Special culture medium for subculture of artemisia selengensis adventitious buds and preparation method thereof
A subculture and culture medium technology, applied in the field of agricultural biology, can solve problems such as complicated operation procedures, high production costs of tissue culture seedlings, and influence on the enthusiasm of tissue culture seedlings, and achieve the goal of simplifying operation procedures, reducing sterilization facilities and energy The effect of investing and saving the sterilization link
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[0035] The present invention also provides a preparation method of a special culture medium for the subculture of Artemisia quinoa adventitious buds, comprising the following steps:
[0036] (1) Bitter gourd is processed to obtain bitter gourd juice;
[0037] (2) adding 6-BA and NAA to the basal medium to obtain a basal medium with a growth regulation function;
[0038] (3) After mixing bitter gourd juice, basal medium with growth regulating function, sucrose and agar, constant volume, heating until the agar melts, to obtain a special medium for subculture of Artemisia quinoa adventitious buds.
[0039] In the present invention, the preparation method of the balsam pear juice is as follows: wash the balsam pear with tap water, remove seeds, chop, put into a pot with water and cook until the balsam pear is overripe, then filter with gauze to obtain the balsam pear juice.
[0040] In the present invention, the pot for boiling bitter gourd is preferably a refined steel pot.
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experiment example 1
[0049] Experimental example 1 optimizes the amount of basal medium MS in the special medium for the subculture of Artemisia chinensis adventitious buds
[0050]Using single factor test method. According to the proportion of MS medium added to the basic medium in the preparation of 1 liter of basic medium, four treatments of 1 / 5MS, 1 / 4MS, 1 / 3MS, and 1 / 2MS were respectively set, and MS medium was used as the control. Use purified water to successively set the medium for the subculture of Artemisia quinoa adventitious buds as follows: (1) 1 / 5MS medium, 6-BA 0.02mg / L, NAA 0.02mg / L, sucrose 25g / L, agar 6.5g / L 1 / 5MS Adventitious Bud Subculture Medium of Artemisia quinoa composed of pH 5.8; (2) 1 / 4MS medium, 6-BA 0.02mg / L, NAA 0.02mg / L, sucrose 25g / L, agar 6.5g / L 1 / 3MS Adventitious bud subculture medium of Artemisia quinoa composed of pH 5.8; (3) 1 / 3MS medium, 6-BA 0.02mg / L, NAA 0.02mg / L, sucrose 25g / L, agar 6.5g / L 1 / 3MS adventitious bud subculture medium of Artemisia quinoa with p...
experiment example 2
[0054] Experimental example 2 optimizes the amount of balsam pear used in the basal medium in the special medium for subculture of Artemisia quinoa adventitious buds
[0055] According to the addition amount of balsam pear, five treatments of 100g / L, 150g / L, 200g / L, 250g / L, and 300g / L were set, and no balsam pear was used as a control, cultured with MS Artemisia quinoa adventitious bud subculture medium, and pure Water preparation medium. When preparing the medium, add 6-BA 0.02mg / L, NAA 0.02mg / L, sucrose 25g / L, and agar 6.5g / L in sequence according to different bitter melon additions to prepare: (1) Bitter melon content 100g / L (2) Artemisia quinoa adventitious bud subculture medium with 150g / L balsam pear content; (3) Artemisia quinoa adventitious bud subculture medium with 200g / L content; (4) Bitter melon content 250g / L (5) the adventitious bud subculture medium of Artemisia quinoa content 300g / L of bitter melon; (6) control CK1 is non-sterilized, non-bitter melon adventiti...
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