Use of neutrophil gelatinase-associated lipocalin to promote crossing of therapeutic antibodies across blood-brain barrier
A technology of lipocalin and neutrophils, which is applied in the field of tumor treatment, can solve the problems such as the difficulty of large-scale complete resection of surgery, and achieve the effects of strong clinical transformation significance, improved effect, and good therapeutic effect
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[0017] The present invention will be further technically described below with reference to the accompanying drawings and examples.
[0018] 1. Synthesis of Monoclonal Antibody (mAb) Nanocapsules
[0019] Cetuximab solution (ERBITUX, Merck, Darmstadt, Germany) (5 mg / mL) was diluted to 1 mg / mL with phosphate buffer (20 mM, pH=7.4) in an ice bath. N-(3-aminopropyl)methacrylamide (APM) was added to the antibody solution and mixed at 4°C for 10 minutes. 2-Methacryloyloxyethylphosphorylcholine (MPC) monomer and diallyl disulfide (DADS) were then added to the solution. The molar ratio of MPC:APM:crosslinker was adjusted to 50:5:1. Ammonium persulfate (APS) and N,N,N,N'-tetramethyldiamine (TEMED) were then added to the reaction solution to initiate radical polymerization. Polymerization was performed under nitrogen at 4°C for 2 hours to form nanocapsules. The synthesized nanocapsules were purified with a hydrophobic interaction column (Phenyl-Sepharose 4BCL) and dialyzed against P...
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