A kind of electrochemiluminescence sensor for detecting oligonucleotide and preparation method thereof

An oligonucleotide and electrochemical technology, which is applied in the field of DNA nanometers, can solve the problems of low light efficiency of electrochemiluminescent beacon materials and the difficulty of ultra-trace sample detection, so as to achieve the goal of being less susceptible to external environmental interference and good biophase capacitive and stability effects

Active Publication Date: 2022-07-01
HUAZHONG AGRI UNIV
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Problems solved by technology

[0004] Although electrochemiluminescence sensors have many advantages, the following problems still exist in the detection of complex samples such as biological tissues, saliva, and body fluids: the light efficiency of electrochemiluminescent beacon substances is low, and they are easily affected by the environment (pH, temperature, etc.), Difficult to achieve detection of ultra-trace samples

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  • A kind of electrochemiluminescence sensor for detecting oligonucleotide and preparation method thereof
  • A kind of electrochemiluminescence sensor for detecting oligonucleotide and preparation method thereof
  • A kind of electrochemiluminescence sensor for detecting oligonucleotide and preparation method thereof

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preparation example Construction

[0031] see figure 1 As shown, the embodiment of the present invention provides a preparation method of an electrochemiluminescence sensor for detecting oligonucleotides, including:

[0032] S1. Prepare mesoporous silica, load ruthenium bipyridine in the pores of the mesoporous silica, and use ssDNA to seal the pores of the mesoporous silica to obtain ssDNA-Ru-MSNs, where the diameter of the mesoporous silica is 93-104nm, and the diameter of the surface pores is 3.5-4nm.

[0033] S2. Prepare Product DNA, add template DNA and oligonucleotide to be detected into isothermal amplification buffer, and then add cleavage endonuclease, polymerase and deoxyribonucleoside triphosphate to react to obtain Product DNA.

[0034] S3. Mix ssDNA-Ru-MSNs and Product DNA to prepare a specific conjugate, and modify the specific conjugate on a glassy carbon electrode to obtain an oligonucleotide electrochemiluminescence sensor. The ssDNA and Product DNA are complementary chains to each other .

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Abstract

The invention discloses an electrochemiluminescence sensor for detecting oligonucleotides and a preparation method thereof, and relates to the field of DNA nanotechnology. Ruthenium pyridine, using ssDNA to seal the pores of mesoporous silica to obtain ssDNA-Ru-MSNs; mixing ssDNA-Ru-MSNs with Product DNA and then modifying it on the electrode to obtain an oligonucleotide electrochemiluminescence sensor, ssDNA-Ru-MSNs and Product DNA DNA is complementary strands to each other. The electrochemiluminescence sensor includes an electrode, the electrode is modified with a specific binding substance of ssDNA-Ru-MSNs and Product DNA, and ssDNA and Product DNA are complementary chains to each other. The present invention can perform ultra-trace detection on complex samples, and is not easily disturbed by the external environment.

Description

technical field [0001] The invention relates to the field of DNA nanotechnology, in particular to an electrochemiluminescence sensor for detecting oligonucleotides and a preparation method thereof. Background technique [0002] Electrochemiluminescence (ECL) is a process of triggering chemiluminescence through electrochemical methods. ECL combines the advantages of electrochemistry and chemiluminescence, and has high stability, high sensitivity, controllable experimental system, wide detection range, and short detection time. Features. The principle of ECL is to apply a voltage to the reactant and perform electrochemical regeneration on the electrode. The generated regenerator itself or reacts with the substrate in the system to form an excited state substance, and then energy relaxation occurs, and the excited state substance releases a photon Reaching the ground state, the optical radiation generated from the excited state to the ground state can be detected by a detector...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/416G01N21/76
CPCG01N27/3276G01N27/4163G01N21/76
Inventor 韩鹤友丁凡王文静李允
Owner HUAZHONG AGRI UNIV
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