection result of sample number 2, Figure 5 It is the f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of gene mutation detection, in particular to a kit for detecting genes related to T cell lymphoma inside and outside a node and a library building method. The kit for detecting genes related to T cell lymphoma inside and outside a node comprises a capture probe for 76 target genes. The kit provided by the invention adopts liquid phase capture for library construction, and has the advantages of library construction, high coverage rate, high comparison rate, good uniformity, simple operation and the like.

Description

technical field [0001] The invention relates to the field of gene mutation detection, in particular to a detection kit and a method for building a library of genes related to peripheral T-cell lymphoma in the nodal area. Background technique [0002] Lymphoma is a hematological malignancy originating from lymph nodes and lymphoid tissues, with complex pathological types and high incidence. Traditional lymphoma is mainly divided into two categories: Hodgkin's lymphoma (Hodgkin's lymphoma) and non-Hodgkin's lymphoma (NHL), and the latter can be divided into B-cell NHL and T-cell NHL. category. Peripheral T-cell lymphomas (PTCLs) originate from postthymic mature T cells or NK / T cells and are highly heterogeneous and aggressive. The most common primary nodal T-cell lymphomas include peripheral T-cell tumors not specified (PTCL-NOS), angioimmunoblastic lymphoma (AITL) and anaplastic large cell tumor (ALCL), accounting for about 75% of all PTCLs cases. The incidence of PTCLs in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6874C40B50/06
CPCC12Q1/6886C12Q1/6874C40B50/06C12Q2600/156C12Q2525/191C12Q2531/113C12Q2535/122C12Q2521/501
Inventor 赵维莅纪濛濛黄耀慧付迪熊慧刘以哲俞浩崔帆
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com