Method for promoting chlorella pyrenoidosa growth by using Volatile fatty acids

A technology of Chlorella pyrenoidosa and volatile fatty acids, which is applied in the field of realizing short-term biomass growth of microalgae, can solve problems such as ecological environment hazards and low energy conversion rate, achieve high conversion rate, wide sources, and relieve energy shortage. effect of the problem

Active Publication Date: 2021-03-09
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the excessive VFAs accumulated in the fermentation waste liquid are not used rationally, not only will the energy production process be inhibited and the energy conversion rate will be low, but also these volatile acids with a pungent smell are potentially harmful to the ecological environment.

Method used

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  • Method for promoting chlorella pyrenoidosa growth by using Volatile fatty acids
  • Method for promoting chlorella pyrenoidosa growth by using Volatile fatty acids

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Experimental program
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Effect test

Embodiment 1

[0025] (1) Chlorella pyrenoidosa was cultured with BG-11 medium as the basal medium (see Table 1 for its formula), and its optical density was measured every day to observe the growth status of the microalgae and draw a growth curve.

[0026] (2) After the microalgae grow to the logarithmic growth phase, centrifuge the algae solution at 4000 r / min for 10 min, discard the supernatant, and resuspend the algae cells in BG-11 medium as the seed solution.

[0027] (3) To induce the rapid growth of algal cells, a 4 g / L mixed VFAs solution was prepared, in which the concentration ratio of acetic acid, propionic acid and butyric acid was 6: 3: 1. Take the mixed VFAs solution and add it to the seed solution prepared in step (2). The inoculated seed solution has an optical density value of 0.2 at 680 nm, and culture it at 25°C in the dark for 5 days. After being inoculated, Chlorella pyrenoidosa entered the logarithmic growth phase directly, and at 0.84 d -1 The specific growth rate ac...

Embodiment 2

[0029] (1) Chlorella pyrenoidosa was cultured with BG-11 medium as the basal medium (see Table 1 for its formula), and its optical density was measured every day to observe the growth status of the microalgae and draw a growth curve.

[0030] (2) After the microalgae grow to the logarithmic growth phase, centrifuge the algae solution at 4000 r / min for 10 min, discard the supernatant, and resuspend the algae cells in BG-11 medium as the seed solution.

[0031] (3) To induce the rapid growth of algal cells, a mixed VFAs solution of 8 g / L was prepared, in which the concentration ratio of acetic acid, propionic acid and butyric acid was 6: 1: 3. Take the mixed VFAs solution and add it to the seed solution prepared in step (2). The inoculated seed solution has an optical density value of 0.2 at 680 nm, and culture it at 25°C in the dark for 5 days. After being inoculated, Chlorella pyrenoidosa enters the logarithmic growth phase directly, and at 0.80 d -1 The specific growth rate ...

Embodiment 3

[0033] (1) Chlorella pyrenoidosa was cultured with BG-11 medium as the basal medium (see Table 1 for its formula), and its optical density was measured every day to observe the growth status of the microalgae and draw a growth curve.

[0034] (2) Microalgae grow to the logarithmic growth phase, centrifuge the algae solution at 4000 r / min for 10 min, discard the supernatant, and resuspend the algae cells in BG-11 medium as the seed solution.

[0035](3) To induce the rapid growth of algal cells, a mixed VFAs solution of 8 g / L was prepared, in which the concentration ratio of acetic acid, propionic acid and butyric acid was 4: 5: 1. Take the mixed VFAs solution and add it to the seed solution prepared in step (2). The inoculated seed solution has an optical density value of 0.2 at 680 nm, and culture it at 25°C in the dark for 5 days. After being inoculated, Chlorella pyrenoidosa entered the logarithmic growth phase directly, and at 0.91 d -1 The specific growth rate achieved t...

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Abstract

The invention belongs to the technical field of microalgae biology, and particularly relates to a method for promoting chlorella pyrenoidosa growth by using Volatile fatty acids. According to the present invention, a BG-11 culture medium is used as the base culture medium of chlorella pyrenoidosa, the algae liquid is subjected to centrifugal concentration when the microalgae grows to the logarithmic growth phase, 4-10g/L mixed Volatile Fatty Acids (VFAs) comprising acetic acid, propionic acid and butyric acid are added, the optical density value of the inoculated seed liquid at 680nm is 0.2-1.0, and the obtained material is placed at a temperature of 24-26 DEG C and is cultured for 2-5 days under a dark condition; and with the method, the growth period of heterotrophic chlorella pyrenoidosa can be significantly shortened, the microalgae can obtain the highest biomass concentration within two days, the rapid accumulation of biomass can be achieved, the conversion efficiency can be improved, the high culture cost problem caused by the too long culture period in the actual production can be reduced, and the new idea is provided for the large-scale production of oil production from microalgae.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for promoting the growth of Chlorella pyrenoidosa by using volatile fatty acids. A method for heterotrophic culture of mixed volatile fatty acids with external carbon source to achieve short-term biomass growth of microalgae. Background technique [0002] In today's society, with the rapid development of the global economy, the demand for fuel has increased dramatically. The release of a large amount of carbon dioxide during the combustion of traditional fossil fuels causes the greenhouse effect, making global warming, ecological environment pollution, and human health increasingly prominent. Seeking an economical and environmentally friendly renewable energy to replace fossil fuels is the best way to solve this problem. best means. As a clean renewable energy source, biodiesel has great potential for development. However, biodiesel in China faces th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 宋明明苏琨洋刘娜李雪于泽
Owner QILU UNIV OF TECH
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