Application of eurycomanone in preparation of medicine for preventing and treating acute kidney injury

A technology for acute kidney injury and ketone, which is applied in the field of preparation of drugs for the prevention and treatment of acute kidney injury with ketone, and can solve problems such as unreported

Active Publication Date: 2021-03-16
XIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field of acute kidney injury treatment, ther...

Method used

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  • Application of eurycomanone in preparation of medicine for preventing and treating acute kidney injury
  • Application of eurycomanone in preparation of medicine for preventing and treating acute kidney injury
  • Application of eurycomanone in preparation of medicine for preventing and treating acute kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Preparation of Tongkat Ali Extract.

[0027] 1. Implementation plan

[0028] Pulverize Tongkat Ali, extract with aqueous solution or alcoholic solution one or more times, filter while hot, combine extracts, concentrate to obtain Tongkat Ali extract. Wherein, the amount of aqueous solution, or low-concentration ethanol aqueous solution, or low-concentration methanol aqueous solution is 5-25 times the weight of Tongkat Ali; the extraction time is 1h-3h. The obtained extract has a concentration of 330mg / ml.

Embodiment 2

[0029] Example 2: Preparation and identification of ketones.

[0030] 1. Implementation plan

[0031] The diterpenoid components in the Tongkat Ali extract include one or more of pyranol-2-O-β-D-glucopyranoside, pyrylone, and pyryl alcohol, wherein pyryl The content of alcohol-2-O-β-D-glucopyranoside is 0.1% to 5%, the content of pyroxylenone is 0.1% to 5%, and the content of pyranyl alcohol is 0.1% to 5%. In order to clarify the pharmacological activity of ketone, we isolated ketone, chemical formula C 20 h 24 o 9 , molecular weight 408.403, CAS accession number 84633-29-4.

[0032] The specific preparation method is as follows: according to the previously obtained tongkat ali extract, ethanol precipitation with 75-97% content, repeated silica gel column chromatography, and recrystallization to obtain the ketone monomer, the purity of which is over 95% through HPLC analysis.

[0033] 2. Implementation results

[0034] Through nuclear magnetic resonance spectroscopy (Nucle...

Embodiment 3

[0035] Example 3: Tongkat Ali extract and ketanthamone relieve IRI-induced AKI.

[0036] 1. Implementation plan

[0037] Male C57BL / 6 mice at the age of 8-10 weeks were randomly divided into control group, ischemia-reperfusion injury (Ischemia-reperfusion injury, IRI) treatment group, eutonerone (EN) + IRI treatment group (EN treatment group) , Tongkat Ali (EL) extract+IRI treatment group (EL treatment group), 5 rats in each group. The mice in each group were treated as follows:

[0038] Control group: room temperature, 3% pentobarbital sodium anesthetized the mice with 1ml / kg body weight, the mice were simply laparotomed at the midline of the abdomen to find the renal pedicle, but the renal pedicle was not clamped, and then sutured layer by layer.

[0039] IRI treatment group: the anesthesia and surgical approach were the same as above. The bilateral renal pedicles of the mice were clamped for 30 minutes with a micro arterial clip, and the body temperature was maintained at...

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Abstract

The invention discloses application of eurycomanone in preparation of a medicine for preventing and treating acute kidney injury, and belongs to the field of biomedicines. According to the invention,animal in-vivo research finds that eurycoma longifolia extract and the eurycomanone can effectively relieve acute renal function decline and renal tissue injury of mice due to ischemia-reperfusion injury. The eurycomanone is a main active ingredient in eurycoma longifolia. In-vivo and in-vitro studies find that the eurycomanone protects the mitochondrial function by activating an AMPK/PGC-1[alpha]signal path, so that renal inflammatory response mediated by proximal renal tubular epithelial cell injury is relieved. Therefore, the eurycoma longifolia aqueous extract and the eurycomanone have anobvious kidney protection effect, do not have lethal toxic and side effects, and can be used for preparing acute kidney injury resisting medicines.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of Eurycomanone (Eurycomanone, EN) in the preparation of medicines for preventing and treating acute kidney injury. Background technique [0002] Acute kidney injury (AKI) refers to a group of clinical syndromes caused by short-term renal function damage caused by various reasons. With the aging of the population, the increase of invasive examinations, and the widespread use of contrast agents and chemotherapy drugs, the morbidity and mortality of AKI are increasing year by year, and the ICU mortality rate is as high as 50%. In recent years, a large number of epidemiological studies have shown that even if the damage is extremely mild, AKI patients may still have abnormal kidney structure and develop chronic kidney disease (CKD) or even end-stage renal disease (ESRD). Once AKI occurs, there is almost no intervention that can prevent the development of kidney damage...

Claims

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Application Information

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IPC IPC(8): A61K31/366A61P13/12C07D493/10
CPCA61K31/366A61P13/12C07D493/10
Inventor 刘泽刘芳张远芳李勇
Owner XIANGNAN UNIV
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