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Microfluidic perfusion culture device and perfusion method thereof

A technology of perfusion culture and microfluidics, which is applied in the field of biomedical detection, can solve the problem of fewer samples, and achieve the effects of reducing waste, reducing cost and saving space

Active Publication Date: 2021-03-12
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Traditional microorganism / cell culture methods cannot meet the needs of space life science research, and microfluidic chips are characterized by miniaturization, less sample consumption, and high throughput, controllable microvalve micropump fluid control and flexible The unique microchannel design is conducive to the construction of a variety of space biological effect models, which can meet the various needs of manned space missions

Method used

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  • Microfluidic perfusion culture device and perfusion method thereof
  • Microfluidic perfusion culture device and perfusion method thereof
  • Microfluidic perfusion culture device and perfusion method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Such as figure 1 As shown, the present invention provides a microfluidic perfusion culture device, including a fluid control unit, a culture chamber unit 1, and a base liquid circuit unit 2. The fluid control unit connects the culture medium to the base liquid circuit unit through a pump body, and the base The liquid circuit unit controls the selection of the liquid circuit through the on-off of the solenoid valve 21, so that the culture medium flows into the culture chamber 11, and the circulation perfusion culture of the culture chamber is realized.

[0043] The fluid control unit includes a pump body connected to the pump channel 22 of the base liquid circuit unit, a liquid inlet pool connected to the liquid inlet channel 23 of the base liquid circuit unit, and a waste liquid bag connected to the liquid outflow channel 24 of the base liquid circuit unit. Wherein, the pump body can be a syringe pump, a peristaltic pump or a pressure pump.

[0044] A culture chamber i...

Embodiment 2

[0051] The invention provides a perfusion method for a microfluidic perfusion culture device, comprising the following steps:

[0052] a. Sterilize the culture chamber; sterilization methods include but are not limited to the following methods: alcohol, ultraviolet light or gas sterilization.

[0053] b. Place the target culture microorganism or cell freeze-dried powder in the culture chamber in advance, seal the contact surfaces of the culture chamber unit and the base liquid circuit unit; align the upper cover plate with the culture chamber layer and install the filter device in sequence 1. Gasket, which is fixed on the chip substrate by screws. Microbial powder or cell freeze-dried powder is placed in the culture chamber in advance. The gasket is used to seal the channel and the contact surface of the upper and lower layers to prevent leakage.

[0054] c. Connect the fluid control unit to the pipeline of the solenoid valve, set the opening and closing procedures and cycle t...

Embodiment 3

[0059] Such as Figure 2-4 As shown, the present invention provides another multi-control microfluidic perfusion culture device, including a fluid control unit, a culture chamber unit 1, and a base fluid circuit unit 2. The fluid control unit connects the culture medium to the base fluid channel through a pump body unit, the base liquid circuit unit controls the medium inflow and outflow of the culture chamber unit, the base liquid circuit unit is provided with four solenoid valves 21, and the solenoid valve A is connected with the solenoid valve B between the four solenoid valves, and the solenoid valve B is connected with the solenoid valve B. The solenoid valves C and D are connected, and the selection of the liquid path is controlled by the on-off of the four solenoid valves, so as to realize the circulation perfusion culture of the culture chamber.

[0060] The culture chamber unit is provided with a culture chamber 11, the side of the culture chamber is provided with a l...

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Abstract

The invention provides a microfluidic perfusion culture device, and the device comprises a fluid control unit, a culture chamber unit and a substrate liquid path unit; the fluid control unit connectsa culture medium into the substrate liquid path unit through a pump body, and the substrate liquid path unit controls liquid path selection through on-off of an electromagnetic valve, so the culture medium flows into a culture chamber; and circulating perfusion culture of the culture chamber is realized. According to the system, waste of manpower and resources can be reduced, shunting control overliquid, long-time high-flux perfusion culture of microorganisms or cells and real-time observation and detection are automatically achieved, culture and detection of the microorganisms or the cells are easy to operate, space is saved, and the system can be used in the ground or aerospace microgravity environment.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and in particular relates to a microfluidic perfusion culture device integrating a syringe pump and a solenoid valve and a perfusion method thereof. Background technique [0002] Traditional microbial or cell culture methods are mostly adherent and two-dimensional growth, mainly in vitro proliferation of cells in devices such as petri dishes / flasks. Although the traditional culture method has many advantages, its operation process is cumbersome, time-consuming and costly. Petri dishes are too large for cells and microorganisms, and it is difficult to quantitatively control related parameters. Under objective circumstances, they cannot accurately reflect the real growth conditions, thereby reducing the reliability of cell and microorganism research. [0003] Microfluidic chip technology integrates the sample preparation, enrichment, reaction, separation, and detection units in the process of b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/36C12M3/00
CPCC12M23/16C12M41/48C12M41/00C12M29/10C12M35/04
Inventor 吕雪飞王文甲徐建栋邓玉林任培
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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